QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population

Iron and zinc deficiencies are human health problems found throughout the world and biofortification is a plant breeding-based strategy to improve the staple crops that could address these dietary constraints. Common bean is an important legume crop with two major genepools that has been the focus o...

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Main Authors: Blair, Matthew W., Medina, JI, Astudillo, Carolina, Rengifo Medina, J, Beebe, Stephen E., Machado, G, Graham, RD
Format: Journal Article
Language:Inglés
Published: Springer 2010
Subjects:
Online Access:https://hdl.handle.net/10568/43990
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author Blair, Matthew W.
Medina, JI
Astudillo, Carolina
Rengifo Medina, J
Beebe, Stephen E.
Machado, G
Graham, RD
author_browse Astudillo, Carolina
Beebe, Stephen E.
Blair, Matthew W.
Graham, RD
Machado, G
Medina, JI
Rengifo Medina, J
author_facet Blair, Matthew W.
Medina, JI
Astudillo, Carolina
Rengifo Medina, J
Beebe, Stephen E.
Machado, G
Graham, RD
author_sort Blair, Matthew W.
collection Repository of Agricultural Research Outputs (CGSpace)
description Iron and zinc deficiencies are human health problems found throughout the world and biofortification is a plant breeding-based strategy to improve the staple crops that could address these dietary constraints. Common bean is an important legume crop with two major genepools that has been the focus of genetic improvement for seed micronutrient levels. The objective of this study was to evaluate the inheritance of seed iron and zinc concentrations and contents in an intra-genepool Mesoamerican × Mesoamerican recombinant inbred line population grown over three sites in Colombia and to identify quantitative trait loci (QTL) for each mineral. The population had 110 lines and was derived from a high-seed iron and zinc climbing bean genotype (G14519) crossed with a low-mineral Carioca-type, prostrate bush bean genotype (G4825). The genetic map for QTL analysis was created from SSR and RAPD markers covering all 11 chromosomes of the common bean genome. A set of across-site, overlapping iron and zinc QTL was discovered on linkage group b06 suggesting a possibly pleiotropic locus and common physiology for mineral uptake or loading. Other QTL for mineral concentration or content were found on linkage groups b02, b03, b04, b07, b08 and b11 and together with the b06 cluster were mostly novel compared to loci found in previous studies of the Andean genepool or inter-genepool crosses. The discovery of an important new locus for seed iron and zinc concentrations may facilitate crop improvement and biofortification using the high-mineral genotype especially within the Mesoamerican genepool.
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spelling CGSpace439902024-05-01T08:19:33Z QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population Blair, Matthew W. Medina, JI Astudillo, Carolina Rengifo Medina, J Beebe, Stephen E. Machado, G Graham, RD phaseolus vulgaris genotypes quantitative trait loci iron zinc genotipos loci de rasgos cuantitativos hierro cinc Iron and zinc deficiencies are human health problems found throughout the world and biofortification is a plant breeding-based strategy to improve the staple crops that could address these dietary constraints. Common bean is an important legume crop with two major genepools that has been the focus of genetic improvement for seed micronutrient levels. The objective of this study was to evaluate the inheritance of seed iron and zinc concentrations and contents in an intra-genepool Mesoamerican × Mesoamerican recombinant inbred line population grown over three sites in Colombia and to identify quantitative trait loci (QTL) for each mineral. The population had 110 lines and was derived from a high-seed iron and zinc climbing bean genotype (G14519) crossed with a low-mineral Carioca-type, prostrate bush bean genotype (G4825). The genetic map for QTL analysis was created from SSR and RAPD markers covering all 11 chromosomes of the common bean genome. A set of across-site, overlapping iron and zinc QTL was discovered on linkage group b06 suggesting a possibly pleiotropic locus and common physiology for mineral uptake or loading. Other QTL for mineral concentration or content were found on linkage groups b02, b03, b04, b07, b08 and b11 and together with the b06 cluster were mostly novel compared to loci found in previous studies of the Andean genepool or inter-genepool crosses. The discovery of an important new locus for seed iron and zinc concentrations may facilitate crop improvement and biofortification using the high-mineral genotype especially within the Mesoamerican genepool. 2010-10 2014-10-02T08:33:04Z 2014-10-02T08:33:04Z Journal Article https://hdl.handle.net/10568/43990 en Limited Access Springer Blair, Matthew W.; Medina, Juliana I.; Astudillo R., Carolina; Rengifo Medina, Judith; Beebe, Stephen E.; Machado, Gloria; Graham, Robin D.. 2010. QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population . Theoretical and Applied Genetics (Germany) 121:1059–1070.
spellingShingle phaseolus vulgaris
genotypes
quantitative trait loci
iron
zinc
genotipos
loci de rasgos cuantitativos
hierro
cinc
Blair, Matthew W.
Medina, JI
Astudillo, Carolina
Rengifo Medina, J
Beebe, Stephen E.
Machado, G
Graham, RD
QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title_full QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title_fullStr QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title_full_unstemmed QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title_short QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population
title_sort qtl for seed iron and zinc concentration and content in a mesoamerican common bean phaseolus vulgaris l population
topic phaseolus vulgaris
genotypes
quantitative trait loci
iron
zinc
genotipos
loci de rasgos cuantitativos
hierro
cinc
url https://hdl.handle.net/10568/43990
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