Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense
In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic tr...
| Main Authors: | , |
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| Format: | Conference Paper |
| Language: | Inglés |
| Published: |
European Union
2000
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/50133 |
| _version_ | 1855513160292761600 |
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| author | Konde, V. Majiwa, Phelix A.O. |
| author_browse | Konde, V. Majiwa, Phelix A.O. |
| author_facet | Konde, V. Majiwa, Phelix A.O. |
| author_sort | Konde, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic trypanosome clones, the isometamidium chloride sensitive T. congolense ILI 180 (Nantulya et al., 1984) and its resistant derivative, IL3343 (Peregrine et al., 1997). Three major categories of genes were found: (i) those transcribed only in the trypanosome clone sensitive to isometamidium chloride, (ii) those transcribed more abundantly in only one of the two isogenic trypanosome clones, and (iii) those that are transcribed at approximately the same level, but which detect highly polymorphic loci in the isogenic trypanosome clones. |
| format | Conference Paper |
| id | CGSpace50133 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | European Union |
| publisherStr | European Union |
| record_format | dspace |
| spelling | CGSpace501332024-03-06T10:16:43Z Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense Konde, V. Majiwa, Phelix A.O. trypanosoma congolense genes drug resistance In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic trypanosome clones, the isometamidium chloride sensitive T. congolense ILI 180 (Nantulya et al., 1984) and its resistant derivative, IL3343 (Peregrine et al., 1997). Three major categories of genes were found: (i) those transcribed only in the trypanosome clone sensitive to isometamidium chloride, (ii) those transcribed more abundantly in only one of the two isogenic trypanosome clones, and (iii) those that are transcribed at approximately the same level, but which detect highly polymorphic loci in the isogenic trypanosome clones. 2000 2014-10-31T06:08:50Z 2014-10-31T06:08:50Z Conference Paper https://hdl.handle.net/10568/50133 en Limited Access European Union |
| spellingShingle | trypanosoma congolense genes drug resistance Konde, V. Majiwa, Phelix A.O. Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title | Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title_full | Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title_fullStr | Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title_full_unstemmed | Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title_short | Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense |
| title_sort | alterations in gene transcription patterns associated with the development of isometamidium resistance in trypanosoma congolense |
| topic | trypanosoma congolense genes drug resistance |
| url | https://hdl.handle.net/10568/50133 |
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