Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos
Genetic variability of 116 accesions of Phaseolus vulgaris showing snap beans characteristics coming from primary and secondary centers of domestication, were studied using eighteen morphological descriptors to characterize pods and seeds, SDS-PAGE analysis of seed phaseolins and eight isozyme syste...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Español |
| Published: |
2004
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/44307 |
| _version_ | 1855513711533359104 |
|---|---|
| author | Tofiño Rivera, Adriana Patricia Gutiérrez, J.A. Ocampo Nahar, César H. García, V.H. |
| author_browse | García, V.H. Gutiérrez, J.A. Ocampo Nahar, César H. Tofiño Rivera, Adriana Patricia |
| author_facet | Tofiño Rivera, Adriana Patricia Gutiérrez, J.A. Ocampo Nahar, César H. García, V.H. |
| author_sort | Tofiño Rivera, Adriana Patricia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic variability of 116 accesions of Phaseolus vulgaris showing snap beans characteristics coming from primary and secondary centers of domestication, were studied using eighteen morphological descriptors to characterize pods and seeds, SDS-PAGE analysis of seed phaseolins and eight isozyme systems. Higher morphological diversity and best pod marketing characteristics were found at Andean accessions. After SDS-PAGE analysis Andean (T, C, and H) and Mesoamerican [S, Sb, CH and H(S+1)] types of phaseolin were detected in the sample, suggesting a higher contribution of the Mesoamerican gene pool to the genotype of snap beans. Also it was observed that, at the same zones of collection in some secondary centers, snap beans were originated and dispersed differently as compared to dry beans. A higher number of hybrid genotypes were detected among genetic pools when the three markers were used. Results seems to indicate that snap beans developed under environmental effects of the temperate zones, have a more complex genetic structure and higher rates of allogamy than those observed at primary centers of domestication. The results obtained using the three descriptors show that total genetic diversity for snap beans is similar to that of dry beans, but the snap beans population structure observed in this study is different to that reported for dry beans. Key words: Genetic variability, morphological descriptors, isozymes, seed protein, genetic pool. |
| format | Journal Article |
| id | CGSpace44307 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| record_format | dspace |
| spelling | CGSpace443072025-05-15T09:40:26Z Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos Tofiño Rivera, Adriana Patricia Gutiérrez, J.A. Ocampo Nahar, César H. García, V.H. phaseolus vulgaris genetic variation agronomic characters globulins variación genética características agronómicas globulinas Genetic variability of 116 accesions of Phaseolus vulgaris showing snap beans characteristics coming from primary and secondary centers of domestication, were studied using eighteen morphological descriptors to characterize pods and seeds, SDS-PAGE analysis of seed phaseolins and eight isozyme systems. Higher morphological diversity and best pod marketing characteristics were found at Andean accessions. After SDS-PAGE analysis Andean (T, C, and H) and Mesoamerican [S, Sb, CH and H(S+1)] types of phaseolin were detected in the sample, suggesting a higher contribution of the Mesoamerican gene pool to the genotype of snap beans. Also it was observed that, at the same zones of collection in some secondary centers, snap beans were originated and dispersed differently as compared to dry beans. A higher number of hybrid genotypes were detected among genetic pools when the three markers were used. Results seems to indicate that snap beans developed under environmental effects of the temperate zones, have a more complex genetic structure and higher rates of allogamy than those observed at primary centers of domestication. The results obtained using the three descriptors show that total genetic diversity for snap beans is similar to that of dry beans, but the snap beans population structure observed in this study is different to that reported for dry beans. Key words: Genetic variability, morphological descriptors, isozymes, seed protein, genetic pool. Se cuantificó la variabilidad genética de una muestra de 116 accesiones de habichuela P. vulgaris, cultivadas en centros primarios y secundarios de domesticación. Se evaluaron 18 descriptores morfo-agronómicos asociados con características de la planta, vaina y semilla. Mediante el análisis de las faseolinas utilizando SDS-PAGE se encontraron patrones de bandas de origen andino (T, C y H1) y mesoamericano [S, Sb, CH y H(S+I)]. También se evaluaron ocho sistemas isoenzimáticos polimórficos. En el germoplasma de habichuela hay importante contribución del acervo mesoamericano y las accesiones en algunos centros secundarios de domesticación tuvieron origen y procesos de dispersión diferentes de los del fríjol común en tales zonas. La mayor variabilidad morfológica y el mayor número de accesiones con características deseables para el mercado fresco se encontró en el grupo mesoamericano. Se detectó mayor número de genotipos híbridos entre acervos cuando se utilizaron simultáneamente los tres descriptores, lo cual indica una estructura genética compleja que podría deberse al efecto de los factores ambientales propios de la zona templada sobre sus patrones reproductivos. La diversidad total medida con los tres descriptores fue similar a la registrada en fríjol común. Sin embargo, la estructura poblacional encontrada por otros autores en el fríjol común es diferente de la observada en este estudio. Palabras claves: Variabilidad, descriptores morfológicos, isoenzimas, proteínas de semilla, acervos genéticos. 2004 2014-10-02T08:33:34Z 2014-10-02T08:33:34Z Journal Article https://hdl.handle.net/10568/44307 es Open Access Tofiño, A.P., J.A. Gutiérrez, C. Ocampo, V.H. García. 2004. Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos. Acta Agron. (Colombia) 53(1/2): 25-36. |
| spellingShingle | phaseolus vulgaris genetic variation agronomic characters globulins variación genética características agronómicas globulinas Tofiño Rivera, Adriana Patricia Gutiérrez, J.A. Ocampo Nahar, César H. García, V.H. Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title | Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title_full | Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title_fullStr | Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title_full_unstemmed | Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title_short | Estudio de la variabilidad genética en habichuela Phaseolus vulgaris L., mediante descriptores morfológicos y bioquímicos |
| title_sort | estudio de la variabilidad genetica en habichuela phaseolus vulgaris l mediante descriptores morfologicos y bioquimicos |
| topic | phaseolus vulgaris genetic variation agronomic characters globulins variación genética características agronómicas globulinas |
| url | https://hdl.handle.net/10568/44307 |
| work_keys_str_mv | AT tofinoriveraadrianapatricia estudiodelavariabilidadgeneticaenhabichuelaphaseolusvulgarislmediantedescriptoresmorfologicosybioquimicos AT gutierrezja estudiodelavariabilidadgeneticaenhabichuelaphaseolusvulgarislmediantedescriptoresmorfologicosybioquimicos AT ocamponaharcesarh estudiodelavariabilidadgeneticaenhabichuelaphaseolusvulgarislmediantedescriptoresmorfologicosybioquimicos AT garciavh estudiodelavariabilidadgeneticaenhabichuelaphaseolusvulgarislmediantedescriptoresmorfologicosybioquimicos |