Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz)
Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, and g...
| Autores principales: | , , , , |
|---|---|
| Formato: | Capítulo de libro |
| Lenguaje: | Español |
| Publicado: |
International Center for Tropical Agriculture
1991
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/55095 |
| _version_ | 1855527073528938496 |
|---|---|
| author | Roca, W. Nolt, Barry L. Mafla, G. Roa E, Julio César Reyes, R. |
| author_browse | Mafla, G. Nolt, Barry L. Reyes, R. Roa E, Julio César Roca, W. |
| author_facet | Roca, W. Nolt, Barry L. Mafla, G. Roa E, Julio César Reyes, R. |
| author_sort | Roca, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, and grafting trials. Clones submitted to pathogenic tests can be maintained in vitro, avoiding the recontamination with pathogenic organisms. The interrelations existing between the different forms of in vitro conservation of cassava germplasm and the elimination of diseases, micropropagation, distribution, and traditional conservation through seed and field plantations (basic vs. active collections) are indicated. The use of in vitro clonal propagation in cassava and the combination of different techniques to satisfy all multiplication needs are discussed, namely micropropagation through single-node cultures, multiple shoot culture, and somatic embryogenesis. Several examples are given that illustrate that rehabilitation of regional cassava cv. is a strategy to produce cassava in the poorer regions of the tropics in the short term. (CIAT) |
| format | Book Chapter |
| id | CGSpace55095 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace550952024-01-09T09:48:43Z Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) Roca, W. Nolt, Barry L. Mafla, G. Roa E, Julio César Reyes, R. manihot esculenta virus inhibition tissue culture apical meristems heat therapy elisa germplasm propagation cultivation disease control pest control plant tissues starch crops inhibicion de virus cultivo de tejidos meristemas apicales tratamiento termico germoplasma propagacion Several techniques used to eliminate viruses from cassava are described, namely heat therapy, apical tissue culture, and micropropagation. Virological tests to index cassava viruses are also indicated as follows: ELISA, dsRNA, mechanical inoculation of leaf sap in plants on Chenopodium quinoa, and grafting trials. Clones submitted to pathogenic tests can be maintained in vitro, avoiding the recontamination with pathogenic organisms. The interrelations existing between the different forms of in vitro conservation of cassava germplasm and the elimination of diseases, micropropagation, distribution, and traditional conservation through seed and field plantations (basic vs. active collections) are indicated. The use of in vitro clonal propagation in cassava and the combination of different techniques to satisfy all multiplication needs are discussed, namely micropropagation through single-node cultures, multiple shoot culture, and somatic embryogenesis. Several examples are given that illustrate that rehabilitation of regional cassava cv. is a strategy to produce cassava in the poorer regions of the tropics in the short term. (CIAT) 1991 2015-01-28T14:17:25Z 2015-01-28T14:17:25Z Book Chapter https://hdl.handle.net/10568/55095 es Open Access International Center for Tropical Agriculture ROCA, W.M.; NOLT, B.; MAFLA, G.; ROA, J.; REYES, R. 1991. Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz). In: Roca, William M.; Mroginski, Luis A. (eds.). Cultivo de tejidos en la agricultura: Fundamentos y aplicaciones. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 403-420. |
| spellingShingle | manihot esculenta virus inhibition tissue culture apical meristems heat therapy elisa germplasm propagation cultivation disease control pest control plant tissues starch crops inhibicion de virus cultivo de tejidos meristemas apicales tratamiento termico germoplasma propagacion Roca, W. Nolt, Barry L. Mafla, G. Roa E, Julio César Reyes, R. Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title | Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title_full | Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title_fullStr | Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title_full_unstemmed | Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title_short | Eliminacion de virus y propagacion de clones en la yuca (Manihot esculenta Crantz) |
| title_sort | eliminacion de virus y propagacion de clones en la yuca manihot esculenta crantz |
| topic | manihot esculenta virus inhibition tissue culture apical meristems heat therapy elisa germplasm propagation cultivation disease control pest control plant tissues starch crops inhibicion de virus cultivo de tejidos meristemas apicales tratamiento termico germoplasma propagacion |
| url | https://hdl.handle.net/10568/55095 |
| work_keys_str_mv | AT rocaw eliminaciondevirusypropagaciondeclonesenlayucamanihotesculentacrantz AT noltbarryl eliminaciondevirusypropagaciondeclonesenlayucamanihotesculentacrantz AT maflag eliminaciondevirusypropagaciondeclonesenlayucamanihotesculentacrantz AT roaejuliocesar eliminaciondevirusypropagaciondeclonesenlayucamanihotesculentacrantz AT reyesr eliminaciondevirusypropagaciondeclonesenlayucamanihotesculentacrantz |