Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola
Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: riboty...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
1998
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43871 |
| _version_ | 1855520068321935360 |
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| author | Verdier, Valerie M. Assigbétsé, K. Khatri-Chhetri, G. Wydra, K. Ruldoph, K Geiger, JP |
| author_browse | Assigbétsé, K. Geiger, JP Khatri-Chhetri, G. Ruldoph, K Verdier, Valerie M. Wydra, K. |
| author_facet | Verdier, Valerie M. Assigbétsé, K. Khatri-Chhetri, G. Wydra, K. Ruldoph, K Geiger, JP |
| author_sort | Verdier, Valerie M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: ribotyping with EcoRI endonuclease, and RFLP analysis with a plasmid probe (pthB) containing a gene required for pathogenicity from Xanthomonas campestris pv. manihotis. Ribotyping revealed a unique pattern for all the strains that corresponded to the previously described ribotype rRNA7. Based on polymorphism detected by pthB among Xcv strains, nine haplotypes were defined. The observed genetic variation was independent of the geographic origin of the strains and of pathogenic variation. Some haplotypes were widely distributed, whereas others were localized. In some cases, we could differentiate strains isolated from blight symptoms and pustules according to haplotypic composition. However, in most cases, no significant differences were observed. Our results and the previous pathogenic and biochemical characterizations suggest that the strains isolated from leaves with blight symptoms or minute pustules belong to the same pathovar. We provide information on pathogen diversity that can be used to identify and characterize resistant germplasm. |
| format | Journal Article |
| id | CGSpace43871 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace438712025-01-27T15:00:52Z Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola Verdier, Valerie M. Assigbétsé, K. Khatri-Chhetri, G. Wydra, K. Ruldoph, K Geiger, JP feed crops vigna unguiculata cowpeas xanthomonas campestris rflp dna pathogenicity caupí adn patogenicidad horticulture pests diseases Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: ribotyping with EcoRI endonuclease, and RFLP analysis with a plasmid probe (pthB) containing a gene required for pathogenicity from Xanthomonas campestris pv. manihotis. Ribotyping revealed a unique pattern for all the strains that corresponded to the previously described ribotype rRNA7. Based on polymorphism detected by pthB among Xcv strains, nine haplotypes were defined. The observed genetic variation was independent of the geographic origin of the strains and of pathogenic variation. Some haplotypes were widely distributed, whereas others were localized. In some cases, we could differentiate strains isolated from blight symptoms and pustules according to haplotypic composition. However, in most cases, no significant differences were observed. Our results and the previous pathogenic and biochemical characterizations suggest that the strains isolated from leaves with blight symptoms or minute pustules belong to the same pathovar. We provide information on pathogen diversity that can be used to identify and characterize resistant germplasm. 1998-08 2014-10-02T08:32:51Z 2014-10-02T08:32:51Z Journal Article https://hdl.handle.net/10568/43871 en Limited Access Springer Verdier, V., Assigbétsé, K., Khatri-Chhetri, G., Wydra, K., Rudolph, K., & Geiger, J.-P. (1998). European Journal of Plant Pathology, 104(6), 595–602. https://doi.org/10.1023/a:1008610517437 |
| spellingShingle | feed crops vigna unguiculata cowpeas xanthomonas campestris rflp dna pathogenicity caupí adn patogenicidad horticulture pests diseases Verdier, Valerie M. Assigbétsé, K. Khatri-Chhetri, G. Wydra, K. Ruldoph, K Geiger, JP Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title | Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title_full | Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title_fullStr | Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title_full_unstemmed | Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title_short | Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola |
| title_sort | molecular characterization of the incitant of cowpea bacterial blight and pustule xanthomonas campestris pv vignicola |
| topic | feed crops vigna unguiculata cowpeas xanthomonas campestris rflp dna pathogenicity caupí adn patogenicidad horticulture pests diseases |
| url | https://hdl.handle.net/10568/43871 |
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