Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam

Nhue River is water spinach, consuming those raw spinach are at high risk of exposure to microbial. The purpose of this study was to assess the health risks of consuming raw water spinach contaminated by Coliforms, G. lamblia and C. parvum in Nhue River, Hanam Province. A total of 36 water spinach s...

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Main Authors: Kieu Thanh Truc, Luu Quoc Toan, Dang Xuan Sinh, Phuc Pham Duc, Tran Thi Tuyet Hanh, Nguyen Hung Long, Hung Nguyen-Viet
Format: Journal Article
Language:Inglés
Published: 2014
Online Access:https://hdl.handle.net/10568/53936
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author Kieu Thanh Truc
Luu Quoc Toan
Dang Xuan Sinh
Phuc Pham Duc
Tran Thi Tuyet Hanh
Nguyen Hung Long
Hung Nguyen-Viet
author_browse Dang Xuan Sinh
Hung Nguyen-Viet
Kieu Thanh Truc
Luu Quoc Toan
Nguyen Hung Long
Phuc Pham Duc
Tran Thi Tuyet Hanh
author_facet Kieu Thanh Truc
Luu Quoc Toan
Dang Xuan Sinh
Phuc Pham Duc
Tran Thi Tuyet Hanh
Nguyen Hung Long
Hung Nguyen-Viet
author_sort Kieu Thanh Truc
collection Repository of Agricultural Research Outputs (CGSpace)
description Nhue River is water spinach, consuming those raw spinach are at high risk of exposure to microbial. The purpose of this study was to assess the health risks of consuming raw water spinach contaminated by Coliforms, G. lamblia and C. parvum in Nhue River, Hanam Province. A total of 36 water spinach samples and nine water samples were collected for three types of pathogens analyses using Most Probable Number (MPN) and Immuno-fluorescent Antibodies (IFA) methods. We have assumed that 49% of fecal coliforms were E. coli and that 8% of E. coli were pathogenic. To simulate the impact of washing vegetable to remove pathogens from vegetable, water spinach were washed one, two and three times with soak in tap water. The results showed that E. coli O157:H7 in water spinach without washing, one time washing, two and three times washing reduced from 3.23 ± 1.64 to 1.42 ± 1.77 CFU/g. An average number of E. coli O157:H7 in river water were 4.77 log CFU/100ml. The mean amount of raw spinach consumption was estimated at 40.22g/person/meal, mean frequency of raw spinach consumption was 1.39 meals/person/year. The diarrhea risk associated with E. coli O157:H7 when consuming raw water spinach washed three times was 0.25; the diarrhea risks due to G. lamblia and C. parvum were 0 and 0.23, respectively. This study shows the high contamination of microbial in raw water spinach grown on Nhue River. Appropriate practices for raw water spinach preparation and consumption at the household to reduce or prevent infection risk and diarrhea risk by microbial is recommended.
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spelling CGSpace539362023-02-15T10:17:40Z Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam Kieu Thanh Truc Luu Quoc Toan Dang Xuan Sinh Phuc Pham Duc Tran Thi Tuyet Hanh Nguyen Hung Long Hung Nguyen-Viet Nhue River is water spinach, consuming those raw spinach are at high risk of exposure to microbial. The purpose of this study was to assess the health risks of consuming raw water spinach contaminated by Coliforms, G. lamblia and C. parvum in Nhue River, Hanam Province. A total of 36 water spinach samples and nine water samples were collected for three types of pathogens analyses using Most Probable Number (MPN) and Immuno-fluorescent Antibodies (IFA) methods. We have assumed that 49% of fecal coliforms were E. coli and that 8% of E. coli were pathogenic. To simulate the impact of washing vegetable to remove pathogens from vegetable, water spinach were washed one, two and three times with soak in tap water. The results showed that E. coli O157:H7 in water spinach without washing, one time washing, two and three times washing reduced from 3.23 ± 1.64 to 1.42 ± 1.77 CFU/g. An average number of E. coli O157:H7 in river water were 4.77 log CFU/100ml. The mean amount of raw spinach consumption was estimated at 40.22g/person/meal, mean frequency of raw spinach consumption was 1.39 meals/person/year. The diarrhea risk associated with E. coli O157:H7 when consuming raw water spinach washed three times was 0.25; the diarrhea risks due to G. lamblia and C. parvum were 0 and 0.23, respectively. This study shows the high contamination of microbial in raw water spinach grown on Nhue River. Appropriate practices for raw water spinach preparation and consumption at the household to reduce or prevent infection risk and diarrhea risk by microbial is recommended. 2014 2015-01-28T09:19:52Z 2015-01-28T09:19:52Z Journal Article https://hdl.handle.net/10568/53936 en Limited Access Kieu Thanh Truc, Luu Quoc Toan, Dang Xuan Sinh, Pham Duc Phuc, Tran Thi Tuyet Hanh, Nguyen Hung Long and Hung Nguyen-Viet. 2014. Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam. Journal of Practical Medicine 5(933-934): 237-240.
spellingShingle Kieu Thanh Truc
Luu Quoc Toan
Dang Xuan Sinh
Phuc Pham Duc
Tran Thi Tuyet Hanh
Nguyen Hung Long
Hung Nguyen-Viet
Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title_full Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title_fullStr Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title_full_unstemmed Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title_short Risk assessment of E. coli, G. lamblia and C. parvum in raw spinach grown on Nhue River in Hanam
title_sort risk assessment of e coli g lamblia and c parvum in raw spinach grown on nhue river in hanam
url https://hdl.handle.net/10568/53936
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