Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation
Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledons w...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2000
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44199 |
| _version_ | 1855530839034560512 |
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| author | Sarria, Rodrigo A. Torres Castro, Edgar Alonso Angel, F. Chavarriaga Aguirre, Paul Roca, W. |
| author_browse | Angel, F. Chavarriaga Aguirre, Paul Roca, W. Sarria, Rodrigo A. Torres Castro, Edgar Alonso |
| author_facet | Sarria, Rodrigo A. Torres Castro, Edgar Alonso Angel, F. Chavarriaga Aguirre, Paul Roca, W. |
| author_sort | Sarria, Rodrigo A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants. |
| format | Journal Article |
| id | CGSpace44199 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace441992024-08-27T10:36:26Z Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation Sarria, Rodrigo A. Torres Castro, Edgar Alonso Angel, F. Chavarriaga Aguirre, Paul Roca, W. manihot esculenta transgenic plants agrobacterium resistencia to chemicals plantas transgénicas resistencia a productos químicos Transgenic plants of cassava (Manihot esculenta) resistant to the herbicide Basta were obtained through Agrobacterium-mediated transformation. The plants also expressed the uidA gene and two were positive for PCR- and/or Southern-based detection of the nptII gene. Somatic-embryo-derived cotyledons were used as source of explants. A non-disarmed Agrobacterium strain (CIAT 1182) was used to transfer the genes of interest into cassava cultivar MPer183. Greenhouse tests of resistance to Basta (Hoechst) showed three plant lines with different levels of tolerance to the herbicide. Based on Southern tests of transgenesis, the transformation efficiency was 1%. The results constitute the first report of the bar gene conferring herbicide resistance to cassava plants. 2000-03-13 2014-10-02T08:33:24Z 2014-10-02T08:33:24Z Journal Article https://hdl.handle.net/10568/44199 en Limited Access Springer |
| spellingShingle | manihot esculenta transgenic plants agrobacterium resistencia to chemicals plantas transgénicas resistencia a productos químicos Sarria, Rodrigo A. Torres Castro, Edgar Alonso Angel, F. Chavarriaga Aguirre, Paul Roca, W. Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title | Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title_full | Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title_fullStr | Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title_full_unstemmed | Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title_short | Transgenic plants of casssva (Manihot esculenta) with resistance to basta obtained by Agrobacterium-mediated transformation |
| title_sort | transgenic plants of casssva manihot esculenta with resistance to basta obtained by agrobacterium mediated transformation |
| topic | manihot esculenta transgenic plants agrobacterium resistencia to chemicals plantas transgénicas resistencia a productos químicos |
| url | https://hdl.handle.net/10568/44199 |
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