The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4
The complete nucleotide sequence of the genomic RNA of the NL4 strain of Bean common mosaic virus (BCMV-NL4) was determined. The viral genome is 10037 nucleotides in length, excluding the 3 terminal poly (A) tail, and contains a single open reading frame (ORF) of 9666 nucleotides encoding a polypro...
| Autores principales: | , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2008
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/44156 |
| _version_ | 1855543094202597376 |
|---|---|
| author | Bravo, E Calvert, Lee A. Morales, Francisco José |
| author_browse | Bravo, E Calvert, Lee A. Morales, Francisco José |
| author_facet | Bravo, E Calvert, Lee A. Morales, Francisco José |
| author_sort | Bravo, E |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The complete nucleotide sequence of the genomic RNA of the NL4 strain of Bean common mosaic virus (BCMV-NL4) was determined. The viral genome is 10037 nucleotides in length, excluding the 3 terminal poly (A) tail, and contains a single open reading frame (ORF) of 9666 nucleotides encoding a polyprotein of 3222 amino acids. . The ORF is flanked by 5 and 3 untranslated regions (UTRs) of 133 and 235 nucleotides, respectively. Comparative analyses of the predicted BCMV-NL4 polyprotein with other species of the genus Potyvirus revealed nine cleavage sites resulting in ten functional proteins. Nucleotide and amino acid sequence identities indicated a close relationship between BCMV- NL4 and BCMV-NL1. Blast comparisons using coat protein (CP) and 3 UTR sequences showed 100% identity between BCMV-NL4 and the Mexican variant (US6) of this strain. |
| format | Journal Article |
| id | CGSpace44156 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| record_format | dspace |
| spelling | CGSpace441562025-05-15T09:40:31Z The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 Bravo, E Calvert, Lee A. Morales, Francisco José phaseolus vulgaris potyviruses genomics bean common mosaic virus potyvirus genómica virus del mosaico común frijol The complete nucleotide sequence of the genomic RNA of the NL4 strain of Bean common mosaic virus (BCMV-NL4) was determined. The viral genome is 10037 nucleotides in length, excluding the 3 terminal poly (A) tail, and contains a single open reading frame (ORF) of 9666 nucleotides encoding a polyprotein of 3222 amino acids. . The ORF is flanked by 5 and 3 untranslated regions (UTRs) of 133 and 235 nucleotides, respectively. Comparative analyses of the predicted BCMV-NL4 polyprotein with other species of the genus Potyvirus revealed nine cleavage sites resulting in ten functional proteins. Nucleotide and amino acid sequence identities indicated a close relationship between BCMV- NL4 and BCMV-NL1. Blast comparisons using coat protein (CP) and 3 UTR sequences showed 100% identity between BCMV-NL4 and the Mexican variant (US6) of this strain. Se determinó la secuencia completa del ARN genómico del Virus del mosaico común del fríjol (Bean common mosaic virus) cepa NL4 (BCMV-NL4). El genoma viral consta de 10037 nucleótidos excluyendo la cola de poli (A) y contiene una trama de lectura abierta (ORF) de 9666 nucleótidos que codifica una poliproteína de 3222 aminoácidos. La ORF está flanqueada por dos regiones no traducidas (UTR): la 5 UTR de 133 nucleótidos y la 3 UTR de 235 nucleótidos. Análisis comparativos de la poliproteína del BCMV-NL4 con otras especies del género Potyvirus, reveló nueve sitios de clivaje que resultaron en las diez proteínas funcionales características de estos virus. Alineamientos múltiples de las secuencias de nucleótidos del ARN genómico y de aminoácidos de la poliproteína del BCMV-NL4 con las de otros miembros de la familia Potyviridae mostraron una relación estrecha entre el BCMV-NL4 y el BCMV-NL1. Las comparaciones mediante el uso del programa Blast de las secuencias de la proteína de la cápsida (CP) y de la 3 UTR, mostraron una identidad del 100% entre el BCMV-NL4 y la variante mexicana (US6) de esta cepa.. 2008 2014-10-02T08:33:21Z 2014-10-02T08:33:21Z Journal Article https://hdl.handle.net/10568/44156 en Open Access |
| spellingShingle | phaseolus vulgaris potyviruses genomics bean common mosaic virus potyvirus genómica virus del mosaico común frijol Bravo, E Calvert, Lee A. Morales, Francisco José The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title | The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title_full | The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title_fullStr | The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title_full_unstemmed | The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title_short | The complete nucleotide sequence of the genomic RNA of bean common mosaic virus strain NL4 |
| title_sort | complete nucleotide sequence of the genomic rna of bean common mosaic virus strain nl4 |
| topic | phaseolus vulgaris potyviruses genomics bean common mosaic virus potyvirus genómica virus del mosaico común frijol |
| url | https://hdl.handle.net/10568/44156 |
| work_keys_str_mv | AT bravoe thecompletenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 AT calvertleea thecompletenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 AT moralesfranciscojose thecompletenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 AT bravoe completenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 AT calvertleea completenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 AT moralesfranciscojose completenucleotidesequenceofthegenomicrnaofbeancommonmosaicvirusstrainnl4 |