Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz)
In the context set by the continuously growing agroindustrialization of cassava, and given that starch traits define tuber industrial potential, molecular tools that allow identifying variants of interest should be integrated into breeding programs for efficient selection of parental materials. The...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Español |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44400 |
| _version_ | 1855521502906023936 |
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| author | Tofiño Rivera, Adriana Patricia Cabal, D. Ceballos, H. Pérez, J.C. Romero, HM |
| author_browse | Cabal, D. Ceballos, H. Pérez, J.C. Romero, HM Tofiño Rivera, Adriana Patricia |
| author_facet | Tofiño Rivera, Adriana Patricia Cabal, D. Ceballos, H. Pérez, J.C. Romero, HM |
| author_sort | Tofiño Rivera, Adriana Patricia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In the context set by the continuously growing agroindustrialization of cassava, and given that starch traits define tuber industrial potential, molecular tools that allow identifying variants of interest should be integrated into breeding programs for efficient selection of parental materials. The present research study compared the TILLING reverse genetics technique (modified by directly visualizing cleaved products in agarose gel) to the traditional acrylamide gel and Li-Cor platform method. Both techniques were applied to polymorphism evaluation in 10 genes with major control of the starch metabolic route. A group of 150 M2 inbred lines derived from irradiated germplasm were phenotypically identified as probable mutants and analyzed by TILLING. Only 30% of the primers used produced good quality data, for most of them amplified more than one target. Additionally, visual detection of SNPs in agarose was not achieved in mixtures of DNA templates nor in individual samples, so the results cannot be said to correspond with those obtained by Li-Cor genotyping. |
| format | Journal Article |
| id | CGSpace44400 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| record_format | dspace |
| spelling | CGSpace444002025-05-28T14:52:33Z Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) Validation of TILLING in the evaluation of inbred progenies of irradiated cassava (Manihot esculenta Crantz) Tofiño Rivera, Adriana Patricia Cabal, D. Ceballos, H. Pérez, J.C. Romero, HM manihot esculenta genes polymorphism induced mutation starch quality polimorfismo mutación inducida almidón calidad In the context set by the continuously growing agroindustrialization of cassava, and given that starch traits define tuber industrial potential, molecular tools that allow identifying variants of interest should be integrated into breeding programs for efficient selection of parental materials. The present research study compared the TILLING reverse genetics technique (modified by directly visualizing cleaved products in agarose gel) to the traditional acrylamide gel and Li-Cor platform method. Both techniques were applied to polymorphism evaluation in 10 genes with major control of the starch metabolic route. A group of 150 M2 inbred lines derived from irradiated germplasm were phenotypically identified as probable mutants and analyzed by TILLING. Only 30% of the primers used produced good quality data, for most of them amplified more than one target. Additionally, visual detection of SNPs in agarose was not achieved in mixtures of DNA templates nor in individual samples, so the results cannot be said to correspond with those obtained by Li-Cor genotyping. En el marco de la creciente tendencia a la agroindustrialización de la yuca, y dado que las características del almidón definen el potencial industrial de estas raíces, aquellas herramientas moleculares que permitan identificar variantes de interés deben integrarse a programas de mejoramiento genético para seleccionar eficientemente nuevos materiales parentales. En este trabajo se compararon los resultados de la técnica TILLING de genética reversa (modificada en cuanto a la visualización directa de los productos de digestión en gel de agarosa), con el método tradicional que utiliza gel de acrilamida y plataforma Li-Cor. La comparación se llevó a cabo a través de la evaluación de polimorfismo en 10 genes con control metabólico mayor de la ruta del almidón. En 150 líneas endocriadas M2, derivadas de semillas irradiadas, fueron identificadas fenotípicamente como probables mutantes y analizadas por TILLING. Solo 30% de los cebadores utilizados produjo datos de buena calidad pues la mayoría amplificó más de un blanco. Adicionalmente, la detección visual de SNP en agarosa no corresponde con los registros obtenidos a partir del genotipado por Li-Cor, pues con ella no se logró detección de SNP, ni en plantillas de mezclas de ADN, ni en muestras individuales. 2009 2014-10-02T08:33:43Z 2014-10-02T08:33:43Z Journal Article https://hdl.handle.net/10568/44400 es Open Access |
| spellingShingle | manihot esculenta genes polymorphism induced mutation starch quality polimorfismo mutación inducida almidón calidad Tofiño Rivera, Adriana Patricia Cabal, D. Ceballos, H. Pérez, J.C. Romero, HM Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title | Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title_full | Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title_fullStr | Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title_full_unstemmed | Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title_short | Validación de TILLING en evaluación de progenies endocriadas de yuca irradiada (Manihot esculenta Crantz) |
| title_sort | validacion de tilling en evaluacion de progenies endocriadas de yuca irradiada manihot esculenta crantz |
| topic | manihot esculenta genes polymorphism induced mutation starch quality polimorfismo mutación inducida almidón calidad |
| url | https://hdl.handle.net/10568/44400 |
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