Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya
The research covered three slaughterhouses in Nairobi and Eldoret. The objectives were to assess the prevalence of E. coli O157 serotype contaminated carcasses at dispatch, the possible cross-contamination during transportation, and knowledge, attitude and handling practices that led to increased...
| Main Authors: | , , , |
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| Format: | Poster |
| Language: | Inglés |
| Published: |
University of Nairobi
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/44923 |
| _version_ | 1855522980438736896 |
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| author | Kago, J. Kang'ethe, Erastus K. Wangoh, J. Makita, K. |
| author_browse | Kago, J. Kang'ethe, Erastus K. Makita, K. Wangoh, J. |
| author_facet | Kago, J. Kang'ethe, Erastus K. Wangoh, J. Makita, K. |
| author_sort | Kago, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The research covered three slaughterhouses in Nairobi and Eldoret. The objectives
were to assess the prevalence of E. coli O157 serotype contaminated carcasses at
dispatch, the possible cross-contamination during transportation, and knowledge, attitude
and handling practices that led to increased contamination or bacterial growth.
Randomly selected 250 beef carcasses were sampled. Swab samples from a single
carcass were obtained from three sites during loading and offloading of meat to carriers.
A total of 1500 samples were obtained. E. coli O157 serotype was isolated, and
purified using sorbital MacConkey, MacConkey and nutrient agar. Serotyping was by
card agglutination test. Oxoid verotoxin test kit was used to test for verotoxin (VT1
and VT2) production. Carrier environment was monitored. Knowledge, attitude and
practices of meat transporters were assessed through a semi structured questionnaire
and observations. Probability of contamination was modeled and run through Monte
Carlo simulation using winBUGS®. Prevalence and data from the questionnaire were
analysed using SPSS Ver.17.
The contamination prevalence at offloading was significantly higher compared to
loading (p = 0.05). The probability of obtaining an E. coli O157 serotype contaminated
carcass at Dagoretti, Limuru and Eldoret, respectively, was 14, 16 and 19 at
loading and 31, 39 and 66 at offloading per 1000 carcasses handled. The temperature
in the meat carrier significantly increased (p = 0.004) during transportation between
loading and offloading. The average time taken to transport the meat from the slaughterhouses
to the butchery was 65 minutes.
About 14 (43.8 %) of the meat transporters had worked in the meat industry for at
least 5 years and almost an equal number 13 (40.4%) had had formal training on meat
hygiene. About 53% of meat transporters claimed to wash hands regularly with cold
water and soap. Meat carriers were cleaned at the river or in a car wash with cold water
and soap only. Carcasses were loaded on the shoulders of the transportation personnel
and placed on the floor of the carriers or heaped on top of other carcasses. Offloading
at the butchery was done by the same person with no change over of clothes that could
be soiled with blood. |
| format | Poster |
| id | CGSpace44923 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | University of Nairobi |
| publisherStr | University of Nairobi |
| record_format | dspace |
| spelling | CGSpace449232023-02-15T07:59:50Z Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya Kago, J. Kang'ethe, Erastus K. Wangoh, J. Makita, K. health animal products zoonoses The research covered three slaughterhouses in Nairobi and Eldoret. The objectives were to assess the prevalence of E. coli O157 serotype contaminated carcasses at dispatch, the possible cross-contamination during transportation, and knowledge, attitude and handling practices that led to increased contamination or bacterial growth. Randomly selected 250 beef carcasses were sampled. Swab samples from a single carcass were obtained from three sites during loading and offloading of meat to carriers. A total of 1500 samples were obtained. E. coli O157 serotype was isolated, and purified using sorbital MacConkey, MacConkey and nutrient agar. Serotyping was by card agglutination test. Oxoid verotoxin test kit was used to test for verotoxin (VT1 and VT2) production. Carrier environment was monitored. Knowledge, attitude and practices of meat transporters were assessed through a semi structured questionnaire and observations. Probability of contamination was modeled and run through Monte Carlo simulation using winBUGS®. Prevalence and data from the questionnaire were analysed using SPSS Ver.17. The contamination prevalence at offloading was significantly higher compared to loading (p = 0.05). The probability of obtaining an E. coli O157 serotype contaminated carcass at Dagoretti, Limuru and Eldoret, respectively, was 14, 16 and 19 at loading and 31, 39 and 66 at offloading per 1000 carcasses handled. The temperature in the meat carrier significantly increased (p = 0.004) during transportation between loading and offloading. The average time taken to transport the meat from the slaughterhouses to the butchery was 65 minutes. About 14 (43.8 %) of the meat transporters had worked in the meat industry for at least 5 years and almost an equal number 13 (40.4%) had had formal training on meat hygiene. About 53% of meat transporters claimed to wash hands regularly with cold water and soap. Meat carriers were cleaned at the river or in a car wash with cold water and soap only. Carcasses were loaded on the shoulders of the transportation personnel and placed on the floor of the carriers or heaped on top of other carcasses. Offloading at the butchery was done by the same person with no change over of clothes that could be soiled with blood. 2014-09-17 2014-10-07T09:11:21Z 2014-10-07T09:11:21Z Poster https://hdl.handle.net/10568/44923 en Open Access application/pdf University of Nairobi Kago, J., Kang’ethe, E., Wangoh, J. and Makita, K. 2014. Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya. Poster presented at the Tropentag 2014 Conference on Bridging the Gap between Increasing Knowledge and Decreasing Resources, Prague, Czech Republic, 17-19 September 2014. Nairobi, Kenya: University of Nairobi. |
| spellingShingle | health animal products zoonoses Kago, J. Kang'ethe, Erastus K. Wangoh, J. Makita, K. Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title | Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title_full | Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title_fullStr | Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title_full_unstemmed | Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title_short | Escherichia coli O157 serotype in beef carcasses post slaughterhouse in Nairobi and Eldoret, Kenya |
| title_sort | escherichia coli o157 serotype in beef carcasses post slaughterhouse in nairobi and eldoret kenya |
| topic | health animal products zoonoses |
| url | https://hdl.handle.net/10568/44923 |
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