Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses
Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been ide...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/50 |
| _version_ | 1855519229056385024 |
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| author | Tinega, A.N. Pelle, Roger Kang'a, S. Gicheru, M.M. Taracha, E.L.N. Nene, Vishvanath M. Graham, S.P. |
| author_browse | Gicheru, M.M. Graham, S.P. Kang'a, S. Nene, Vishvanath M. Pelle, Roger Taracha, E.L.N. Tinega, A.N. |
| author_facet | Tinega, A.N. Pelle, Roger Kang'a, S. Gicheru, M.M. Taracha, E.L.N. Nene, Vishvanath M. Graham, S.P. |
| author_sort | Tinega, A.N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-γ ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo. |
| format | Journal Article |
| id | CGSpace50 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace502024-05-01T08:15:57Z Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses Tinega, A.N. Pelle, Roger Kang'a, S. Gicheru, M.M. Taracha, E.L.N. Nene, Vishvanath M. Graham, S.P. theileria parva proteins peptides cells immunology Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-γ ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo. 2009-07 2009-11-02T04:36:13Z 2009-11-02T04:36:13Z Journal Article https://hdl.handle.net/10568/50 en Limited Access Elsevier Tinega,, A.N.; Pellé, R..; Kang’a, S.; Gicheru,, M.M.; Taracha, E.L.N.; Nene, V.; Graham, S.P. 2009. Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses. Veterinary Immunology and Immunopathology (The Netherlands). v. 130(1-2). p. 107-113. |
| spellingShingle | theileria parva proteins peptides cells immunology Tinega, A.N. Pelle, Roger Kang'a, S. Gicheru, M.M. Taracha, E.L.N. Nene, Vishvanath M. Graham, S.P. Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title | Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title_full | Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title_fullStr | Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title_full_unstemmed | Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title_short | Fusion of a cell penetrating peptide from HIV-1 TAT to the Theileria parva antigen Tp2 enhances the stimulation of bovine CD8+ T cell responses |
| title_sort | fusion of a cell penetrating peptide from hiv 1 tat to the theileria parva antigen tp2 enhances the stimulation of bovine cd8 t cell responses |
| topic | theileria parva proteins peptides cells immunology |
| url | https://hdl.handle.net/10568/50 |
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