Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos
Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen...
| Main Authors: | , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Center for Tropical Agriculture
1993
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/55689 |
| _version_ | 1855537830458032128 |
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| author | Cataño C, ML Mornan, K Plazas, J Roca, W. |
| author_browse | Cataño C, ML Mornan, K Plazas, J Roca, W. |
| author_facet | Cataño C, ML Mornan, K Plazas, J Roca, W. |
| author_sort | Cataño C, ML |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be presented. |
| format | Conference Paper |
| id | CGSpace55689 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace556892024-01-09T09:49:27Z Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos Cataño C, ML Mornan, K Plazas, J Roca, W. manihot esculenta anther culture embryo culture methods culture media cultivo de anteras cultivo de embriones métodos medio de cultivo Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be presented. 1993 2015-01-28T14:21:41Z 2015-01-28T14:21:41Z Conference Paper https://hdl.handle.net/10568/55689 en Open Access International Center for Tropical Agriculture Cataño C., Martha Lucía; Mornan, K.; Plazas, J.; Roca, William M. 1993. Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos. In: Roca, William M.; Thro, Ann Marie (eds.). International Scientific Meeting Cassava Biotechnology Network (1, 1992, Cartagena de Indias, Colombia). Proceedings. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 185-189. (Working document no. 123) |
| spellingShingle | manihot esculenta anther culture embryo culture methods culture media cultivo de anteras cultivo de embriones métodos medio de cultivo Cataño C, ML Mornan, K Plazas, J Roca, W. Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title | Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title_full | Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title_fullStr | Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title_full_unstemmed | Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title_short | Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| title_sort | development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos |
| topic | manihot esculenta anther culture embryo culture methods culture media cultivo de anteras cultivo de embriones métodos medio de cultivo |
| url | https://hdl.handle.net/10568/55689 |
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