DNA probe- and PCR-based methods for the detection of trypanosomes
Accurate identification of an infectious organism facilitates the study of epidemiology of the disease it causes and makes possible rational evaluation of the relative success of different control strategies. Highly repetitive, tandem or interspersed DNA sequences unique to different species, type o...
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
| Publicado: |
OAU/STRC
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/50400 |
| _version_ | 1855539920161996800 |
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| author | Majiwa, Phelix A.O. |
| author_browse | Majiwa, Phelix A.O. |
| author_facet | Majiwa, Phelix A.O. |
| author_sort | Majiwa, Phelix A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Accurate identification of an infectious organism facilitates the study of epidemiology of the disease it causes and makes possible rational evaluation of the relative success of different control strategies. Highly repetitive, tandem or interspersed DNA sequences unique to different species, type or sub-type of the African trypanosomes have been cloned as recombinant plasmids for use in specific and sensitive identification of the trypanosomes. Protocols have been adapted for use with these DNA probes in the detection of trypanosomes in the blood or buffy coat samples from mammalian hosts and the saliva of live tsetse flies. The detection of parasite DNA relies upon hybridization with parasite type-specific DNA probe labelled with digoxigenin, followed by revealing the hybridized probe using anti-digoxigenin antibodies conjugated to alkaline phosphatase and the addition of enzyme substrates resulting in either visible colour or emission of light detectable by autoradiography. Combined with the polymerase chain reaction (PCR), the method detects trypanosomes in bufffy-coat samples from antigenaemic but aparasitaemic cattle, and in the saliva of live infected tsetse flies. The majority of these recombinant DNA probes are presently available from various sources. |
| format | Conference Paper |
| id | CGSpace50400 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | OAU/STRC |
| publisherStr | OAU/STRC |
| record_format | dspace |
| spelling | CGSpace504002024-03-06T10:16:43Z DNA probe- and PCR-based methods for the detection of trypanosomes Majiwa, Phelix A.O. trypanosomes (phytomonas) diagnosis methods Accurate identification of an infectious organism facilitates the study of epidemiology of the disease it causes and makes possible rational evaluation of the relative success of different control strategies. Highly repetitive, tandem or interspersed DNA sequences unique to different species, type or sub-type of the African trypanosomes have been cloned as recombinant plasmids for use in specific and sensitive identification of the trypanosomes. Protocols have been adapted for use with these DNA probes in the detection of trypanosomes in the blood or buffy coat samples from mammalian hosts and the saliva of live tsetse flies. The detection of parasite DNA relies upon hybridization with parasite type-specific DNA probe labelled with digoxigenin, followed by revealing the hybridized probe using anti-digoxigenin antibodies conjugated to alkaline phosphatase and the addition of enzyme substrates resulting in either visible colour or emission of light detectable by autoradiography. Combined with the polymerase chain reaction (PCR), the method detects trypanosomes in bufffy-coat samples from antigenaemic but aparasitaemic cattle, and in the saliva of live infected tsetse flies. The majority of these recombinant DNA probes are presently available from various sources. 1995 2014-10-31T06:09:10Z 2014-10-31T06:09:10Z Conference Paper https://hdl.handle.net/10568/50400 en Limited Access OAU/STRC |
| spellingShingle | trypanosomes (phytomonas) diagnosis methods Majiwa, Phelix A.O. DNA probe- and PCR-based methods for the detection of trypanosomes |
| title | DNA probe- and PCR-based methods for the detection of trypanosomes |
| title_full | DNA probe- and PCR-based methods for the detection of trypanosomes |
| title_fullStr | DNA probe- and PCR-based methods for the detection of trypanosomes |
| title_full_unstemmed | DNA probe- and PCR-based methods for the detection of trypanosomes |
| title_short | DNA probe- and PCR-based methods for the detection of trypanosomes |
| title_sort | dna probe and pcr based methods for the detection of trypanosomes |
| topic | trypanosomes (phytomonas) diagnosis methods |
| url | https://hdl.handle.net/10568/50400 |
| work_keys_str_mv | AT majiwaphelixao dnaprobeandpcrbasedmethodsforthedetectionoftrypanosomes |