Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV)
Recent changes in the production systems of maize (Zea mays L.), including staggered and continue growing of the cultivation in Colombia, have increased the incidence of some diseases, among these stands out the sugar cane mosaic virus (SCMV). Several studies have demonstrated that the incidence of...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Español |
| Publicado: |
2001
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44388 |
| _version_ | 1855516285444554752 |
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| author | Salazar Erazo, Sandra Milena León, C. de |
| author_browse | León, C. de Salazar Erazo, Sandra Milena |
| author_facet | Salazar Erazo, Sandra Milena León, C. de |
| author_sort | Salazar Erazo, Sandra Milena |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Recent changes in the production systems of maize (Zea mays L.), including staggered and continue growing of the cultivation in Colombia, have increased the incidence of some diseases, among these stands out the sugar cane mosaic virus (SCMV). Several studies have demonstrated that the incidence of this disease can affect maize yields considerably. One of the most economic ways of solving the problem is using genetic resistance to the disease. The present research had as main objective to select resistant S1 lines to the SCMV from acid soil tolerant yellow and white maize base populations. In the cycle 1999A, 282, 155, 287 and 254 S1 lines were evaluated, derived from the acid soil tolerant maize populations SA3, SA4 (with yellow endosperm), SA6 and SA7 (with white endosperm). Seven-day old seedlings grown under screen house conditions, were mechanically was inoculated using leaf tissue from plants with characteristic symptoms of the virus. The infective tissue was ground in 10 - 15 ml phosphate buffer solution (0.1 M KPO4, pH 7.5) and inoculated rubbing in the leaves using carborundum as abrasive. The lines were evaluated 7 days after inoculation using the percentage of infected seedlings with symptoms of the disease. The results indicated that for the range of the 0 - 10 % infection, population SA7 showed the highest frequency of resistant lines to SCMV (76.4%), while populations SA4, SA3 and SA6 presented frequencies of 57.5, 49.5 and 27.5%, respectively. After evaluation 140, 89, 80, and 194 S1 lines were selected from population SA3, SA4, SA6 and SA7, respectively, as resistant to the virus. The obtained information is being utilized to increase the resistance levels to SCMV of the base maize populations and in the development of resistant synthetics and hybrids resistant to SCMV and tolerant to acid soil. |
| format | Journal Article |
| id | CGSpace44388 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| record_format | dspace |
| spelling | CGSpace443882025-11-12T05:53:29Z Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) Salazar Erazo, Sandra Milena León, C. de zea mays maize plant viruses genetic resistance plant breeding maíz virus de las plantas resistencia genética fitomejoramiento Recent changes in the production systems of maize (Zea mays L.), including staggered and continue growing of the cultivation in Colombia, have increased the incidence of some diseases, among these stands out the sugar cane mosaic virus (SCMV). Several studies have demonstrated that the incidence of this disease can affect maize yields considerably. One of the most economic ways of solving the problem is using genetic resistance to the disease. The present research had as main objective to select resistant S1 lines to the SCMV from acid soil tolerant yellow and white maize base populations. In the cycle 1999A, 282, 155, 287 and 254 S1 lines were evaluated, derived from the acid soil tolerant maize populations SA3, SA4 (with yellow endosperm), SA6 and SA7 (with white endosperm). Seven-day old seedlings grown under screen house conditions, were mechanically was inoculated using leaf tissue from plants with characteristic symptoms of the virus. The infective tissue was ground in 10 - 15 ml phosphate buffer solution (0.1 M KPO4, pH 7.5) and inoculated rubbing in the leaves using carborundum as abrasive. The lines were evaluated 7 days after inoculation using the percentage of infected seedlings with symptoms of the disease. The results indicated that for the range of the 0 - 10 % infection, population SA7 showed the highest frequency of resistant lines to SCMV (76.4%), while populations SA4, SA3 and SA6 presented frequencies of 57.5, 49.5 and 27.5%, respectively. After evaluation 140, 89, 80, and 194 S1 lines were selected from population SA3, SA4, SA6 and SA7, respectively, as resistant to the virus. The obtained information is being utilized to increase the resistance levels to SCMV of the base maize populations and in the development of resistant synthetics and hybrids resistant to SCMV and tolerant to acid soil. Los recientes cambios en los sistemas de producción de maíz (Zea mays L.), incluyendo siembras escalonadas y continúas del cultivo en Colombia, han incrementado la incidencia de algunas enfermedades, entre las que se destaca el mosaico de la caña de azúcar (SCMV). Estudios de varios investigadores han demostrado que la incidencia de ésta enfermedad puede afectar el rendimiento del maíz en forma considerable. Una de las formas más económicas de resolver el problema es usando materiales con resistencia genética a la enfermedad. El presente trabajo tuvo como objetivo seleccionar líneas S1 resistentes al SCMV de poblaciones amarillas y blancas de maíz tolerantes a suelos ácidos. En el ciclo 1999A se evaluaron 282, 155, 287 y 254 lineas S1 derivadas de las poblaciones base de maíz tolerantes a suelos ácidos SA3, SA4 (con endosperma amarillo), SA6 y SA7 (de endosperma blanco). Plántulas de 7 días de germinación en casa de malla, se inocularon mecánicamente utilizando tejido foliar de plantas con síntomas característicos del virus. El tejido enfermo se maceré en 10 -15 ml de solución amortiguadora (0,1 M KPO4, pH 7,5) y se inoculé frotando en las hojas utilizando carborundum como abrasivo. Las lineas se evaluaron 7 días después de inoculadas usando el porcentaje de plântulas infectadas con el virus. Los resultados obtenidos permitieron establecer que para el rango del 0 - 10 % de infección, la población SA7 presentó la más alta frecuencia de líneas resistentes al SCMV (76,4%), y las poblaciones SA4, SA3 y SA6 presentaron frecuencias de 57,5, 49,5 y 27,5 %, respectivamente. Después de inoculadas, se seleccionaron 140, 89, 80 y 194 lineas S1 de las poblaciones SA3, SA4, SA6 y SA7, respectivamente, como resistentes al virus. La información obtenida se utilizó para aumentar los niveles de resistencia al SCMV de las poblaciones base y en la formación de variedades sintéticas e híbridos resistentes al SCMV y tolerantes a suelos ácidos. 2001 2014-10-02T08:33:41Z 2014-10-02T08:33:41Z Journal Article https://hdl.handle.net/10568/44388 es Open Access application/pdf |
| spellingShingle | zea mays maize plant viruses genetic resistance plant breeding maíz virus de las plantas resistencia genética fitomejoramiento Salazar Erazo, Sandra Milena León, C. de Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title | Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title_full | Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title_fullStr | Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title_full_unstemmed | Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title_short | Selección de líneas de maíz (Zea mays L.) con resistencia al virus del mosaico de la caña de azúcar (SCMV) |
| title_sort | seleccion de lineas de maiz zea mays l con resistencia al virus del mosaico de la cana de azucar scmv |
| topic | zea mays maize plant viruses genetic resistance plant breeding maíz virus de las plantas resistencia genética fitomejoramiento |
| url | https://hdl.handle.net/10568/44388 |
| work_keys_str_mv | AT salazarerazosandramilena selecciondelineasdemaizzeamayslconresistenciaalvirusdelmosaicodelacanadeazucarscmv AT leoncde selecciondelineasdemaizzeamayslconresistenciaalvirusdelmosaicodelacanadeazucarscmv |