QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava

We evaluated cassava bacterial blight (CBB) infection in an pair-cross population of 150 individuals derived from an intra-specific cross between two non-inbred cassava (Manihot esculenta Crantz) lines. The replicated trials were carried out in the field under high disease pressure over two consecut...

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Autores principales: Jorge, V., Fregene, Martin A., Velez, C.M., Duque E., Myriam Cristina, Tohme, Joseph M., Verdier, Valerie M.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2001
Materias:
Acceso en línea:https://hdl.handle.net/10568/43988
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author Jorge, V.
Fregene, Martin A.
Velez, C.M.
Duque E., Myriam Cristina
Tohme, Joseph M.
Verdier, Valerie M.
author_browse Duque E., Myriam Cristina
Fregene, Martin A.
Jorge, V.
Tohme, Joseph M.
Velez, C.M.
Verdier, Valerie M.
author_facet Jorge, V.
Fregene, Martin A.
Velez, C.M.
Duque E., Myriam Cristina
Tohme, Joseph M.
Verdier, Valerie M.
author_sort Jorge, V.
collection Repository of Agricultural Research Outputs (CGSpace)
description We evaluated cassava bacterial blight (CBB) infection in an pair-cross population of 150 individuals derived from an intra-specific cross between two non-inbred cassava (Manihot esculenta Crantz) lines. The replicated trials were carried out in the field under high disease pressure over two consecutive crop cycles. Evaluations were conducted at 4 and 7 months after planting for the two cycles. Simple regression analysis and the nonparametric Kruskal-Wallis rank-sum test revealed that eight quantitative trait loci (QTLs) were involved in resistance. We detected changes in QTLs from crop cycle to crop cycle. The pathogen population (Xanthomonas axonopodis pv. manihotis) was also monitored over the period, using a restriction fragment length polymorphism probe and pathogenic tests. Changes in QTL detection over the 2 years could be correlated with changes in pathogen population structure. One QTL, located in linkage group D, was conserved over the two crop cycles, and in field to greenhouse evaluations. This study thus identified molecular markers useful for marker assisted-selection, a technique that can accelerate the long, multiple-season process of breeding for CBB resistance.
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spelling CGSpace439882024-08-27T10:36:35Z QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava Jorge, V. Fregene, Martin A. Velez, C.M. Duque E., Myriam Cristina Tohme, Joseph M. Verdier, Valerie M. manihot esculenta xanthomonas disease resistance genetic markers field resistance quantitative trait loci bacterial blight marker-assisted selection resistencia a la enfermedad marcadores genéticos We evaluated cassava bacterial blight (CBB) infection in an pair-cross population of 150 individuals derived from an intra-specific cross between two non-inbred cassava (Manihot esculenta Crantz) lines. The replicated trials were carried out in the field under high disease pressure over two consecutive crop cycles. Evaluations were conducted at 4 and 7 months after planting for the two cycles. Simple regression analysis and the nonparametric Kruskal-Wallis rank-sum test revealed that eight quantitative trait loci (QTLs) were involved in resistance. We detected changes in QTLs from crop cycle to crop cycle. The pathogen population (Xanthomonas axonopodis pv. manihotis) was also monitored over the period, using a restriction fragment length polymorphism probe and pathogenic tests. Changes in QTL detection over the 2 years could be correlated with changes in pathogen population structure. One QTL, located in linkage group D, was conserved over the two crop cycles, and in field to greenhouse evaluations. This study thus identified molecular markers useful for marker assisted-selection, a technique that can accelerate the long, multiple-season process of breeding for CBB resistance. 2001-03 2014-10-02T08:33:03Z 2014-10-02T08:33:03Z Journal Article https://hdl.handle.net/10568/43988 en Limited Access Springer
spellingShingle manihot esculenta
xanthomonas
disease resistance
genetic markers
field resistance
quantitative trait loci
bacterial blight
marker-assisted selection
resistencia a la enfermedad
marcadores genéticos
Jorge, V.
Fregene, Martin A.
Velez, C.M.
Duque E., Myriam Cristina
Tohme, Joseph M.
Verdier, Valerie M.
QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title_full QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title_fullStr QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title_full_unstemmed QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title_short QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
title_sort qtl analysis of field resistance to xanthomonas axonopodis pv manihotis in cassava
topic manihot esculenta
xanthomonas
disease resistance
genetic markers
field resistance
quantitative trait loci
bacterial blight
marker-assisted selection
resistencia a la enfermedad
marcadores genéticos
url https://hdl.handle.net/10568/43988
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