Reliable molecular identification of nine tropical whitefly species
The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming o...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/51662 |
| _version_ | 1855518911207833600 |
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| author | Ovalle Rivera, Tatiana M. Parsa, Soroush Hernández M., María del Pilar Becerra López Lavelle, Luis Augusto |
| author_browse | Becerra López Lavelle, Luis Augusto Hernández M., María del Pilar Ovalle Rivera, Tatiana M. Parsa, Soroush |
| author_facet | Ovalle Rivera, Tatiana M. Parsa, Soroush Hernández M., María del Pilar Becerra López Lavelle, Luis Augusto |
| author_sort | Ovalle Rivera, Tatiana M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (COI) gene is often used for mitochondrial haplotype identification that can be associated with specific species. Our objective was to compare morphometric traits against DNA barcode sequences to develop and implement a diagnostic molecular kit based on a RFLP‐PCR method using the COI gene for the rapid identification of whiteflies. This study will allow for the rapid diagnosis of the diverse community of whiteflies attacking plants of economic interest in Colombia. It also provides access to the COI sequence that can be used to develop predator conservation techniques by establishing which predators have a trophic linkage with the focal whitefly pest species. |
| format | Journal Article |
| id | CGSpace51662 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace516622025-03-13T09:44:03Z Reliable molecular identification of nine tropical whitefly species Ovalle Rivera, Tatiana M. Parsa, Soroush Hernández M., María del Pilar Becerra López Lavelle, Luis Augusto pest insects pest control insect control hemiptera dna insectos dañinos control de plagas ecology The identification of whitefly species in adult stage is problematic. Morphological differentiation of pupae is one of the better methods for determining identity of species, but it may vary depending on the host plant on which they develop which can lead to misidentifications and erroneous naming of new species. Polymerase chain reaction (PCR) fragment amplified from the mitochondrial cytochrome oxidase I (COI) gene is often used for mitochondrial haplotype identification that can be associated with specific species. Our objective was to compare morphometric traits against DNA barcode sequences to develop and implement a diagnostic molecular kit based on a RFLP‐PCR method using the COI gene for the rapid identification of whiteflies. This study will allow for the rapid diagnosis of the diverse community of whiteflies attacking plants of economic interest in Colombia. It also provides access to the COI sequence that can be used to develop predator conservation techniques by establishing which predators have a trophic linkage with the focal whitefly pest species. 2014-10 2014-11-29T05:29:09Z 2014-11-29T05:29:09Z Journal Article https://hdl.handle.net/10568/51662 en Open Access Wiley Ovalle, Tatiana M.; Parsa, Soroush; Hernández, Maria P.; Becerra López Lavelle, Luis Augusto. 2014. Reliable molecular identification of nine tropical whitefly species. Ecology and Evolution 4(9): 3778-3787. |
| spellingShingle | pest insects pest control insect control hemiptera dna insectos dañinos control de plagas ecology Ovalle Rivera, Tatiana M. Parsa, Soroush Hernández M., María del Pilar Becerra López Lavelle, Luis Augusto Reliable molecular identification of nine tropical whitefly species |
| title | Reliable molecular identification of nine tropical whitefly species |
| title_full | Reliable molecular identification of nine tropical whitefly species |
| title_fullStr | Reliable molecular identification of nine tropical whitefly species |
| title_full_unstemmed | Reliable molecular identification of nine tropical whitefly species |
| title_short | Reliable molecular identification of nine tropical whitefly species |
| title_sort | reliable molecular identification of nine tropical whitefly species |
| topic | pest insects pest control insect control hemiptera dna insectos dañinos control de plagas ecology |
| url | https://hdl.handle.net/10568/51662 |
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