Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia
Since 1992, anthracnose of Andean blackberry (Rubus glaucus) has generated losses as high as 40% for farmers in Colombia. In this study, our goal was to characterize 240 Colletotrichum isolates from Andean blackberry in eight areas of Colombia. These isolates were evaluated according to morphologica...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Scientific Societies
2014
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/51659 |
| _version_ | 1855542101100462080 |
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| author | Afanador Kafury, Lucía González, Alonso Gañan, Lederson Mejía, Juan Fernando Cardona, Nadya Álvarez, Elizabeth |
| author_browse | Afanador Kafury, Lucía Cardona, Nadya Gañan, Lederson González, Alonso Mejía, Juan Fernando Álvarez, Elizabeth |
| author_facet | Afanador Kafury, Lucía González, Alonso Gañan, Lederson Mejía, Juan Fernando Cardona, Nadya Álvarez, Elizabeth |
| author_sort | Afanador Kafury, Lucía |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Since 1992, anthracnose of Andean blackberry (Rubus glaucus) has generated losses as high as 40% for farmers in Colombia. In this study, our goal was to characterize 240 Colletotrichum isolates from Andean blackberry in eight areas of Colombia. These isolates were evaluated according to morphological characteristics, sensitivity to benomyl, pathogenicity, and genetic variability. Identification of the genus Colletotrichum was achieved by using species complex-specific polymerase chain reaction primers. A multilocus phylogeny approach was used to identify isolates to the species level with sequences from the ribosomal internal transcribed spacer region and partial sequences of the actin, β-tubulin 2, calmodulin, chitin synthase 1, glutamine synthetase, and glyceraldehyde-3-phosphate dehydrogenase genes. Most of the isolates were identified as Colletotrichum gloeosporioides sensu lato, were associated with the Castilla ecotype, showed high sensitivity to benomyl, and were highly aggressive. Isolates identified as C. acutatum sensu lato were found mainly on the Thornless ecotype, were highly resistant to benomyl, and showed intermediate aggressiveness. Only three isolates were identified as C. boninense sensu lato. The species identified included C. fructicola, C. kahawae subsp. ciggaro, C. godetiae, C. karstii, C. brassicicola, and undetermined Colletotrichum spp. This study is the first report of these species associated with anthracnose in Andean blackberry. |
| format | Journal Article |
| id | CGSpace51659 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace516592025-03-13T09:45:48Z Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia Afanador Kafury, Lucía González, Alonso Gañan, Lederson Mejía, Juan Fernando Cardona, Nadya Álvarez, Elizabeth colletotrichum fungal diseases plant diseases tropical fruits anthracnosis pest control enfermedades fungosas enfermedades de las plantas frutas tropicales control de plagas Since 1992, anthracnose of Andean blackberry (Rubus glaucus) has generated losses as high as 40% for farmers in Colombia. In this study, our goal was to characterize 240 Colletotrichum isolates from Andean blackberry in eight areas of Colombia. These isolates were evaluated according to morphological characteristics, sensitivity to benomyl, pathogenicity, and genetic variability. Identification of the genus Colletotrichum was achieved by using species complex-specific polymerase chain reaction primers. A multilocus phylogeny approach was used to identify isolates to the species level with sequences from the ribosomal internal transcribed spacer region and partial sequences of the actin, β-tubulin 2, calmodulin, chitin synthase 1, glutamine synthetase, and glyceraldehyde-3-phosphate dehydrogenase genes. Most of the isolates were identified as Colletotrichum gloeosporioides sensu lato, were associated with the Castilla ecotype, showed high sensitivity to benomyl, and were highly aggressive. Isolates identified as C. acutatum sensu lato were found mainly on the Thornless ecotype, were highly resistant to benomyl, and showed intermediate aggressiveness. Only three isolates were identified as C. boninense sensu lato. The species identified included C. fructicola, C. kahawae subsp. ciggaro, C. godetiae, C. karstii, C. brassicicola, and undetermined Colletotrichum spp. This study is the first report of these species associated with anthracnose in Andean blackberry. 2014-11 2014-11-29T05:26:38Z 2014-11-29T05:26:38Z Journal Article https://hdl.handle.net/10568/51659 en Open Access Scientific Societies Afanador Kafury, Lucía; González, Alonso; Gañan, Lederson; Mejía, Juan Fernando; Cardona, Nadya; Alvarez, Elizabeth. 2014. Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia. Plant Disease 98(11): 1503-1513. |
| spellingShingle | colletotrichum fungal diseases plant diseases tropical fruits anthracnosis pest control enfermedades fungosas enfermedades de las plantas frutas tropicales control de plagas Afanador Kafury, Lucía González, Alonso Gañan, Lederson Mejía, Juan Fernando Cardona, Nadya Álvarez, Elizabeth Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title | Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title_full | Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title_fullStr | Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title_full_unstemmed | Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title_short | Characterization of the Colletotrichum species causing anthracnose in andean blackberry in Colombia |
| title_sort | characterization of the colletotrichum species causing anthracnose in andean blackberry in colombia |
| topic | colletotrichum fungal diseases plant diseases tropical fruits anthracnosis pest control enfermedades fungosas enfermedades de las plantas frutas tropicales control de plagas |
| url | https://hdl.handle.net/10568/51659 |
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