Metodos de conservacion in vitro del germoplasma

In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2 ba...

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Bibliographic Details
Main Authors: Roca, W., Arias, D.I., Chavez, R.
Format: Book Chapter
Language:Español
Published: International Center for Tropical Agriculture 1991
Subjects:
Online Access:https://hdl.handle.net/10568/55096
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author Roca, W.
Arias, D.I.
Chavez, R.
author_browse Arias, D.I.
Chavez, R.
Roca, W.
author_facet Roca, W.
Arias, D.I.
Chavez, R.
author_sort Roca, W.
collection Repository of Agricultural Research Outputs (CGSpace)
description In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2 basic systems of in vitro germplasm conservation are detailed, namely growth limitation up to min. rates and total suppression of growth. The 1st system controls the growth rate through factors such as temp., nutrient and growth regulator concn., and osmotic concn.; in the 2nd system, this rate is reduced through low temp. (levels below - 150 degrees C). In the case of cassava, germplasm is maintained through meristem culture, using a combination of cryogenic techniques and min. growth storage conditions. The different stages of a collobarative project of an in vitro active gene bank between CIAT and the International Board for Plant Genetic Resources are indicated; this project provides standards for the adoption of in vitro cassava conservation by national institutions in tropical areas or for extrapolating these standards to other crops propagated vegetatively. (CIAT)
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spelling CGSpace550962024-01-09T09:48:34Z Metodos de conservacion in vitro del germoplasma Roca, W. Arias, D.I. Chavez, R. manihot esculenta germplasm conservation tissue culture cryopreservation starch crops conservación del germoplasma cultivo de tejidos criopreservacion In vitro conservation strategies are reviewed, with special reference to in vitro active and basic gene banks; likewise, important aspects that should be considered for in vitro conservation, such as regeneration, viability, genetic stability, and information systematization, are discussed. The 2 basic systems of in vitro germplasm conservation are detailed, namely growth limitation up to min. rates and total suppression of growth. The 1st system controls the growth rate through factors such as temp., nutrient and growth regulator concn., and osmotic concn.; in the 2nd system, this rate is reduced through low temp. (levels below - 150 degrees C). In the case of cassava, germplasm is maintained through meristem culture, using a combination of cryogenic techniques and min. growth storage conditions. The different stages of a collobarative project of an in vitro active gene bank between CIAT and the International Board for Plant Genetic Resources are indicated; this project provides standards for the adoption of in vitro cassava conservation by national institutions in tropical areas or for extrapolating these standards to other crops propagated vegetatively. (CIAT) 1991 2015-01-28T14:17:25Z 2015-01-28T14:17:25Z Book Chapter https://hdl.handle.net/10568/55096 es Open Access International Center for Tropical Agriculture ROCA, W.M.; ARIAS, D.I.; CHAVEZ, R. 1991. Metodos de conservacion in vitro del germoplasma. In: Roca, William M.; Mroginski, Luis A. (eds.). Cultivo de tejidos en la agricultura: Fundamentos y aplicaciones. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 697-713.
spellingShingle manihot esculenta
germplasm conservation
tissue culture
cryopreservation
starch crops
conservación del germoplasma
cultivo de tejidos
criopreservacion
Roca, W.
Arias, D.I.
Chavez, R.
Metodos de conservacion in vitro del germoplasma
title Metodos de conservacion in vitro del germoplasma
title_full Metodos de conservacion in vitro del germoplasma
title_fullStr Metodos de conservacion in vitro del germoplasma
title_full_unstemmed Metodos de conservacion in vitro del germoplasma
title_short Metodos de conservacion in vitro del germoplasma
title_sort metodos de conservacion in vitro del germoplasma
topic manihot esculenta
germplasm conservation
tissue culture
cryopreservation
starch crops
conservación del germoplasma
cultivo de tejidos
criopreservacion
url https://hdl.handle.net/10568/55096
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AT ariasdi metodosdeconservacioninvitrodelgermoplasma
AT chavezr metodosdeconservacioninvitrodelgermoplasma