Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda
Nineteen microsatellite markers were used to investigate the genetic diversity, phylogenetic relationships and herd structure of Ugandan Ankole cattle. A total of 304 animals from 8 herds in Mbarara District were characterised. Across all loci, 200 alleles were observed. A high mean number of all...
| Main Authors: | , , , , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
TSAP and ILRI
2006
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| Online Access: | https://hdl.handle.net/10568/5523 |
| _version_ | 1855526806569877504 |
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| author | Kugonza, Donald R. Han Jianlin Okeyo Mwai, Ally Kiwuwa, G.H. Hanotte, Olivier H. |
| author_browse | Han Jianlin Hanotte, Olivier H. Kiwuwa, G.H. Kugonza, Donald R. Okeyo Mwai, Ally |
| author_facet | Kugonza, Donald R. Han Jianlin Okeyo Mwai, Ally Kiwuwa, G.H. Hanotte, Olivier H. |
| author_sort | Kugonza, Donald R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nineteen microsatellite markers were used to investigate the genetic diversity, phylogenetic
relationships and herd structure of Ugandan Ankole cattle. A total of 304 animals from 8 herds in
Mbarara District were characterised. Across all loci, 200 alleles were observed. A high mean
number of alleles (MNA) per locus, ranging from 5.89 to 6.79 per herd, was observed. Polymorphic
information content (PIC) ranged from 0.403 (ILSTS013) to 0.817 (ILSTS036), with an overall
mean of 0.688. The average observed heterozygosity (Ho) was highest in Kaibanda (0.727) and
lowest in Kituuha (0.648), while the expected heterozygosity (He) ranged from 0.722 (Nshaara)
to 0.664 (Kituuha). Significant deviations from Hardy-Weinberg proportions were observed on 8
loci, however, all 152 loci-herd combination tests were in equilibrium after Bonferroni correction.
FST estimates for all loci and between all herds were highly significant (P<0.001), suggesting
little if any gene flow between the herds. For all herd pairs, FST values were generally low, with
an overall mean of 0.041 + 0.08. Significant (P<0.01) inbreeding effect (FIS) was detected in the
Nasasira herd. The mean number of migrants per generation (Nm) across all herds was 3.82.
Phylogenetic analysis showed that herds from the same geographical counties grouped together.
These results illustrate at the molecular level the fairly wide genetic variation found among the
Ankole herds studied and therefore the potential for genetic improvement of these herds. |
| format | Conference Paper |
| id | CGSpace5523 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | TSAP and ILRI |
| publisherStr | TSAP and ILRI |
| record_format | dspace |
| spelling | CGSpace55232023-08-08T07:20:31Z Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda Kugonza, Donald R. Han Jianlin Okeyo Mwai, Ally Kiwuwa, G.H. Hanotte, Olivier H. Nineteen microsatellite markers were used to investigate the genetic diversity, phylogenetic relationships and herd structure of Ugandan Ankole cattle. A total of 304 animals from 8 herds in Mbarara District were characterised. Across all loci, 200 alleles were observed. A high mean number of alleles (MNA) per locus, ranging from 5.89 to 6.79 per herd, was observed. Polymorphic information content (PIC) ranged from 0.403 (ILSTS013) to 0.817 (ILSTS036), with an overall mean of 0.688. The average observed heterozygosity (Ho) was highest in Kaibanda (0.727) and lowest in Kituuha (0.648), while the expected heterozygosity (He) ranged from 0.722 (Nshaara) to 0.664 (Kituuha). Significant deviations from Hardy-Weinberg proportions were observed on 8 loci, however, all 152 loci-herd combination tests were in equilibrium after Bonferroni correction. FST estimates for all loci and between all herds were highly significant (P<0.001), suggesting little if any gene flow between the herds. For all herd pairs, FST values were generally low, with an overall mean of 0.041 + 0.08. Significant (P<0.01) inbreeding effect (FIS) was detected in the Nasasira herd. The mean number of migrants per generation (Nm) across all herds was 3.82. Phylogenetic analysis showed that herds from the same geographical counties grouped together. These results illustrate at the molecular level the fairly wide genetic variation found among the Ankole herds studied and therefore the potential for genetic improvement of these herds. 2006 2011-09-03T18:40:45Z 2011-09-03T18:40:45Z Conference Paper https://hdl.handle.net/10568/5523 en Open Access TSAP and ILRI Kugonza, D.R., Han Jianlin, Okeyo, A.M., Kiwuwa, G.H. and Hanotte, O. 2006. Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda. In: Rege, J.E.O.; Nyamu, A.M.; Sendalo, D. (eds.). 2006. The role of biotechnology in animal agriculture to address poverty in Africa: Opportunities and challenges. Proceedings of the 4th All Africa Conference on Animal Agriculture and the 31st annual meeting of Tanzania Society for Animal Production, Arusha, Tanzania, 20-24 September 2005. Dar es Salaam, Tanzania: TSAP and Nairobi, Kenya: ILRI. |
| spellingShingle | Kugonza, Donald R. Han Jianlin Okeyo Mwai, Ally Kiwuwa, G.H. Hanotte, Olivier H. Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title | Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title_full | Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title_fullStr | Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title_full_unstemmed | Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title_short | Microsatellite DNA polymorphism of the Long Horned Ankole cattle in Uganda |
| title_sort | microsatellite dna polymorphism of the long horned ankole cattle in uganda |
| url | https://hdl.handle.net/10568/5523 |
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