Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya
This study focused on the isolation, identification (sequencing of 16S rDNA gene) and determination of the phosphorus (P)-solubilizing efficiency of native populations of phosphate-solubilizing bacteria (PSB) in 13 Kenyan soils with differing chemical characteristics. Air-dried soil samples were ser...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2012
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| Online Access: | https://hdl.handle.net/10568/43905 |
| _version_ | 1855535646081286144 |
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| author | Ndung'u-Magiroi, Keziah Wairimu Herselman, L. Okalebo, JR Othieno, CO Pypers, Pieter Lesueur, Didier |
| author_browse | Herselman, L. Lesueur, Didier Ndung'u-Magiroi, Keziah Wairimu Okalebo, JR Othieno, CO Pypers, Pieter |
| author_facet | Ndung'u-Magiroi, Keziah Wairimu Herselman, L. Okalebo, JR Othieno, CO Pypers, Pieter Lesueur, Didier |
| author_sort | Ndung'u-Magiroi, Keziah Wairimu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This study focused on the isolation, identification (sequencing of 16S rDNA gene) and determination of the phosphorus (P)-solubilizing efficiency of native populations of phosphate-solubilizing bacteria (PSB) in 13 Kenyan soils with differing chemical characteristics. Air-dried soil samples were serially diluted and plated on NBRIP media and enumerated. Their phosphate-solubilizing efficiency was assessed on Frioni s agar. Pearson correlation coefficients were determined between PSB populations and soil properties. The PSB populations varied among the sites tested and had a positive and significant correlation (p???0.05) with organic carbon (r?=?0.76), exchangeable calcium (r?=?0.93) and exchangeable magnesium (r?=?0.92). A total of 150 isolates were identified to the genus and species level. Among the isolates, Bacillus megaterium, Bacillus sp. and Arthrobacter sp. were the most abundant and well-distributed strains. However, only 5% of the total isolates were efficient in terms of phosphate-solubilizing efficiency. The results indicate that although there were many PSB strains in the soils tested, only a few (5%) were effective in terms of their phosphate-solubilizing ability. It is therefore unlikely that native PSB contribute significantly to solubilizing phosphate in the soils tested, which would ultimately benefit plant growth. Therefore, inoculation with effective strains with a high P solubilization potential is necessary. |
| format | Journal Article |
| id | CGSpace43905 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace439052024-08-27T10:36:50Z Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya Ndung'u-Magiroi, Keziah Wairimu Herselman, L. Okalebo, JR Othieno, CO Pypers, Pieter Lesueur, Didier This study focused on the isolation, identification (sequencing of 16S rDNA gene) and determination of the phosphorus (P)-solubilizing efficiency of native populations of phosphate-solubilizing bacteria (PSB) in 13 Kenyan soils with differing chemical characteristics. Air-dried soil samples were serially diluted and plated on NBRIP media and enumerated. Their phosphate-solubilizing efficiency was assessed on Frioni s agar. Pearson correlation coefficients were determined between PSB populations and soil properties. The PSB populations varied among the sites tested and had a positive and significant correlation (p???0.05) with organic carbon (r?=?0.76), exchangeable calcium (r?=?0.93) and exchangeable magnesium (r?=?0.92). A total of 150 isolates were identified to the genus and species level. Among the isolates, Bacillus megaterium, Bacillus sp. and Arthrobacter sp. were the most abundant and well-distributed strains. However, only 5% of the total isolates were efficient in terms of phosphate-solubilizing efficiency. The results indicate that although there were many PSB strains in the soils tested, only a few (5%) were effective in terms of their phosphate-solubilizing ability. It is therefore unlikely that native PSB contribute significantly to solubilizing phosphate in the soils tested, which would ultimately benefit plant growth. Therefore, inoculation with effective strains with a high P solubilization potential is necessary. 2012-09 2014-10-02T08:32:55Z 2014-10-02T08:32:55Z Journal Article https://hdl.handle.net/10568/43905 en Open Access Springer |
| spellingShingle | Ndung'u-Magiroi, Keziah Wairimu Herselman, L. Okalebo, JR Othieno, CO Pypers, Pieter Lesueur, Didier Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title | Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title_full | Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title_fullStr | Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title_full_unstemmed | Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title_short | Occurrence and genetic diversity of phosphate-solubilizing bacteria in soils of differing chemical characteristics in Kenya |
| title_sort | occurrence and genetic diversity of phosphate solubilizing bacteria in soils of differing chemical characteristics in kenya |
| url | https://hdl.handle.net/10568/43905 |
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