Identification of Cassava MicroRNAs under abiotic stress
The study of microRNAs (miRNAs) in plants has gained significant attention in recent years due to their regulatory role during development and in response to biotic and abiotic stresses. Although cassava (Manihot esculentaCrantz) is tolerant to drought and other adverse conditions, most cassava miRN...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Hindawi Limited
2013
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/51415 |
| _version_ | 1855537926265372672 |
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| author | Ballén-Taborda, Carolina Plata, Germán Ayling, Sarah Rodríguez Zapata, Fausto Becerra López Lavelle, Luis Augusto Duitama, Jorge Tohme, Joseph M. |
| author_browse | Ayling, Sarah Ballén-Taborda, Carolina Becerra López Lavelle, Luis Augusto Duitama, Jorge Plata, Germán Rodríguez Zapata, Fausto Tohme, Joseph M. |
| author_facet | Ballén-Taborda, Carolina Plata, Germán Ayling, Sarah Rodríguez Zapata, Fausto Becerra López Lavelle, Luis Augusto Duitama, Jorge Tohme, Joseph M. |
| author_sort | Ballén-Taborda, Carolina |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The study of microRNAs (miRNAs) in plants has gained significant attention in recent years due to their regulatory role during development and in response to biotic and abiotic stresses. Although cassava (Manihot esculentaCrantz) is tolerant to drought and other adverse conditions, most cassava miRNAs have been predicted using bioinformatics alone or through sequencing of plants challenged by biotic stress. Here, we use high-throughput sequencing and different bioinformatics methods to identify potential cassava miRNAs expressed in different tissues subject to heat and drought conditions. We identified 60 miRNAs conserved in other plant species and 821 potential cassava-specific miRNAs. We also predicted 134 and 1002 potential target genes for these two sets of sequences. Using real time PCR, we verified the condition-specific expression of 5 cassava small RNAs relative to a non-stress control. We also found, using publicly available expression data, a significantly lower expression of the predicted target genes of conserved and nonconserved miRNAs under drought stress compared to other cassava genes. Gene Ontology enrichment analysis along with condition specific expression of predicted miRNA targets, allowed us to identify several interesting miRNAs which may play a role in stress-induced posttranscriptional regulation in cassava and other plants. |
| format | Journal Article |
| id | CGSpace51415 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Hindawi Limited |
| publisherStr | Hindawi Limited |
| record_format | dspace |
| spelling | CGSpace514152024-08-27T10:37:02Z Identification of Cassava MicroRNAs under abiotic stress Ballén-Taborda, Carolina Plata, Germán Ayling, Sarah Rodríguez Zapata, Fausto Becerra López Lavelle, Luis Augusto Duitama, Jorge Tohme, Joseph M. manihot esculenta in vitro culture rna gene expression abiotic stress drought cultivo in vitro The study of microRNAs (miRNAs) in plants has gained significant attention in recent years due to their regulatory role during development and in response to biotic and abiotic stresses. Although cassava (Manihot esculentaCrantz) is tolerant to drought and other adverse conditions, most cassava miRNAs have been predicted using bioinformatics alone or through sequencing of plants challenged by biotic stress. Here, we use high-throughput sequencing and different bioinformatics methods to identify potential cassava miRNAs expressed in different tissues subject to heat and drought conditions. We identified 60 miRNAs conserved in other plant species and 821 potential cassava-specific miRNAs. We also predicted 134 and 1002 potential target genes for these two sets of sequences. Using real time PCR, we verified the condition-specific expression of 5 cassava small RNAs relative to a non-stress control. We also found, using publicly available expression data, a significantly lower expression of the predicted target genes of conserved and nonconserved miRNAs under drought stress compared to other cassava genes. Gene Ontology enrichment analysis along with condition specific expression of predicted miRNA targets, allowed us to identify several interesting miRNAs which may play a role in stress-induced posttranscriptional regulation in cassava and other plants. 2013 2014-11-12T13:42:16Z 2014-11-12T13:42:16Z Journal Article https://hdl.handle.net/10568/51415 en Open Access Hindawi Limited Ballon-Taborda, Carolina; Plata, German; Ayling, Sarah; Rodriguez-Zapata, Fausto; Becerra Lopez-Lavalle, Luis Augusto; Duitama, Jorge; Tohme, Joseph M. 2013. Identification of Cassava MicroRNAs under abiotic stress. International Journal of Genomics. Published online. |
| spellingShingle | manihot esculenta in vitro culture rna gene expression abiotic stress drought cultivo in vitro Ballén-Taborda, Carolina Plata, Germán Ayling, Sarah Rodríguez Zapata, Fausto Becerra López Lavelle, Luis Augusto Duitama, Jorge Tohme, Joseph M. Identification of Cassava MicroRNAs under abiotic stress |
| title | Identification of Cassava MicroRNAs under abiotic stress |
| title_full | Identification of Cassava MicroRNAs under abiotic stress |
| title_fullStr | Identification of Cassava MicroRNAs under abiotic stress |
| title_full_unstemmed | Identification of Cassava MicroRNAs under abiotic stress |
| title_short | Identification of Cassava MicroRNAs under abiotic stress |
| title_sort | identification of cassava micrornas under abiotic stress |
| topic | manihot esculenta in vitro culture rna gene expression abiotic stress drought cultivo in vitro |
| url | https://hdl.handle.net/10568/51415 |
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