Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae)
Cyrtomenus bergi Froeschner is a subterranean phytophage important in tropical crops. We tested two commercial entomopathogenic nematode (epn) strains (Steinernema feltiae (Filipjev) and Heterorhabditis bacteriophora Poinar) as biological antagonists in the greenhouse (18-28°C and 55-85% relative hu...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Español |
| Publicado: |
2006
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44313 |
| _version_ | 1855528653817905152 |
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| author | Melo Molina, E.L. Ortega Ojeda, CA Gaigl, A Ehlers, R.U. Bellotti, Anthony C. |
| author_browse | Bellotti, Anthony C. Ehlers, R.U. Gaigl, A Melo Molina, E.L. Ortega Ojeda, CA |
| author_facet | Melo Molina, E.L. Ortega Ojeda, CA Gaigl, A Ehlers, R.U. Bellotti, Anthony C. |
| author_sort | Melo Molina, E.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cyrtomenus bergi Froeschner is a subterranean phytophage important in tropical crops. We tested two commercial entomopathogenic nematode (epn) strains (Steinernema feltiae (Filipjev) and Heterorhabditis bacteriophora Poinar) as biological antagonists in the greenhouse (18-28°C and 55-85% relative humidity). We used the concentrations recommended by the supplier E-nema: 1000 and 500 epn/ ml of S. feltiae and H. bacteriophora, respectively. These solutions were applied to pots of 1,5 dm3 filled with agricultural soil and sand (1:1) with 20 burrower bugs (adults or 5th instar immatures) confined to each pot. The insects were fed on groundnuts (Arachis hypogaea L.). Infestation rate of the burrower bug and mortality were measured through destructive evaluations 15 and 30 days after application (daa), three pots per time period. Only adults were infested (P£0,05). Considering both evaluations and all strains, we observed the greatest penetration rate for S. feltiae (93,9%) compared to H. bacteriophora (72,1%). In terms of mortality, H. bacteriophora killed more bugs after 15 days (42,2%) than S. feltiae (8,6%); however, after 30 days no differences were found between the two epn strains (54,3%). Moreover, we observed melanized nematodes, probably as an immune response of C. bergi against nematodes, where S. feltiae (37,5%) was more susceptible than H. bacteriophora (13,1%) at 30 daa. We conclude that only adults of C. bergi are susceptible to entomonematodes; H. bacteriophora is the most promising strain for producing greater mortality in less time and presenting a lower percent of melanization under these conditions. |
| format | Journal Article |
| id | CGSpace44313 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| record_format | dspace |
| spelling | CGSpace443132025-05-28T14:52:28Z Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) Evaluation of two commercial strains of entomonematodes as control agents of Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) Melo Molina, E.L. Ortega Ojeda, CA Gaigl, A Ehlers, R.U. Bellotti, Anthony C. cyrtomenus bergi biological control entomophilic nematodes control biológico nematodos entomófilos Cyrtomenus bergi Froeschner is a subterranean phytophage important in tropical crops. We tested two commercial entomopathogenic nematode (epn) strains (Steinernema feltiae (Filipjev) and Heterorhabditis bacteriophora Poinar) as biological antagonists in the greenhouse (18-28°C and 55-85% relative humidity). We used the concentrations recommended by the supplier E-nema: 1000 and 500 epn/ ml of S. feltiae and H. bacteriophora, respectively. These solutions were applied to pots of 1,5 dm3 filled with agricultural soil and sand (1:1) with 20 burrower bugs (adults or 5th instar immatures) confined to each pot. The insects were fed on groundnuts (Arachis hypogaea L.). Infestation rate of the burrower bug and mortality were measured through destructive evaluations 15 and 30 days after application (daa), three pots per time period. Only adults were infested (P£0,05). Considering both evaluations and all strains, we observed the greatest penetration rate for S. feltiae (93,9%) compared to H. bacteriophora (72,1%). In terms of mortality, H. bacteriophora killed more bugs after 15 days (42,2%) than S. feltiae (8,6%); however, after 30 days no differences were found between the two epn strains (54,3%). Moreover, we observed melanized nematodes, probably as an immune response of C. bergi against nematodes, where S. feltiae (37,5%) was more susceptible than H. bacteriophora (13,1%) at 30 daa. We conclude that only adults of C. bergi are susceptible to entomonematodes; H. bacteriophora is the most promising strain for producing greater mortality in less time and presenting a lower percent of melanization under these conditions. Cyrtomenus bergi Froeschner es un fitófago subterráneo importante en cultivos tropicales. Se evaluaron dos cepas comerciales de nemátodos entomopatógenos (nep) (Steinernema feltiae (Filipjev) y Heterorhabditis bacteriophora Poinar) como antagonistas biológicas bajo invernadero (18-28°C y 55-85% humedad relativa). Se utilizaron las concentraciones comerciales recomendadas por la casa commercial E-nema: 1000 y 500 nep/ml de S. feltiae y H. bacteriophora, respectivamente. Estas soluciones se aplicaron en potes de 1,5 dm3 con suelo agrícola y arena (1:1) con 20 individuos de C. bergi (adultos o inmaduros del quinto instar) confinados por pote. Los insectos fueron alimentados con plantas de maní (Arachis hypogaea L.). La tasa de infestación en el chinche y la mortalidad fueron medidas por medio de evaluaciones destructivas a los 15 y 30 días después de infectados (ddi), tres potes por época. Solo los adultos resultaron infectados (P£0,05). Considerando ambas evaluaciones y todas las cepas, se observó la mayor tasa de penetración en S. feltiae (93,9%) comparado con H. bacteriophora (72,1%). En términos de mortalidad, a 15 ddi H. bacteriophora (42,2%) mató a más insectos que S. feltiae (8,6%); sin embargo, a los 30 ddi no se encontraron diferencias entre las dos cepas de nep (ca. 54,3%). Adicionalmente, se observaron nemátodos melanizados, probablemente como respuesta inmunológica de C. bergi al entomopatógeno, donde S. feltiae (37,5%) fue más susceptible que H. bacteriophora (13,1%) a los 30 ddi. Se concluye que solo los chinches adultos son significativamente susceptibles a los entomonemátodos; H. bacteriophora es la cepa más promisoria al obtener las mayores mortalidades en menos tiempo y presentar el menor porcentaje de melanización bajo estas condiciones. 2006 2014-10-02T08:33:35Z 2014-10-02T08:33:35Z Journal Article https://hdl.handle.net/10568/44313 es Open Access |
| spellingShingle | cyrtomenus bergi biological control entomophilic nematodes control biológico nematodos entomófilos Melo Molina, E.L. Ortega Ojeda, CA Gaigl, A Ehlers, R.U. Bellotti, Anthony C. Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title | Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title_full | Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title_fullStr | Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title_full_unstemmed | Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title_short | Evaluación de dos cepas comerciales de entomonematodos como agentes de control de Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) |
| title_sort | evaluacion de dos cepas comerciales de entomonematodos como agentes de control de cyrtomenus bergi froeschner hemiptera cydnidae |
| topic | cyrtomenus bergi biological control entomophilic nematodes control biológico nematodos entomófilos |
| url | https://hdl.handle.net/10568/44313 |
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