Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers

Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguicula...

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Autores principales: Ocampo Nahar, César H., Martin, J,P, Sánchez Yélamo, María Dolores, Ortíz, J.M., Toro Chica, Orlando
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2005
Materias:
Acceso en línea:https://hdl.handle.net/10568/44194
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author Ocampo Nahar, César H.
Martin, J,P,
Sánchez Yélamo, María Dolores
Ortíz, J.M.
Toro Chica, Orlando
author_browse Martin, J,P,
Ocampo Nahar, César H.
Ortíz, J.M.
Sánchez Yélamo, María Dolores
Toro Chica, Orlando
author_facet Ocampo Nahar, César H.
Martin, J,P,
Sánchez Yélamo, María Dolores
Ortíz, J.M.
Toro Chica, Orlando
author_sort Ocampo Nahar, César H.
collection Repository of Agricultural Research Outputs (CGSpace)
description Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguiculata) were also included. RAPD analysis of DNA leaf extracts were carried out with four selected primers. We also studied morphological characters of the seeds and the phaseolin electrophoretic patterns. Multivariate analysis with the UPGMA method using RAPD data clustered the samples in four groups and, comparing with morphological data and phaseolin types, showed that the Spanish cultivars were mainly of Andean origin. Nevertheless, occurrence of introgression in Spain and the consideration of the country as a second center of variability for beans can also explain the obtained results.
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spelling CGSpace441942024-08-27T10:35:46Z Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers Ocampo Nahar, César H. Martin, J,P, Sánchez Yélamo, María Dolores Ortíz, J.M. Toro Chica, Orlando phaseolus vulgaris phaseolus coccineus vigna unguiculata varieties wild plants provenance rapd dna genetic variation genetic markers globulins spain variedades plantas silvestres procedencia adn variación genética marcadores genéticos globulinas españa Fifty-four accessions of Phaseolus vulgaris cultivated in Spain and representing a broad variability for this country, were studied together with 30 samples of wild forms of American origin. Two reference cultivars (from the Andes and Mesoamerica) plus two outgroups (P. coccineus and Vigna unguiculata) were also included. RAPD analysis of DNA leaf extracts were carried out with four selected primers. We also studied morphological characters of the seeds and the phaseolin electrophoretic patterns. Multivariate analysis with the UPGMA method using RAPD data clustered the samples in four groups and, comparing with morphological data and phaseolin types, showed that the Spanish cultivars were mainly of Andean origin. Nevertheless, occurrence of introgression in Spain and the consideration of the country as a second center of variability for beans can also explain the obtained results. 2005-02 2014-10-02T08:33:24Z 2014-10-02T08:33:24Z Journal Article https://hdl.handle.net/10568/44194 en Limited Access Springer Ocampo, César Humberto; Martín, Juan Pedro; Sánchez Yélamo, María Dolores; Ortiz, Jesús María; Toro Chica, Orlando. 2005. Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers. Genetic Resources and Crop Evolution (Netherlands). 52(1):33-40.
spellingShingle phaseolus vulgaris
phaseolus coccineus
vigna unguiculata
varieties
wild plants
provenance
rapd
dna
genetic variation
genetic markers
globulins
spain
variedades
plantas silvestres
procedencia
adn
variación genética
marcadores genéticos
globulinas
españa
Ocampo Nahar, César H.
Martin, J,P,
Sánchez Yélamo, María Dolores
Ortíz, J.M.
Toro Chica, Orlando
Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title_full Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title_fullStr Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title_full_unstemmed Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title_short Tracing the origin of Spanish common bean cultivars using biochemical and molecular markers
title_sort tracing the origin of spanish common bean cultivars using biochemical and molecular markers
topic phaseolus vulgaris
phaseolus coccineus
vigna unguiculata
varieties
wild plants
provenance
rapd
dna
genetic variation
genetic markers
globulins
spain
variedades
plantas silvestres
procedencia
adn
variación genética
marcadores genéticos
globulinas
españa
url https://hdl.handle.net/10568/44194
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