Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility
African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach can...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2009
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/45 |
| _version_ | 1855528148489207808 |
|---|---|
| author | O'Gorman, G.M. Park, S. de Hill, E.W. Meade, K.G. Coussens, Paul M. Agaba, Morris Naessens, Jan Kemp, Stephen J. MacHugh, David E. |
| author_browse | Agaba, Morris Coussens, Paul M. Hill, E.W. Kemp, Stephen J. MacHugh, David E. Meade, K.G. Naessens, Jan O'Gorman, G.M. Park, S. de |
| author_facet | O'Gorman, G.M. Park, S. de Hill, E.W. Meade, K.G. Coussens, Paul M. Agaba, Morris Naessens, Jan Kemp, Stephen J. MacHugh, David E. |
| author_sort | O'Gorman, G.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach can be used to identify temporal changes in host peripheral blood mononuclear cell (PBMC) gene expression due to disease progression. We also show that major gene expression differences exist between cattle from trypanotolerant and trypanosusceptible breeds. Using bovine long oligonucleotide microarrays and real time quantitative reverse transcription PCR (qRT-PCR) validation we analysed PBMC gene expression in naïve trypanotolerant and trypanosusceptible cattle experimentally challenged with Trypanosoma congolense across a 34-day infection time course. |
| format | Journal Article |
| id | CGSpace45 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace452024-05-01T08:17:51Z Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility O'Gorman, G.M. Park, S. de Hill, E.W. Meade, K.G. Coussens, Paul M. Agaba, Morris Naessens, Jan Kemp, Stephen J. MacHugh, David E. trypanosoma congolense trypanosomiasis experimentation genes African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach can be used to identify temporal changes in host peripheral blood mononuclear cell (PBMC) gene expression due to disease progression. We also show that major gene expression differences exist between cattle from trypanotolerant and trypanosusceptible breeds. Using bovine long oligonucleotide microarrays and real time quantitative reverse transcription PCR (qRT-PCR) validation we analysed PBMC gene expression in naïve trypanotolerant and trypanosusceptible cattle experimentally challenged with Trypanosoma congolense across a 34-day infection time course. 2009-12 2009-11-01T09:11:47Z 2009-11-01T09:11:47Z Journal Article https://hdl.handle.net/10568/45 en Open Access Springer O'Gorman, G.; Park, S. de; Hill, E.W.; Meade, K.G.; Coussens, P.M.; Agaba, M.; Naessens, J.; Kemp, S.J.; MacHugh, D.E. 2009. Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility. BMC Genomics. v. 10. p. 207. |
| spellingShingle | trypanosoma congolense trypanosomiasis experimentation genes O'Gorman, G.M. Park, S. de Hill, E.W. Meade, K.G. Coussens, Paul M. Agaba, Morris Naessens, Jan Kemp, Stephen J. MacHugh, David E. Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title | Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title_full | Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title_fullStr | Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title_full_unstemmed | Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title_short | Transcriptional profiling of cattle infected with Trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| title_sort | transcriptional profiling of cattle infected with trypanosoma congolense highlights gene expression signatures underlying trypanotolerance and trypanosusceptibility |
| topic | trypanosoma congolense trypanosomiasis experimentation genes |
| url | https://hdl.handle.net/10568/45 |
| work_keys_str_mv | AT ogormangm transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT parksde transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT hillew transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT meadekg transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT coussenspaulm transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT agabamorris transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT naessensjan transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT kempstephenj transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility AT machughdavide transcriptionalprofilingofcattleinfectedwithtrypanosomacongolensehighlightsgeneexpressionsignaturesunderlyingtrypanotoleranceandtrypanosusceptibility |