Bovine alloreactive cytotoxic T cells

The target antigens recognized by bovine alloreactive cytotoxic T cells are discussed and compared with serologically defined bovine major histocompatibility complex (MHC) products which make up the bovine lymphocyte antigen (BoLA) system. The role of MHC products in T-cell function in other specie...

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Main Authors: Teale, A.J., Morrison, W. Ivan, Spooner, R.L., Goddeeris, Bruno M., Groocock, C.M., Stagg, D.A.
Format: Conference Paper
Language:Inglés
Published: Cambridge University Press 1986
Subjects:
Online Access:https://hdl.handle.net/10568/50237
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author Teale, A.J.
Morrison, W. Ivan
Spooner, R.L.
Goddeeris, Bruno M.
Groocock, C.M.
Stagg, D.A.
author_browse Goddeeris, Bruno M.
Groocock, C.M.
Morrison, W. Ivan
Spooner, R.L.
Stagg, D.A.
Teale, A.J.
author_facet Teale, A.J.
Morrison, W. Ivan
Spooner, R.L.
Goddeeris, Bruno M.
Groocock, C.M.
Stagg, D.A.
author_sort Teale, A.J.
collection Repository of Agricultural Research Outputs (CGSpace)
description The target antigens recognized by bovine alloreactive cytotoxic T cells are discussed and compared with serologically defined bovine major histocompatibility complex (MHC) products which make up the bovine lymphocyte antigen (BoLA) system. The role of MHC products in T-cell function in other species is reviewed with particular emphasis on the use of cytotoxic T cells in the characterization of human and murine MHC products and in the Development of cytotoxic T-cell technology in general. In the bovine system, serologically defined BoLA-A-locus products w8 and w11 are closely associated with, or identical to, the target determinants for cytotoxic T cells generated in vitro in peripheral blood mononuclear cells (PMB) of unprimed w8- and w11-negative cattle cocultured with w8/w11 PMB. There was little evidence of cytotoxicity directed against other products of the MHC. In similar studies of the specificity of cytotoxic T cells generated against PMB carrying the serologically defined specificity w6 and its subgroups, cytotoxicity could be detected with correlated with distribution of the subgroups of w6 on target cells, but not with w6 itself. The generation and specificity of cytotoxic T cells in cocultures of w8/w11 responder PMB and w8/w11 stimulator PMB were also examined. In some cases, cytotoxic T cells were generated and their target specificity suggested that products of a second cell-mediated lympholysis-controlling locus in cattle were being detected. The nature of these products is not known.
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spelling CGSpace502372024-01-17T12:58:34Z Bovine alloreactive cytotoxic T cells Teale, A.J. Morrison, W. Ivan Spooner, R.L. Goddeeris, Bruno M. Groocock, C.M. Stagg, D.A. cattle lymphocytes The target antigens recognized by bovine alloreactive cytotoxic T cells are discussed and compared with serologically defined bovine major histocompatibility complex (MHC) products which make up the bovine lymphocyte antigen (BoLA) system. The role of MHC products in T-cell function in other species is reviewed with particular emphasis on the use of cytotoxic T cells in the characterization of human and murine MHC products and in the Development of cytotoxic T-cell technology in general. In the bovine system, serologically defined BoLA-A-locus products w8 and w11 are closely associated with, or identical to, the target determinants for cytotoxic T cells generated in vitro in peripheral blood mononuclear cells (PMB) of unprimed w8- and w11-negative cattle cocultured with w8/w11 PMB. There was little evidence of cytotoxicity directed against other products of the MHC. In similar studies of the specificity of cytotoxic T cells generated against PMB carrying the serologically defined specificity w6 and its subgroups, cytotoxicity could be detected with correlated with distribution of the subgroups of w6 on target cells, but not with w6 itself. The generation and specificity of cytotoxic T cells in cocultures of w8/w11 responder PMB and w8/w11 stimulator PMB were also examined. In some cases, cytotoxic T cells were generated and their target specificity suggested that products of a second cell-mediated lympholysis-controlling locus in cattle were being detected. The nature of these products is not known. 1986 2014-10-31T06:08:58Z 2014-10-31T06:08:58Z Conference Paper https://hdl.handle.net/10568/50237 en Limited Access Cambridge University Press
spellingShingle cattle
lymphocytes
Teale, A.J.
Morrison, W. Ivan
Spooner, R.L.
Goddeeris, Bruno M.
Groocock, C.M.
Stagg, D.A.
Bovine alloreactive cytotoxic T cells
title Bovine alloreactive cytotoxic T cells
title_full Bovine alloreactive cytotoxic T cells
title_fullStr Bovine alloreactive cytotoxic T cells
title_full_unstemmed Bovine alloreactive cytotoxic T cells
title_short Bovine alloreactive cytotoxic T cells
title_sort bovine alloreactive cytotoxic t cells
topic cattle
lymphocytes
url https://hdl.handle.net/10568/50237
work_keys_str_mv AT tealeaj bovinealloreactivecytotoxictcells
AT morrisonwivan bovinealloreactivecytotoxictcells
AT spoonerrl bovinealloreactivecytotoxictcells
AT goddeerisbrunom bovinealloreactivecytotoxictcells
AT groocockcm bovinealloreactivecytotoxictcells
AT staggda bovinealloreactivecytotoxictcells