Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla

Random amplified polymorphic DNA (RAPD) markers were used for assessing genetic diversity and its structure in a collection of the drought-tolerant, tropical multipurpose shrub legume Flemingia macrophylla (Willd.) Merrill. The species is especially suited to low-input smallholder production systems...

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Main Authors: Andersson, Meike S., Peters, Michael, Schultze-Kraft, Rainer, Gallego Sánchez, Gerardo J., Duque E., Myriam Cristina
Format: Journal Article
Language:Inglés
Published: Springer 2006
Subjects:
Online Access:https://hdl.handle.net/10568/43870
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author Andersson, Meike S.
Peters, Michael
Schultze-Kraft, Rainer
Gallego Sánchez, Gerardo J.
Duque E., Myriam Cristina
author_browse Andersson, Meike S.
Duque E., Myriam Cristina
Gallego Sánchez, Gerardo J.
Peters, Michael
Schultze-Kraft, Rainer
author_facet Andersson, Meike S.
Peters, Michael
Schultze-Kraft, Rainer
Gallego Sánchez, Gerardo J.
Duque E., Myriam Cristina
author_sort Andersson, Meike S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Random amplified polymorphic DNA (RAPD) markers were used for assessing genetic diversity and its structure in a collection of the drought-tolerant, tropical multipurpose shrub legume Flemingia macrophylla (Willd.) Merrill. The species is especially suited to low-input smallholder production systems and is used as dry season forage supplement, live soil cover, mulch and living barrier, among others. Genetic groups identified by RAPD analysis related closely to four morphotypes revealed by multivariate analysis of morphological, agronomic and forage quality characteristics. Overall genetic diversity in the collection was moderate (H T = 0.241), with 79% differentiation among and high genetic similarity (GS) within groups (0.672 0.965). Results indicate a closer relationship of the semi-erect morphotypes M3 and M4 with F. stricta rather than with the erect morphotype M1. The latter was genetically the most depauperate, followed by morphotypes M2 and M4. New evidence is provided that non-Asian accessions collected in Africa and South America are derived from introduced Southeast Asian material. Furthermore, RAPD data suggest the presence of duplicates in the collection, especially in the overrepresented erect morphotype M1.
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spelling CGSpace438702024-08-27T10:37:26Z Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla Andersson, Meike S. Peters, Michael Schultze-Kraft, Rainer Gallego Sánchez, Gerardo J. Duque E., Myriam Cristina feed crops flemingia macrophylla germplasm collections biodiversity genetic markers rapd geographical distribution plant anatomy colecciones de material genético biodiversidad marcadores genéticos distribución geográfica anatomía de la planta Random amplified polymorphic DNA (RAPD) markers were used for assessing genetic diversity and its structure in a collection of the drought-tolerant, tropical multipurpose shrub legume Flemingia macrophylla (Willd.) Merrill. The species is especially suited to low-input smallholder production systems and is used as dry season forage supplement, live soil cover, mulch and living barrier, among others. Genetic groups identified by RAPD analysis related closely to four morphotypes revealed by multivariate analysis of morphological, agronomic and forage quality characteristics. Overall genetic diversity in the collection was moderate (H T = 0.241), with 79% differentiation among and high genetic similarity (GS) within groups (0.672 0.965). Results indicate a closer relationship of the semi-erect morphotypes M3 and M4 with F. stricta rather than with the erect morphotype M1. The latter was genetically the most depauperate, followed by morphotypes M2 and M4. New evidence is provided that non-Asian accessions collected in Africa and South America are derived from introduced Southeast Asian material. Furthermore, RAPD data suggest the presence of duplicates in the collection, especially in the overrepresented erect morphotype M1. 2006-09-25 2014-10-02T08:32:51Z 2014-10-02T08:32:51Z Journal Article https://hdl.handle.net/10568/43870 en Limited Access Springer
spellingShingle feed crops
flemingia macrophylla
germplasm collections
biodiversity
genetic markers
rapd
geographical distribution
plant anatomy
colecciones de material genético
biodiversidad
marcadores genéticos
distribución geográfica
anatomía de la planta
Andersson, Meike S.
Peters, Michael
Schultze-Kraft, Rainer
Gallego Sánchez, Gerardo J.
Duque E., Myriam Cristina
Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title_full Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title_fullStr Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title_full_unstemmed Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title_short Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla
title_sort molecular characterization of a collection of the tropical multipurpose shrub legume flemingia macrophylla
topic feed crops
flemingia macrophylla
germplasm collections
biodiversity
genetic markers
rapd
geographical distribution
plant anatomy
colecciones de material genético
biodiversidad
marcadores genéticos
distribución geográfica
anatomía de la planta
url https://hdl.handle.net/10568/43870
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