Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz)
Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were ev...
| Main Authors: | , , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
International Center for Tropical Agriculture
1993
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/55686 |
| _version_ | 1855520798521950208 |
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| author | Sarria, Rodrigo A. Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, W. |
| author_browse | Hershey, Clair H. Ocampo Nahar, César H. Ramírez, H. Roca, W. Sarria, Rodrigo A. |
| author_facet | Sarria, Rodrigo A. Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, W. |
| author_sort | Sarria, Rodrigo A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were evaluated among the segregating populations. Est-1 locus has five multiple alleles, including one null allele, and behaves as monomer, with a diploid inheritance pattern with only two alleles presenting each individual. These data were confirmed by esterase characterization of CIAT cassava germplasm bank accessions; a total of 11 different phenotypes representing 15 different genotypes for this locus were demonstrated. Got-1 locus comprises three allels with diploid inheritance. This work provides evidence for a diploid inheritance pattern of the two isozyme loci supporting the allotetraploid nature of cassava. Many other regions of the zimograms are still to be elucidated. This information will be valuable to interpret studies on isozyme fingerprinting of cassava genetic diversity. |
| format | Conference Paper |
| id | CGSpace55686 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace556862024-01-09T09:48:23Z Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) Sarria, Rodrigo A. Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, W. manihot esculenta isoenzymes hybridization heritability genes loci isoenzimas hibridación heredabilidad Genetic studies of two isozyme loci have been carried out in root tips of eight cassava F1 progenies using polyacrylamide gel electrophoresis. Two loci, esterase-1 (East-1), the most distant cationic region, and glutamate oxaloacetate transaminase-1 (Got-1), the most proximal anionic region, were evaluated among the segregating populations. Est-1 locus has five multiple alleles, including one null allele, and behaves as monomer, with a diploid inheritance pattern with only two alleles presenting each individual. These data were confirmed by esterase characterization of CIAT cassava germplasm bank accessions; a total of 11 different phenotypes representing 15 different genotypes for this locus were demonstrated. Got-1 locus comprises three allels with diploid inheritance. This work provides evidence for a diploid inheritance pattern of the two isozyme loci supporting the allotetraploid nature of cassava. Many other regions of the zimograms are still to be elucidated. This information will be valuable to interpret studies on isozyme fingerprinting of cassava genetic diversity. 1993 2015-01-28T14:21:41Z 2015-01-28T14:21:41Z Conference Paper https://hdl.handle.net/10568/55686 en Open Access International Center for Tropical Agriculture Sarria, Rodrigo A.; Ocampo Nahar, César Humberto; Ramírez, Hernando; Hershey, Clair H.; Roca, William M. 1993. Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz). In: Roca, William M.; Thro, Ann Marie (eds.). International Scientific Meeting Cassava Biotechnology Network (1, 1992, Cartagena de Indias, Colombia). Proceedings. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 75-80. (Working document no. 123) |
| spellingShingle | manihot esculenta isoenzymes hybridization heritability genes loci isoenzimas hibridación heredabilidad Sarria, Rodrigo A. Ocampo Nahar, César H. Ramírez, H. Hershey, Clair H. Roca, W. Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title | Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title_full | Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title_fullStr | Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title_full_unstemmed | Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title_short | Genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava (Manihot esculenta Crantz) |
| title_sort | genetics of esterase and glutamate oxaloacetate transaminase isozymes in cassava manihot esculenta crantz |
| topic | manihot esculenta isoenzymes hybridization heritability genes loci isoenzimas hibridación heredabilidad |
| url | https://hdl.handle.net/10568/55686 |
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