Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America
Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ?-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with t...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Society for Microbiology
2005
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43977 |
| _version_ | 1855530262780182528 |
|---|---|
| author | Chen, W.M. Faria, S.M. de Straliotto, R Pitard, RM Simoes-Araujo, JL Chou, JH Chou, YJ Barrios, E. Prescott, AR Elliott, G.N. Sprent, JI Young, JPW James, EK |
| author_browse | Barrios, E. Chen, W.M. Chou, JH Chou, YJ Elliott, G.N. Faria, S.M. de James, EK Pitard, RM Prescott, AR Simoes-Araujo, JL Sprent, JI Straliotto, R Young, JPW |
| author_facet | Chen, W.M. Faria, S.M. de Straliotto, R Pitard, RM Simoes-Araujo, JL Chou, JH Chou, YJ Barrios, E. Prescott, AR Elliott, G.N. Sprent, JI Young, JPW James, EK |
| author_sort | Chen, W.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ?-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ?-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes. |
| format | Journal Article |
| id | CGSpace43977 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace439772024-08-27T10:36:23Z Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America Chen, W.M. Faria, S.M. de Straliotto, R Pitard, RM Simoes-Araujo, JL Chou, JH Chou, YJ Barrios, E. Prescott, AR Elliott, G.N. Sprent, JI Young, JPW James, EK feed crops symbiosis legumes rhizobium burkholderiaceae mimosa nitrogen fixation genes simbiósis leguminosas fijación del nitrógeno Twenty Mimosa-nodulating bacterial strains from Brazil and Venezuela, together with eight reference Mimosa-nodulating rhizobial strains and two other ?-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns and formed a single cluster together with the known ?-rhizobia, Burkholderia caribensis, Burkholderia phymatum, and Burkholderia tuberum. The 16S rRNA gene sequences of 15 of the 20 strains were determined, and all were shown to belong to the genus Burkholderia; four distinct clusters could be discerned, with strains isolated from the same host species usually clustering very closely. Five of the strains (MAP3-5, Br3407, Br3454, Br3461, and Br3469) were selected for further studies of the symbiosis-related genes nodA, the NodD-dependent regulatory consensus sequences (nod box), and nifH. The nodA and nifH sequences were very close to each other and to those of B. phymatum STM815, B. caribensis TJ182, and Cupriavidus taiwanensis LMG19424 but were relatively distant from those of B. tuberum STM678. In addition to nodulating their original hosts, all five strains could also nodulate other Mimosa spp., and all produced nodules on Mimosa pudica that had nitrogenase (acetylene reduction) activities and structures typical of effective N2-fixing symbioses. Finally, both wild-type and green fluorescent protein-expressing transconjugant strains of Br3461 and MAP3-5 produced N2-fixing nodules on their original hosts, Mimosa bimucronata (Br3461) and Mimosa pigra (MAP3-5), and hence this confirms strongly that Burkholderia strains can form effective symbioses with legumes. 2005-11 2014-10-02T08:33:02Z 2014-10-02T08:33:02Z Journal Article https://hdl.handle.net/10568/43977 en Open Access American Society for Microbiology |
| spellingShingle | feed crops symbiosis legumes rhizobium burkholderiaceae mimosa nitrogen fixation genes simbiósis leguminosas fijación del nitrógeno Chen, W.M. Faria, S.M. de Straliotto, R Pitard, RM Simoes-Araujo, JL Chou, JH Chou, YJ Barrios, E. Prescott, AR Elliott, G.N. Sprent, JI Young, JPW James, EK Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title | Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title_full | Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title_fullStr | Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title_full_unstemmed | Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title_short | Proof that Burkholderia strains form effective symbioses with legumes : A study of novel Mimosa-nodulating strains from South America |
| title_sort | proof that burkholderia strains form effective symbioses with legumes a study of novel mimosa nodulating strains from south america |
| topic | feed crops symbiosis legumes rhizobium burkholderiaceae mimosa nitrogen fixation genes simbiósis leguminosas fijación del nitrógeno |
| url | https://hdl.handle.net/10568/43977 |
| work_keys_str_mv | AT chenwm proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT fariasmde proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT straliottor proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT pitardrm proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT simoesaraujojl proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT choujh proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT chouyj proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT barriose proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT prescottar proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT elliottgn proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT sprentji proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT youngjpw proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica AT jamesek proofthatburkholderiastrainsformeffectivesymbioseswithlegumesastudyofnovelmimosanodulatingstrainsfromsouthamerica |