Methods for detection of drug resistance in trypanosomes - new developments?

Characterising the drug susceptibility of trypanosomes that are pathogenic for domestic livestock has for many years been carried out either in vivo, using large or small animals, or by using various in vitro cultivation methodologies (Peregrine, 1994). Thus, adaptation to, and multiplication of fie...

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Main Authors: Peregrine, A.S., Wells, C.W., Wilkes, J.M.
Format: Conference Paper
Language:Inglés
Published: European Union 2000
Subjects:
Online Access:https://hdl.handle.net/10568/50845
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author Peregrine, A.S.
Wells, C.W.
Wilkes, J.M.
author_browse Peregrine, A.S.
Wells, C.W.
Wilkes, J.M.
author_facet Peregrine, A.S.
Wells, C.W.
Wilkes, J.M.
author_sort Peregrine, A.S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Characterising the drug susceptibility of trypanosomes that are pathogenic for domestic livestock has for many years been carried out either in vivo, using large or small animals, or by using various in vitro cultivation methodologies (Peregrine, 1994). Thus, adaptation to, and multiplication of field isolates in, an experimental environment is often a requirement for obtaining drug sensitivity data. However, many in vivo and in vitro systems suffer from a number of shortcomings: (a) field isolates of trypanosomes frequently fail to establish or grow poorly, (b) it can often take many weeks or months to characterise a trypanosome population, and (c) it is generally not practical to screen a large number of trypanosome populations. As a result, for epidemiology studies, there is a need to develop new techniques that will rapidly characterise the drug sensitivity of large numbers of trypanosome isolates, immediately upon collection from domestic livestock. This review summarises three recent areas of research on resistance to isometamidium (Samorin\AE, Trypamidium) in trypanosomes and indicates whether or not the results may lead to the Development of such diagnostic tests.
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spelling CGSpace508452024-01-08T18:54:14Z Methods for detection of drug resistance in trypanosomes - new developments? Peregrine, A.S. Wells, C.W. Wilkes, J.M. trypanosomes (phytomonas) drug resistance livestock Characterising the drug susceptibility of trypanosomes that are pathogenic for domestic livestock has for many years been carried out either in vivo, using large or small animals, or by using various in vitro cultivation methodologies (Peregrine, 1994). Thus, adaptation to, and multiplication of field isolates in, an experimental environment is often a requirement for obtaining drug sensitivity data. However, many in vivo and in vitro systems suffer from a number of shortcomings: (a) field isolates of trypanosomes frequently fail to establish or grow poorly, (b) it can often take many weeks or months to characterise a trypanosome population, and (c) it is generally not practical to screen a large number of trypanosome populations. As a result, for epidemiology studies, there is a need to develop new techniques that will rapidly characterise the drug sensitivity of large numbers of trypanosome isolates, immediately upon collection from domestic livestock. This review summarises three recent areas of research on resistance to isometamidium (Samorin\AE, Trypamidium) in trypanosomes and indicates whether or not the results may lead to the Development of such diagnostic tests. 2000 2014-10-31T06:21:44Z 2014-10-31T06:21:44Z Conference Paper https://hdl.handle.net/10568/50845 en Limited Access European Union
spellingShingle trypanosomes (phytomonas)
drug resistance
livestock
Peregrine, A.S.
Wells, C.W.
Wilkes, J.M.
Methods for detection of drug resistance in trypanosomes - new developments?
title Methods for detection of drug resistance in trypanosomes - new developments?
title_full Methods for detection of drug resistance in trypanosomes - new developments?
title_fullStr Methods for detection of drug resistance in trypanosomes - new developments?
title_full_unstemmed Methods for detection of drug resistance in trypanosomes - new developments?
title_short Methods for detection of drug resistance in trypanosomes - new developments?
title_sort methods for detection of drug resistance in trypanosomes new developments
topic trypanosomes (phytomonas)
drug resistance
livestock
url https://hdl.handle.net/10568/50845
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