| Sumario: | Plants of SP 71-6163 variety positive for the virus of the sugarcane yellow leaf syndrome (ScYLV) were sowed under greenhouse conditions to purify the causal agent and to produce specific antibodies. The methodology used for purification was that of Lockhart (1999) modified by CENICAÑA, adding the Celluclast 1,5 L to 2% enzyme to the extraction buffer. The purified of the obtained virus were evaluated by electronic microscopy in the laboratory of the Virology Unit at CIAT inn order to verify viral particles concentration. Once obtained a high concentration of virus particles, the purified were put on a sucrose gradient with density of 10-40 % to increase the purity of the virus which was determined later in the spectrophotometer. The purified obtained was used as antigen in the production of inmunoglobulin by injecting New Zealand rabbits during four weeks by a concentration of 1mg ScYLV/ml. The antiserum was evaluated by ISEM and TBIA. High concentration of virus particles was found by adding 2 % Celluclast 1,5 L. The absorbance A200/A280 was of 1.49 and the approximate viral concentration of 1.87 mg/ml. The antibody produced will be used for serological tests of ISEM and TBIA in the diagnosis of the ScYLV in the sugarcane area of the Cauca Valley.
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