Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections
The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme patt...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
1999
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44211 |
| _version_ | 1855526413733462016 |
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| author | Chavarriaga Aguirre, Paul Maya, M.M. Tohme, Joseph M. Duque E., Myriam Cristina Iglesias, C.A. Bonierbale, Merideth W. Kresovich, Stephen Kochert, G |
| author_browse | Bonierbale, Merideth W. Chavarriaga Aguirre, Paul Duque E., Myriam Cristina Iglesias, C.A. Kochert, G Kresovich, Stephen Maya, M.M. Tohme, Joseph M. |
| author_facet | Chavarriaga Aguirre, Paul Maya, M.M. Tohme, Joseph M. Duque E., Myriam Cristina Iglesias, C.A. Bonierbale, Merideth W. Kresovich, Stephen Kochert, G |
| author_sort | Chavarriaga Aguirre, Paul |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme patterns and specific agronomic criteria. To asses the genetic diversity of the core, 521 accessions were typed with four microsatellite loci. Allele diversity and frequency, and size variance of dinucleotide repeats (Rst statistic) were estimated. Microsatellite allele numbers and frequencies varied among countries: Colombia and Brazil had the largest number of different alleles across all loci. Mexico also had a high number, ranking fifth after Peru, Costa Rica and Venezuela (which tied). Unique alleles were present in accessions from Brazil, Colombia, Guatemala, Venezuela and Paraguay. A small number (1.34%) of potential duplicates were identified through isozyme and AFLP profiles. Thus, the present results indicated that traditional markers have been highly effective at selecting unique genotypes for the core. Future selections of cassava germplasm sets can be aided by DNA-based markers to ensure genetically representative, non-redundant samples. |
| format | Journal Article |
| id | CGSpace44211 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace442112024-08-29T11:41:31Z Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections Chavarriaga Aguirre, Paul Maya, M.M. Tohme, Joseph M. Duque E., Myriam Cristina Iglesias, C.A. Bonierbale, Merideth W. Kresovich, Stephen Kochert, G manihot esculenta gene banks genetic markers germplasm genetic variation microsatellites aflp banco de genes marcadores genéticos germoplasma variación genética microsatélites The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme patterns and specific agronomic criteria. To asses the genetic diversity of the core, 521 accessions were typed with four microsatellite loci. Allele diversity and frequency, and size variance of dinucleotide repeats (Rst statistic) were estimated. Microsatellite allele numbers and frequencies varied among countries: Colombia and Brazil had the largest number of different alleles across all loci. Mexico also had a high number, ranking fifth after Peru, Costa Rica and Venezuela (which tied). Unique alleles were present in accessions from Brazil, Colombia, Guatemala, Venezuela and Paraguay. A small number (1.34%) of potential duplicates were identified through isozyme and AFLP profiles. Thus, the present results indicated that traditional markers have been highly effective at selecting unique genotypes for the core. Future selections of cassava germplasm sets can be aided by DNA-based markers to ensure genetically representative, non-redundant samples. 1999 2014-10-02T08:33:26Z 2014-10-02T08:33:26Z Journal Article https://hdl.handle.net/10568/44211 en Limited Access Springer |
| spellingShingle | manihot esculenta gene banks genetic markers germplasm genetic variation microsatellites aflp banco de genes marcadores genéticos germoplasma variación genética microsatélites Chavarriaga Aguirre, Paul Maya, M.M. Tohme, Joseph M. Duque E., Myriam Cristina Iglesias, C.A. Bonierbale, Merideth W. Kresovich, Stephen Kochert, G Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title | Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title_full | Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title_fullStr | Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title_full_unstemmed | Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title_short | Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections |
| title_sort | using microsatellites isozymes and aflps to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of dna based markers to maintain germplasm collections |
| topic | manihot esculenta gene banks genetic markers germplasm genetic variation microsatellites aflp banco de genes marcadores genéticos germoplasma variación genética microsatélites |
| url | https://hdl.handle.net/10568/44211 |
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