Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta
Cassava (Manihot esculenta Crantz) is a major root crop widely grown in the tropics. Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in Latin America and Africa resulting in significant losses. The preferred control method is the use of resista...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Canadian Science Publishing
2007
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43838 |
| _version_ | 1855534685639147520 |
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| author | López, C.E. Quesada Ocampo, LM Bohórquez Cháux, Adriana Duque E., Myriam Cristina Vargas Daza, Jaime Tohme, Joseph M. Verdier, Valerie M. |
| author_browse | Bohórquez Cháux, Adriana Duque E., Myriam Cristina López, C.E. Quesada Ocampo, LM Tohme, Joseph M. Vargas Daza, Jaime Verdier, Valerie M. |
| author_facet | López, C.E. Quesada Ocampo, LM Bohórquez Cháux, Adriana Duque E., Myriam Cristina Vargas Daza, Jaime Tohme, Joseph M. Verdier, Valerie M. |
| author_sort | López, C.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is a major root crop widely grown in the tropics. Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in Latin America and Africa resulting in significant losses. The preferred control method is the use of resistant genotypes. Mapping expressed sequence tags (ESTs) and determining their co-localization with quantitative trait loci (QTLs) may give additional evidence of the role of the corresponding genes in resistance or defense. Twenty-one EST-derived simple sequence repeats (SSRs) were mapped in 16 linkage groups. ESTs showing similarities with candidate resistance genes or defense genes were also mapped using strategies such as restriction fragment length polymorphisms, cleaved amplified polymorphic sequences, and allele-specific primers. In total, 10 defense-related genes and 2 bacterial artificial chromosomes (BACs) containing resistance gene candidates (RGCs) were mapped in 11 linkage groups. Two new QTLs associated with resistance to Xam strains CIO121 and CIO151 were detected in linkage groups A and U, respectively. The QTL in linkage group U explained 61.6% of the phenotypic variance and was associated with an RGC-containing BAC. No correlation was found between the new EST-derived SSRs or other mapped ESTs and the new or previously reported QTLs. |
| format | Journal Article |
| id | CGSpace43838 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace438382024-08-27T10:36:44Z Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta López, C.E. Quesada Ocampo, LM Bohórquez Cháux, Adriana Duque E., Myriam Cristina Vargas Daza, Jaime Tohme, Joseph M. Verdier, Valerie M. manihot esculenta chromosome mapping quantitative trait loci genetic markers genetic resistance cassava bacterial blight mapeo de cromosomas loci de rasgos cuantitativos marcadores genéticos resistencia genética anublo bacteriano de la yuca Cassava (Manihot esculenta Crantz) is a major root crop widely grown in the tropics. Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in Latin America and Africa resulting in significant losses. The preferred control method is the use of resistant genotypes. Mapping expressed sequence tags (ESTs) and determining their co-localization with quantitative trait loci (QTLs) may give additional evidence of the role of the corresponding genes in resistance or defense. Twenty-one EST-derived simple sequence repeats (SSRs) were mapped in 16 linkage groups. ESTs showing similarities with candidate resistance genes or defense genes were also mapped using strategies such as restriction fragment length polymorphisms, cleaved amplified polymorphic sequences, and allele-specific primers. In total, 10 defense-related genes and 2 bacterial artificial chromosomes (BACs) containing resistance gene candidates (RGCs) were mapped in 11 linkage groups. Two new QTLs associated with resistance to Xam strains CIO121 and CIO151 were detected in linkage groups A and U, respectively. The QTL in linkage group U explained 61.6% of the phenotypic variance and was associated with an RGC-containing BAC. No correlation was found between the new EST-derived SSRs or other mapped ESTs and the new or previously reported QTLs. 2007-12 2014-10-02T08:32:48Z 2014-10-02T08:32:48Z Journal Article https://hdl.handle.net/10568/43838 en Open Access Canadian Science Publishing |
| spellingShingle | manihot esculenta chromosome mapping quantitative trait loci genetic markers genetic resistance cassava bacterial blight mapeo de cromosomas loci de rasgos cuantitativos marcadores genéticos resistencia genética anublo bacteriano de la yuca López, C.E. Quesada Ocampo, LM Bohórquez Cháux, Adriana Duque E., Myriam Cristina Vargas Daza, Jaime Tohme, Joseph M. Verdier, Valerie M. Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title | Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title_full | Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title_fullStr | Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title_full_unstemmed | Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title_short | Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta |
| title_sort | mapping est derived ssrs and ests involved in resistance to bacterial blight in manihot esculenta |
| topic | manihot esculenta chromosome mapping quantitative trait loci genetic markers genetic resistance cassava bacterial blight mapeo de cromosomas loci de rasgos cuantitativos marcadores genéticos resistencia genética anublo bacteriano de la yuca |
| url | https://hdl.handle.net/10568/43838 |
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