Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia
Bacterial wilt or Moko of plantain, caused by Ralstonia solanacearum E.F. Smith, race 2, is the most important bacterial disease of this crop in Colombia. The BIO PCR technique was used for detecting the bacterium. A total of 337 samples were initially collected (193 from soil and 144 from plant tis...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Español |
| Published: |
2004
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| Online Access: | https://hdl.handle.net/10568/44341 |
| _version_ | 1855517200362766336 |
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| author | Gómez Calvo, Eduardo Augusto Álvarez, E. Llano Rodríguez, Germán Alberto |
| author_browse | Gómez Calvo, Eduardo Augusto Llano Rodríguez, Germán Alberto Álvarez, E. |
| author_facet | Gómez Calvo, Eduardo Augusto Álvarez, E. Llano Rodríguez, Germán Alberto |
| author_sort | Gómez Calvo, Eduardo Augusto |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Bacterial wilt or Moko of plantain, caused by Ralstonia solanacearum E.F. Smith, race 2, is the most important bacterial disease of this crop in Colombia. The BIO PCR technique was used for detecting the bacterium. A total of 337 samples were initially collected (193 from soil and 144 from plant tissue) from affected crops in six of Colombia s plantain-growing regions. DNA was extracted by thermolysis from 189 strains selected for their growth in SMSA medium; of these, 29 were isolated from soil and 160 from tissue. With primers OLI 1 and Y2, a 288-bp fragment could be amplified by PCR, from the 16S gene of ribosomal DNA in 98 tissue strains and 8 from soil. 64 of the tissue strains and the 8 from soil were selected according to origin and identified as biovar 1 by their production of acids from disaccharides cellobiose, lactose, and maltose, and oxidation of alcohols dulcitol, sorbitol, and manitol. Pathogenicity tests on seedlings of the plantain Africa (Musa cv. AAB) under greenhouse conditions showed that 35 strains were highly pathogenic, 28 moderately pathogenic, 8 had low pathogenicity, and 1 was nonpathogenic. For infiltration in tobacco leaves, 63 strains, induced hypersensitivity, and 9 induced yellowing, an atypical reaction for this race. BIO PCR technique facilitated the detection of the pathogen in soil and plants. |
| format | Journal Article |
| id | CGSpace44341 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| record_format | dspace |
| spelling | CGSpace443412025-11-12T05:53:40Z Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia Gómez Calvo, Eduardo Augusto Álvarez, E. Llano Rodríguez, Germán Alberto musa (plantains) ralstonia solanacearum identification pcr tropical fruits plant diseases musa (plátanos) identificación frutas tropicales enfermedades de las plantas Bacterial wilt or Moko of plantain, caused by Ralstonia solanacearum E.F. Smith, race 2, is the most important bacterial disease of this crop in Colombia. The BIO PCR technique was used for detecting the bacterium. A total of 337 samples were initially collected (193 from soil and 144 from plant tissue) from affected crops in six of Colombia s plantain-growing regions. DNA was extracted by thermolysis from 189 strains selected for their growth in SMSA medium; of these, 29 were isolated from soil and 160 from tissue. With primers OLI 1 and Y2, a 288-bp fragment could be amplified by PCR, from the 16S gene of ribosomal DNA in 98 tissue strains and 8 from soil. 64 of the tissue strains and the 8 from soil were selected according to origin and identified as biovar 1 by their production of acids from disaccharides cellobiose, lactose, and maltose, and oxidation of alcohols dulcitol, sorbitol, and manitol. Pathogenicity tests on seedlings of the plantain Africa (Musa cv. AAB) under greenhouse conditions showed that 35 strains were highly pathogenic, 28 moderately pathogenic, 8 had low pathogenicity, and 1 was nonpathogenic. For infiltration in tobacco leaves, 63 strains, induced hypersensitivity, and 9 induced yellowing, an atypical reaction for this race. BIO PCR technique facilitated the detection of the pathogen in soil and plants. La Marchitez bacteriana o Moko del plátano causado por Ralstonia solanacearum E.F. Smith raza 2 es la enfermedad bacteriana más importante de este cultivo en Colombia. La técnica de BIO-RCP, se utilizó para detectar la bacteria. Inicialmente se colectaron un total de 337 muestras (193 de suelo y 144 de tejido vegetal), provenientes de cultivos afectados en seis regiones productoras de plátano en Colombia. Se extrajo ADN mediante lisis por calor a 189 cepas seleccionadas por su crecimiento en SMSA, 29 de las cuales se aislaron de suelo y 160 de tejido. Mediante la técnica de la Reacción en Cadena de la Polimerasa (RCP), usando los cebadores OLI 1 y Y2, se amplificó un fragmento de 288 pb del gen 16S del ADN ribosomal de R. solanacearum en 98 cepas de tejido y 8 de suelo. 64 cepas de tejido y las 8 de suelo, se seleccionaron de acuerdo a su procedencia y se identificaron como biovar 1 con base en la producción de ácidos a partir de los disacáridos celobiosa, lactosa y maltosa y la oxidación de los alcoholes dulcitol, sorbitol y manitol. La prueba de patogenicidad en plántulas de plátano Africa (Musa cv. AAB) bajo condiciones de invernadero, mostró que 35 cepas fueron altamente patogénicas, 28 moderadamente patogénicas, 8 tuvieron patogenicidad baja y 1 no fue patogénica. Mediante infiltración en hojas de tabaco, 63 cepas indujeron reacción de hipersensibilidad correspondiente a la raza 2, y 9 cepas indujeron amarillamiento, reacción atípica para esta raza. La técnica de BIO RCP facilitó la detección del patógeno en suelo y plantas. 2004 2014-10-02T08:33:37Z 2014-10-02T08:33:37Z Journal Article https://hdl.handle.net/10568/44341 es Open Access application/pdf |
| spellingShingle | musa (plantains) ralstonia solanacearum identification pcr tropical fruits plant diseases musa (plátanos) identificación frutas tropicales enfermedades de las plantas Gómez Calvo, Eduardo Augusto Álvarez, E. Llano Rodríguez, Germán Alberto Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title | Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title_full | Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title_fullStr | Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title_full_unstemmed | Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title_short | Identificación y caracterización de cepas de Ralstonia solanacearum raza 2, agente causante del moko del plátano en Colombia |
| title_sort | identificacion y caracterizacion de cepas de ralstonia solanacearum raza 2 agente causante del moko del platano en colombia |
| topic | musa (plantains) ralstonia solanacearum identification pcr tropical fruits plant diseases musa (plátanos) identificación frutas tropicales enfermedades de las plantas |
| url | https://hdl.handle.net/10568/44341 |
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