Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia
Entomopathogenic nematodes (EPN) of the groups Heterorhabditidae and Steinernematidae are used as potential control agents of soil pests, being found in many habitats worldwide according to studies of their distribution. We collected soil samples in four Colombian departments (Quindío, Risaralda, Ca...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Español |
| Publicado: |
2009
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| Acceso en línea: | https://hdl.handle.net/10568/44367 |
| _version_ | 1855538269612146688 |
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| author | Melo Molina, E.L. Ortega Ojeda, CA Susurluk, A Gaigl, A Bellotti, Anthony C. |
| author_browse | Bellotti, Anthony C. Gaigl, A Melo Molina, E.L. Ortega Ojeda, CA Susurluk, A |
| author_facet | Melo Molina, E.L. Ortega Ojeda, CA Susurluk, A Gaigl, A Bellotti, Anthony C. |
| author_sort | Melo Molina, E.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Entomopathogenic nematodes (EPN) of the groups Heterorhabditidae and Steinernematidae are used as potential control agents of soil pests, being found in many habitats worldwide according to studies of their distribution. We collected soil samples in four Colombian departments (Quindío, Risaralda, Caldas, and Cauca) in order to isolate and identify native EPN strains associated with whitegrub (Coleoptera: Melolonthidae) and burrower bug (Hemiptera: Cydnidae) Cyrtomenus bergi soil pests. EPN isolation was done through the Galleria mellonella (Lepidoptera: Pyralidae) bait method. After verifying the entomopathogenicity of the samples using Koch s postulates, we multiplied, stored and identified the EPN. We analyzed 284 samples (300 g of soil each) collected in 23 sites in 15 crops. Pathogenic and saprophytic nematodes were present in 17 samples. PCR technique lead to the identification of Steinernema kraussei Steiner (Rhabditida: Steinernematidae) coming from of two samples, one from Cassava (ex Manihot esculenta L.) in Cauca and the second one from Inga spp. in Risaralda, being these the first record of the species for the country. |
| format | Journal Article |
| id | CGSpace44367 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| record_format | dspace |
| spelling | CGSpace443672025-05-28T14:52:37Z Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia Native entomopathogenic nematodes (Rhabditida) in four departments of Colombia Melo Molina, E.L. Ortega Ojeda, CA Susurluk, A Gaigl, A Bellotti, Anthony C. manihot esculenta entomophilic nematodes nematodos entomófilos Entomopathogenic nematodes (EPN) of the groups Heterorhabditidae and Steinernematidae are used as potential control agents of soil pests, being found in many habitats worldwide according to studies of their distribution. We collected soil samples in four Colombian departments (Quindío, Risaralda, Caldas, and Cauca) in order to isolate and identify native EPN strains associated with whitegrub (Coleoptera: Melolonthidae) and burrower bug (Hemiptera: Cydnidae) Cyrtomenus bergi soil pests. EPN isolation was done through the Galleria mellonella (Lepidoptera: Pyralidae) bait method. After verifying the entomopathogenicity of the samples using Koch s postulates, we multiplied, stored and identified the EPN. We analyzed 284 samples (300 g of soil each) collected in 23 sites in 15 crops. Pathogenic and saprophytic nematodes were present in 17 samples. PCR technique lead to the identification of Steinernema kraussei Steiner (Rhabditida: Steinernematidae) coming from of two samples, one from Cassava (ex Manihot esculenta L.) in Cauca and the second one from Inga spp. in Risaralda, being these the first record of the species for the country. Nematodos entomopatógenos (NEP s) de los grupos Heterorhabditidae y Steinernematidae son usados como agentes potenciales de control de numerosas plagas subterráneas, encontrándoselos según estudios de su distribución, en diversidad de hábitats a través del mundo. En el mismo interés de control de rizófagos, especialmente las plagas del complejo chisas (Coleoptera: Melolonthidae) y chinche de la viruela Cyrtomenus bergi (Hemiptera: Cydnidae), se realizó una búsqueda e identificación de cepas nativas de NEP s, tomando muestras de suelos en Quindío, Risaralda, Caldas y Cauca. Para la extracción de NEP s se emplearon larvas trampa de Galleria mellonella (Lepidoptera: Pyralidae); después de comprobar su patogenicidad siguiendo los postulados de Koch, se multiplicaron, almacenaron e identificaron. De las 284 muestras (300 g de suelo c/u) de 15 cultivos, en 23 sitios 17 resultaron positivas para nematodos entomopatógenos y saprófagos. Usando la técnica PCR dos muestras fueron identificadas como Steinernema kraussei Steiner (Rhabditida: Steinernematidae), procedentes de yuca (ex Manihot esculenta L.) de Cauca y Risaralda (ex Inga spp.), constituyéndose en el primer reporte para Colombia. 2009 2014-10-02T08:33:39Z 2014-10-02T08:33:39Z Journal Article https://hdl.handle.net/10568/44367 es Open Access |
| spellingShingle | manihot esculenta entomophilic nematodes nematodos entomófilos Melo Molina, E.L. Ortega Ojeda, CA Susurluk, A Gaigl, A Bellotti, Anthony C. Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title | Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title_full | Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title_fullStr | Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title_full_unstemmed | Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title_short | Poblaciones nativas de nematodos entomopatógenos (Rhabditida) en cuatro departamentos de Colombia |
| title_sort | poblaciones nativas de nematodos entomopatogenos rhabditida en cuatro departamentos de colombia |
| topic | manihot esculenta entomophilic nematodes nematodos entomófilos |
| url | https://hdl.handle.net/10568/44367 |
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