| Sumario: | Endophytic fungi often develop a systemic and mutually beneficial association with their hosts. A wide range of economically important plants have been reported to harbor endophytes. In these symbiotic mutualisms, both host and symbiont gain benefits from the association. The fungus obtains nutrients form its host and in return it provides protection from abiotic (environmental stresses) and biotic stresses (pest and insect attacks) to its host plant. Endophytes have been shown to confer enhanced fitness to their hosts such as enhanced tillering, drought tolerance, root growth, overall enhanced plant growth. This work describes the detection, isolation and genetic transformation of an endophytic fungus, Acremonium implicatum, from Brachiaria brizantha accession CIAT 6780. The results open possibilities for exploiting the qualities of an introduced gene as a reporter and study the interactions between A. implicatum and its host Brachiaria. Furthermore, it also provides options to use a transformed A. implicatum as a vehicle for production and delivery of gene products of agronomic interest into the host plant in order to enhance protective benefits and other traits of agronomic importance that will contribute to improved plant productivity.
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