Selecting heterozygous parents and single-dose markers for genetic mapping in cassava
Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Seven...
| Main Authors: | , , , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Center for Tropical Agriculture
1995
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/55846 |
| _version_ | 1855525597128687616 |
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| author | Gómez, R. Angel, F. Bonierbale, Merideth W. Rodríguez, F. Tohme, Joseph M. Roca, W. |
| author_browse | Angel, F. Bonierbale, Merideth W. Gómez, R. Roca, W. Rodríguez, F. Tohme, Joseph M. |
| author_facet | Gómez, R. Angel, F. Bonierbale, Merideth W. Rodríguez, F. Tohme, Joseph M. Roca, W. |
| author_sort | Gómez, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Seventy primers were evaluated in the largest progeny, and 120 markers were generated; of these, 93 were present in only one parent and 27 were present in both. Polymorphic bands were scored for segregation in the progeny as present or absent. Of the 93 polymorphic markers detected in the largest progeny, 67 segregated 1:1 and 9 showed ratios different to 1:1, indicating that they could be double- or triple-dose with chromatid segregation; 17 showed segregation distortions |
| format | Conference Paper |
| id | CGSpace55846 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace558462024-01-09T09:48:37Z Selecting heterozygous parents and single-dose markers for genetic mapping in cassava Gómez, R. Angel, F. Bonierbale, Merideth W. Rodríguez, F. Tohme, Joseph M. Roca, W. manihot esculenta genetic markers segregation genetic maps marcadores genéticos segregación mapas genéticos Random amplified polymorphic DNA (RAPD) analysis was performed on three F1 populations obtained from intraspecific controlled crosses of cassava. Segregation of the RAPD fragments was analyzed to determine if these can serve as genetic markers in cassava for subsequent use in linkage analysis. Seventy primers were evaluated in the largest progeny, and 120 markers were generated; of these, 93 were present in only one parent and 27 were present in both. Polymorphic bands were scored for segregation in the progeny as present or absent. Of the 93 polymorphic markers detected in the largest progeny, 67 segregated 1:1 and 9 showed ratios different to 1:1, indicating that they could be double- or triple-dose with chromatid segregation; 17 showed segregation distortions 1995 2015-01-28T14:21:53Z 2015-01-28T14:21:53Z Conference Paper https://hdl.handle.net/10568/55846 en Open Access International Center for Tropical Agriculture Gómez, Rocio; Angel, Fernando; Bonierbale, Merideth W.; Rodríguez, Fernando; Tohme M., Joseph; Roca, William M. 1995. Selecting heterozygous parents and single-dose markers for genetic mapping in cassava. In: International Scientific Meeting (2, 1984, Bogor, Indonesia). The Cassava Biotechnology Network: Proceedings. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. v. 1, p. 113-124. (Working document no. 150) |
| spellingShingle | manihot esculenta genetic markers segregation genetic maps marcadores genéticos segregación mapas genéticos Gómez, R. Angel, F. Bonierbale, Merideth W. Rodríguez, F. Tohme, Joseph M. Roca, W. Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title | Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title_full | Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title_fullStr | Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title_full_unstemmed | Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title_short | Selecting heterozygous parents and single-dose markers for genetic mapping in cassava |
| title_sort | selecting heterozygous parents and single dose markers for genetic mapping in cassava |
| topic | manihot esculenta genetic markers segregation genetic maps marcadores genéticos segregación mapas genéticos |
| url | https://hdl.handle.net/10568/55846 |
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