Molecular and phenotypic diversity of common bean landraces from Nicaragua

The knowledge and understanding of the genetic structure of bean (Phaseolus vulgaris L.) landraces is important for the implementation of measures addressed to their management and conservation. The purpose of this paper was to study the pattern of genetic variation in nine red-seeded landraces curr...

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Autores principales: Gómez, O.J., Blair, Matthew W., Frankow-Lindberg, BE, Gullberg, U.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2004
Materias:
Acceso en línea:https://hdl.handle.net/10568/43866
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author Gómez, O.J.
Blair, Matthew W.
Frankow-Lindberg, BE
Gullberg, U.
author_browse Blair, Matthew W.
Frankow-Lindberg, BE
Gullberg, U.
Gómez, O.J.
author_facet Gómez, O.J.
Blair, Matthew W.
Frankow-Lindberg, BE
Gullberg, U.
author_sort Gómez, O.J.
collection Repository of Agricultural Research Outputs (CGSpace)
description The knowledge and understanding of the genetic structure of bean (Phaseolus vulgaris L.) landraces is important for the implementation of measures addressed to their management and conservation. The purpose of this paper was to study the pattern of genetic variation in nine red-seeded landraces currently grown by farmers with molecular and phenotypic markers. Twelve individuals per landrace were genotyped with seven bean microsatellite markers. Fourteen phenotypic traits were additionally measured in a field study in two localities. An important finding of this study was the complementary information obtained with both kinds of markers. Most of the variation at the molecular level was explained by differences within or among landraces but not among agroecological zones, while at the phenotypic level most of the variation was attributed to differences among agroecological zones. This suggests that molecular differentiation of landraces [coancestry coefficient (F ST) = 0.34] was due to founder effect while phenotypic differentiation was due to the effect of adaptation. Within landraces, an average of 5.7 alleles per locus was identified, with a range from 2 to 13 alleles depending on the individual microsatellite. The average gene diversity within landraces and total gene diversity was 0.35 and 0.51, respectively. Implications of the findings in planning future collections of genetic resources of common bean as well as the effect of sites on uncovering adaptive traits are also discussed.
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spelling CGSpace438662025-11-12T05:52:55Z Molecular and phenotypic diversity of common bean landraces from Nicaragua Gómez, O.J. Blair, Matthew W. Frankow-Lindberg, BE Gullberg, U. phaseolus vulgaris varieties genetic variation genetic markers phenotypes pcr microsatellites variedades variación genética marcadores genéticos fenotipos microsatélites The knowledge and understanding of the genetic structure of bean (Phaseolus vulgaris L.) landraces is important for the implementation of measures addressed to their management and conservation. The purpose of this paper was to study the pattern of genetic variation in nine red-seeded landraces currently grown by farmers with molecular and phenotypic markers. Twelve individuals per landrace were genotyped with seven bean microsatellite markers. Fourteen phenotypic traits were additionally measured in a field study in two localities. An important finding of this study was the complementary information obtained with both kinds of markers. Most of the variation at the molecular level was explained by differences within or among landraces but not among agroecological zones, while at the phenotypic level most of the variation was attributed to differences among agroecological zones. This suggests that molecular differentiation of landraces [coancestry coefficient (F ST) = 0.34] was due to founder effect while phenotypic differentiation was due to the effect of adaptation. Within landraces, an average of 5.7 alleles per locus was identified, with a range from 2 to 13 alleles depending on the individual microsatellite. The average gene diversity within landraces and total gene diversity was 0.35 and 0.51, respectively. Implications of the findings in planning future collections of genetic resources of common bean as well as the effect of sites on uncovering adaptive traits are also discussed. 2004 2014-10-02T08:32:51Z 2014-10-02T08:32:51Z Journal Article https://hdl.handle.net/10568/43866 en Open Access application/pdf
spellingShingle phaseolus vulgaris
varieties
genetic variation
genetic markers
phenotypes
pcr
microsatellites
variedades
variación genética
marcadores genéticos
fenotipos
microsatélites
Gómez, O.J.
Blair, Matthew W.
Frankow-Lindberg, BE
Gullberg, U.
Molecular and phenotypic diversity of common bean landraces from Nicaragua
title Molecular and phenotypic diversity of common bean landraces from Nicaragua
title_full Molecular and phenotypic diversity of common bean landraces from Nicaragua
title_fullStr Molecular and phenotypic diversity of common bean landraces from Nicaragua
title_full_unstemmed Molecular and phenotypic diversity of common bean landraces from Nicaragua
title_short Molecular and phenotypic diversity of common bean landraces from Nicaragua
title_sort molecular and phenotypic diversity of common bean landraces from nicaragua
topic phaseolus vulgaris
varieties
genetic variation
genetic markers
phenotypes
pcr
microsatellites
variedades
variación genética
marcadores genéticos
fenotipos
microsatélites
url https://hdl.handle.net/10568/43866
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