Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]
Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumula...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2010
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43980 |
| _version_ | 1855514428709011456 |
|---|---|
| author | Welsch, R Arango, Jacobo Bar, C Salazar, B. Al-Babili, S. Beltrán Giraldo, Jorge Alonso Chavarriaga Aguirre, Paul Ceballos, H. Tohme, Joseph M. Beyer, Peter |
| author_browse | Al-Babili, S. Arango, Jacobo Bar, C Beltrán Giraldo, Jorge Alonso Beyer, Peter Ceballos, H. Chavarriaga Aguirre, Paul Salazar, B. Tohme, Joseph M. Welsch, R |
| author_facet | Welsch, R Arango, Jacobo Bar, C Salazar, B. Al-Babili, S. Beltrán Giraldo, Jorge Alonso Chavarriaga Aguirre, Paul Ceballos, H. Tohme, Joseph M. Beyer, Peter |
| author_sort | Welsch, R |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored provitamin A carotenoids in storage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants overexpressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterized PSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification. |
| format | Journal Article |
| id | CGSpace43980 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace439802024-08-27T10:34:47Z Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] Welsch, R Arango, Jacobo Bar, C Salazar, B. Al-Babili, S. Beltrán Giraldo, Jorge Alonso Chavarriaga Aguirre, Paul Ceballos, H. Tohme, Joseph M. Beyer, Peter manihot esculenta varieties vitamins carotenoids plant breeding polymorphism retinol roots variedades vitaminas carotenoides mejoramiento de plantas polimorfismo raíces cell biology Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored provitamin A carotenoids in storage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants overexpressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterized PSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification. 2010-11-24 2014-10-02T08:33:03Z 2014-10-02T08:33:03Z Journal Article https://hdl.handle.net/10568/43980 en Open Access Oxford University Press |
| spellingShingle | manihot esculenta varieties vitamins carotenoids plant breeding polymorphism retinol roots variedades vitaminas carotenoides mejoramiento de plantas polimorfismo raíces cell biology Welsch, R Arango, Jacobo Bar, C Salazar, B. Al-Babili, S. Beltrán Giraldo, Jorge Alonso Chavarriaga Aguirre, Paul Ceballos, H. Tohme, Joseph M. Beyer, Peter Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title | Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title_full | Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title_fullStr | Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title_full_unstemmed | Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title_short | Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W] |
| title_sort | provitamin a accumulation in cassava manihot esculenta roots driven by a single nucleotide polymorphism in a phytoene synthase gene w |
| topic | manihot esculenta varieties vitamins carotenoids plant breeding polymorphism retinol roots variedades vitaminas carotenoides mejoramiento de plantas polimorfismo raíces cell biology |
| url | https://hdl.handle.net/10568/43980 |
| work_keys_str_mv | AT welschr provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT arangojacobo provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT barc provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT salazarb provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT albabilis provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT beltrangiraldojorgealonso provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT chavarriagaaguirrepaul provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT ceballosh provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT tohmejosephm provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew AT beyerpeter provitaminaaccumulationincassavamanihotesculentarootsdrivenbyasinglenucleotidepolymorphisminaphytoenesynthasegenew |