African trypanosomiasis resistance in cattle by a transgenic approach
African trypanosomiasis, caused by extracellular protozoan parasites (Trypanosoma), is, a major disease in cattle that affects agricultural production in broad regions of Africa. The, parasites are transmitted between mammals by infected tsetse flies (Glossina sp.) during, blood feeding. Both wild a...
| Autores principales: | , , , , , |
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| Formato: | Póster |
| Lenguaje: | Inglés |
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International Livestock Research Institute
2014
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43796 |
| _version_ | 1855541109835431936 |
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| author | Mingyan Yu Muteti, C. Ogugo, M. Ritchie, W.A. Raper, J. Kemp, Stephen J. |
| author_browse | Kemp, Stephen J. Mingyan Yu Muteti, C. Ogugo, M. Raper, J. Ritchie, W.A. |
| author_facet | Mingyan Yu Muteti, C. Ogugo, M. Ritchie, W.A. Raper, J. Kemp, Stephen J. |
| author_sort | Mingyan Yu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African trypanosomiasis, caused by extracellular protozoan parasites (Trypanosoma), is, a major disease in cattle that affects agricultural production in broad regions of Africa. The, parasites are transmitted between mammals by infected tsetse flies (Glossina sp.) during, blood feeding. Both wild and domestic animals are potential reservoirs of the parasites for, human infection resulting in human sleeping sickness. In order to control the disease, we, proposed a new strategy for creating resistance in cattle to African trypanosomiasis by a, transgenic approach. Using the technique of somatic cell nuclear transfer (cloning), we aim, to establish genetically modified cattle on the background of a Kenyan indigenous breed –, Kenyan Boran, which carry a gene that imparts resistance to African trypanosomes. The, gene, apoL-1, encodes the key trypanolytic component of baboon’s protective Trypanosome, Lytic Factor (TLF) against both cattle and human infective trypanosomes. TLFs are only, found in humans, gorillas, sooty mangabys, mandrills and baboons and govern resistance, to different African trypanosome species. Baboons are remarkably resistant to all African, trypanosomes due to its TLF, specifically apoL-1. Previous research with transgenic mice, has shown that the baboon apoL-1 product was able to confer protection to the mice, against trypanosome infection. Therefore, we hypothesise that expression of baboon apoL-, 1 in cattle will also endow endogenous resistance to trypanosomes. As the proof of concept, step, we have successfully set up and tested the platform for somatic cell nuclear transfer, using Boran bovine embryonic fibroblasts (BEFs). In total, two cloned calves were born by, caesarean section operation. One calf survives up to today and is in good health. Attempts, are ongoing to introduce the apoL-1 gene into two of the BEFs lines for future production, of transgenic cattle., |
| format | Poster |
| id | CGSpace43796 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | International Livestock Research Institute |
| publisherStr | International Livestock Research Institute |
| record_format | dspace |
| spelling | CGSpace437962025-11-04T18:06:22Z African trypanosomiasis resistance in cattle by a transgenic approach Mingyan Yu Muteti, C. Ogugo, M. Ritchie, W.A. Raper, J. Kemp, Stephen J. trypanosomiasis cattle animal diseases African trypanosomiasis, caused by extracellular protozoan parasites (Trypanosoma), is, a major disease in cattle that affects agricultural production in broad regions of Africa. The, parasites are transmitted between mammals by infected tsetse flies (Glossina sp.) during, blood feeding. Both wild and domestic animals are potential reservoirs of the parasites for, human infection resulting in human sleeping sickness. In order to control the disease, we, proposed a new strategy for creating resistance in cattle to African trypanosomiasis by a, transgenic approach. Using the technique of somatic cell nuclear transfer (cloning), we aim, to establish genetically modified cattle on the background of a Kenyan indigenous breed –, Kenyan Boran, which carry a gene that imparts resistance to African trypanosomes. The, gene, apoL-1, encodes the key trypanolytic component of baboon’s protective Trypanosome, Lytic Factor (TLF) against both cattle and human infective trypanosomes. TLFs are only, found in humans, gorillas, sooty mangabys, mandrills and baboons and govern resistance, to different African trypanosome species. Baboons are remarkably resistant to all African, trypanosomes due to its TLF, specifically apoL-1. Previous research with transgenic mice, has shown that the baboon apoL-1 product was able to confer protection to the mice, against trypanosome infection. Therefore, we hypothesise that expression of baboon apoL-, 1 in cattle will also endow endogenous resistance to trypanosomes. As the proof of concept, step, we have successfully set up and tested the platform for somatic cell nuclear transfer, using Boran bovine embryonic fibroblasts (BEFs). In total, two cloned calves were born by, caesarean section operation. One calf survives up to today and is in good health. Attempts, are ongoing to introduce the apoL-1 gene into two of the BEFs lines for future production, of transgenic cattle., 2014-09-17 2014-09-30T10:47:30Z 2014-09-30T10:47:30Z Poster https://hdl.handle.net/10568/43796 en http://www.tropentag.de/2014/abstracts/links/Yu_HmboDUHW.pdf Open Access application/pdf International Livestock Research Institute Mingyan Yu, Muteti, C., Ogugo, M., Ritchie, W.A., Raper, J. and Kemp, S. 2014. African trypanosomiasis resistance in cattle by a transgenic approach. Poster prepared for the Tropentag 2014 Conference on Bridging the Gap between Increasing Knowledge and Decreasing Resources, Prague, 17-19 September 2014. Nairobi, Kenya: ILRI. |
| spellingShingle | trypanosomiasis cattle animal diseases Mingyan Yu Muteti, C. Ogugo, M. Ritchie, W.A. Raper, J. Kemp, Stephen J. African trypanosomiasis resistance in cattle by a transgenic approach |
| title | African trypanosomiasis resistance in cattle by a transgenic approach |
| title_full | African trypanosomiasis resistance in cattle by a transgenic approach |
| title_fullStr | African trypanosomiasis resistance in cattle by a transgenic approach |
| title_full_unstemmed | African trypanosomiasis resistance in cattle by a transgenic approach |
| title_short | African trypanosomiasis resistance in cattle by a transgenic approach |
| title_sort | african trypanosomiasis resistance in cattle by a transgenic approach |
| topic | trypanosomiasis cattle animal diseases |
| url | https://hdl.handle.net/10568/43796 |
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