The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification
The Tpv2 family of transposable elements of common bean, Phaseolus vulgaris, belongs to the Ty1/copia group of long terminal repeat (LTR) containing retrotransposons. By reverse transcriptase (RT)-PCR and by analysis of genomic clones, we characterized four of the approximately 40 copies present in...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
1999
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/44182 |
| _version_ | 1855520997891899392 |
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| author | Garber, K Bilic, I Pusch, O Tohme, Joseph M. Bachmair, A Schweizer, D. Jantsch, V |
| author_browse | Bachmair, A Bilic, I Garber, K Jantsch, V Pusch, O Schweizer, D. Tohme, Joseph M. |
| author_facet | Garber, K Bilic, I Pusch, O Tohme, Joseph M. Bachmair, A Schweizer, D. Jantsch, V |
| author_sort | Garber, K |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The Tpv2 family of transposable elements of common bean, Phaseolus vulgaris, belongs to the Ty1/copia group of long terminal repeat (LTR) containing retrotransposons. By reverse transcriptase (RT)-PCR and by analysis of genomic clones, we characterized four of the approximately 40 copies present in the Phaseolus genome, and the genomic environment of their integration sites. Tpv2 integrated preferentially into actively transcribed regions. While none of the isolated elements had all the functional domains necessary for transposition, analysis of bean cultivars suggested that some members of the Tpv2 family transposed in recent breeding history. Probes derived from Tpv2, as well as flanking genomic sequences, may be useful for classifying Phaseolus cultivars. |
| format | Journal Article |
| id | CGSpace44182 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace441822024-08-29T11:41:29Z The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification Garber, K Bilic, I Pusch, O Tohme, Joseph M. Bachmair, A Schweizer, D. Jantsch, V phaseolus vulgaris nucleotide sequence gene transposition genotypes varieties gene expression messenger rna ultraviolet radiation salicylic acid pseudomonas syringae experimental infection secuencia nucleica transposición de genes genotipos variedades expresión génica arn mensajero irradiación ultravioleta acido salicílico infección experimental The Tpv2 family of transposable elements of common bean, Phaseolus vulgaris, belongs to the Ty1/copia group of long terminal repeat (LTR) containing retrotransposons. By reverse transcriptase (RT)-PCR and by analysis of genomic clones, we characterized four of the approximately 40 copies present in the Phaseolus genome, and the genomic environment of their integration sites. Tpv2 integrated preferentially into actively transcribed regions. While none of the isolated elements had all the functional domains necessary for transposition, analysis of bean cultivars suggested that some members of the Tpv2 family transposed in recent breeding history. Probes derived from Tpv2, as well as flanking genomic sequences, may be useful for classifying Phaseolus cultivars. 1999 2014-10-02T08:33:23Z 2014-10-02T08:33:23Z Journal Article https://hdl.handle.net/10568/44182 en Limited Access Springer |
| spellingShingle | phaseolus vulgaris nucleotide sequence gene transposition genotypes varieties gene expression messenger rna ultraviolet radiation salicylic acid pseudomonas syringae experimental infection secuencia nucleica transposición de genes genotipos variedades expresión génica arn mensajero irradiación ultravioleta acido salicílico infección experimental Garber, K Bilic, I Pusch, O Tohme, Joseph M. Bachmair, A Schweizer, D. Jantsch, V The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title | The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title_full | The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title_fullStr | The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title_full_unstemmed | The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title_short | The Tpv2 family of retrotransposons of Phaseolus vulgaris: Structure, integration characteristics, and use of genotype classification |
| title_sort | tpv2 family of retrotransposons of phaseolus vulgaris structure integration characteristics and use of genotype classification |
| topic | phaseolus vulgaris nucleotide sequence gene transposition genotypes varieties gene expression messenger rna ultraviolet radiation salicylic acid pseudomonas syringae experimental infection secuencia nucleica transposición de genes genotipos variedades expresión génica arn mensajero irradiación ultravioleta acido salicílico infección experimental |
| url | https://hdl.handle.net/10568/44182 |
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