Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola
Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Af...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2002
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43878 |
| _version_ | 1855541728296042496 |
|---|---|
| author | Mahuku, George S. Henriquez Naranjo, MA Muñoz, J. Buruchara, Robin Arani |
| author_browse | Buruchara, Robin Arani Henriquez Naranjo, MA Mahuku, George S. Muñoz, J. |
| author_facet | Mahuku, George S. Henriquez Naranjo, MA Muñoz, J. Buruchara, Robin Arani |
| author_sort | Mahuku, George S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Africa has identified a group of isolates classified as Andean using random amplified polymorphic DNA (RAPD), but which are able to infect some Mesoamerican differential varieties. These isolates, designated Afro-Andean, have been identified only in Africa. Random amplified microsatellites, RAPD, and restriction digestion of amplified ribosomal intergenic spacer region were used to elucidate the relationships among the Afro-Andean, Andean, and Mesoamerican groups of P. griseola. Cluster and multiple correspondence analysis of molecular data separated isolates into Andean and Meso-american groups, and the Afro-Andean isolates clustered with Andean isolates. Analysis of molecular variance ascribed 2.8% of the total genetic variation to differences between Afro-Andean and Andean isolates from Africa. Gene diversity analysis revealed no genetic differentiation (GST = 0.004) between Afro-Andean and Andean isolates from Africa. However, significant levels of genetic differentiation (GST = 0.39) were observed between Afro-Andean or Andean isolates from Africa and Andean isolates from Latin America, revealing significant geographical differentiation within the Andean lineage. Results from this study showed that Afro-Andean isolates do not constitute a new P. griseola group and do not represent long-term evolution of the pathogen genome, but rather are likely the consequents of point mutations in genes for virulence. This finding has significant implications in the deployment of resistant bean genotypes. |
| format | Journal Article |
| id | CGSpace43878 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace438782025-03-11T12:14:31Z Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola Mahuku, George S. Henriquez Naranjo, MA Muñoz, J. Buruchara, Robin Arani phaseolus vulgaris phaeoisariopsis griseola genetic variation genetic markers pcr provenance variación genética marcadores genéticos procedencia Coevolution of the angular leaf spot pathogen, Phaeoisariopsis griseola, with its common bean host has been demonstrated, and P. griseola isolates have been divided into Andean and Mesoamerican groups that correspond to defined bean gene pools. Recent characterization of P. griseola isolates from Africa has identified a group of isolates classified as Andean using random amplified polymorphic DNA (RAPD), but which are able to infect some Mesoamerican differential varieties. These isolates, designated Afro-Andean, have been identified only in Africa. Random amplified microsatellites, RAPD, and restriction digestion of amplified ribosomal intergenic spacer region were used to elucidate the relationships among the Afro-Andean, Andean, and Mesoamerican groups of P. griseola. Cluster and multiple correspondence analysis of molecular data separated isolates into Andean and Meso-american groups, and the Afro-Andean isolates clustered with Andean isolates. Analysis of molecular variance ascribed 2.8% of the total genetic variation to differences between Afro-Andean and Andean isolates from Africa. Gene diversity analysis revealed no genetic differentiation (GST = 0.004) between Afro-Andean and Andean isolates from Africa. However, significant levels of genetic differentiation (GST = 0.39) were observed between Afro-Andean or Andean isolates from Africa and Andean isolates from Latin America, revealing significant geographical differentiation within the Andean lineage. Results from this study showed that Afro-Andean isolates do not constitute a new P. griseola group and do not represent long-term evolution of the pathogen genome, but rather are likely the consequents of point mutations in genes for virulence. This finding has significant implications in the deployment of resistant bean genotypes. 2002-06 2014-10-02T08:32:52Z 2014-10-02T08:32:52Z Journal Article https://hdl.handle.net/10568/43878 en Open Access Scientific Societies Mahuku; George S.; Henriquez; María Antonia; Muñoz; Jaime; Buruchara; Robin (2002). Molecular Markers Dispute the Existence of the Afro-Andean Group of the Bean Angular Leaf Spot Pathogen, Phaeoisariopsis griseola. Phytopathology 92 (6): 580-589 |
| spellingShingle | phaseolus vulgaris phaeoisariopsis griseola genetic variation genetic markers pcr provenance variación genética marcadores genéticos procedencia Mahuku, George S. Henriquez Naranjo, MA Muñoz, J. Buruchara, Robin Arani Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title | Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title_full | Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title_fullStr | Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title_full_unstemmed | Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title_short | Molecular markers dispute the existence of the Afro-Andean group of the bean angular leaf spot pathogen, Phaeoisariopsis griseola |
| title_sort | molecular markers dispute the existence of the afro andean group of the bean angular leaf spot pathogen phaeoisariopsis griseola |
| topic | phaseolus vulgaris phaeoisariopsis griseola genetic variation genetic markers pcr provenance variación genética marcadores genéticos procedencia |
| url | https://hdl.handle.net/10568/43878 |
| work_keys_str_mv | AT mahukugeorges molecularmarkersdisputetheexistenceoftheafroandeangroupofthebeanangularleafspotpathogenphaeoisariopsisgriseola AT henriqueznaranjoma molecularmarkersdisputetheexistenceoftheafroandeangroupofthebeanangularleafspotpathogenphaeoisariopsisgriseola AT munozj molecularmarkersdisputetheexistenceoftheafroandeangroupofthebeanangularleafspotpathogenphaeoisariopsisgriseola AT buruchararobinarani molecularmarkersdisputetheexistenceoftheafroandeangroupofthebeanangularleafspotpathogenphaeoisariopsisgriseola |