Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)
To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains the n...
| Autores principales: | , , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
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International Center for Tropical Agriculture
1995
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| Acceso en línea: | https://hdl.handle.net/10568/55850 |
| _version_ | 1855539959313727488 |
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| author | Sarria, Rodrigo A. Balcázar, N Destéfano Beltrán, L.J.C. Roca, W. |
| author_browse | Balcázar, N Destéfano Beltrán, L.J.C. Roca, W. Sarria, Rodrigo A. |
| author_facet | Sarria, Rodrigo A. Balcázar, N Destéfano Beltrán, L.J.C. Roca, W. |
| author_sort | Sarria, Rodrigo A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains the npt II, gus, and bar genes. Inoculations were carried out on cotyledonary leaves obtained from somatic embryos of cassava cultivar M Peru 1983. Following co-cultivation, somatic embryos were induced in a selection medium, supplemented with 16-32 mg/l of Basta. Several Basta-resistant plantlets were obtained. Positive lines were screened, using the polymerase chain reaction (PCR), together with specific primers for the gus and products analyzed on Southern blots under highly stringent conditions. Only lines showing the presence of both genes were transplanted to pots. Genomic Southern analysis of these plants are new under way |
| format | Conference Paper |
| id | CGSpace55850 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace558502024-01-09T09:49:37Z Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) Sarria, Rodrigo A. Balcázar, N Destéfano Beltrán, L.J.C. Roca, W. manihot esculenta plant breeding genetic transformation agrobacterium tumefaciens embryonic development gene expression fitomejoramiento transformación genética desarrollo embrionario expresión génica To help solve breeding problems in cassava that have long eluded conventional strategies, a routine transformation protocol is esential. The agropine-mannopine nondisarmed strain of Agrobacterium tumefaciens (CIAT 1182) was selected and used with the binary vector pGV1040. This vector contains the npt II, gus, and bar genes. Inoculations were carried out on cotyledonary leaves obtained from somatic embryos of cassava cultivar M Peru 1983. Following co-cultivation, somatic embryos were induced in a selection medium, supplemented with 16-32 mg/l of Basta. Several Basta-resistant plantlets were obtained. Positive lines were screened, using the polymerase chain reaction (PCR), together with specific primers for the gus and products analyzed on Southern blots under highly stringent conditions. Only lines showing the presence of both genes were transplanted to pots. Genomic Southern analysis of these plants are new under way 1995 2015-01-28T14:21:53Z 2015-01-28T14:21:53Z Conference Paper https://hdl.handle.net/10568/55850 en Open Access International Center for Tropical Agriculture Sarria, Rodrigo A.; Balcázar, N.; Destéfano Beltrán, L.J.C.; Roca, William M. 1995. Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz). In: International Scientific Meeting (2, 1984, Bogor, Indonesia). The Cassava Biotechnology Network: Proceedings. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. v. 1, p. 241-244. (Working document no. 150) |
| spellingShingle | manihot esculenta plant breeding genetic transformation agrobacterium tumefaciens embryonic development gene expression fitomejoramiento transformación genética desarrollo embrionario expresión génica Sarria, Rodrigo A. Balcázar, N Destéfano Beltrán, L.J.C. Roca, W. Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title | Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title_full | Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title_fullStr | Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title_full_unstemmed | Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title_short | Progress in Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) |
| title_sort | progress in agrobacterium mediated transformation of cassava manihot esculenta crantz |
| topic | manihot esculenta plant breeding genetic transformation agrobacterium tumefaciens embryonic development gene expression fitomejoramiento transformación genética desarrollo embrionario expresión génica |
| url | https://hdl.handle.net/10568/55850 |
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