Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume
The degree of genetic diversity of 127 Colletotrichum gloeosporioides isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with nine arbitrary primers of 10 bases, and by restriction fragment length polymorphi...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
1999
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43488 |
| _version_ | 1855526638742142976 |
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| author | Kelemu, Segenet Skinner, DZ Badel, JL Moreno, CX Rodríguez, M.X. Fernandes, C.D. Charchar, Maria José Chakraborty, S. |
| author_browse | Badel, JL Chakraborty, S. Charchar, Maria José Fernandes, C.D. Kelemu, Segenet Moreno, CX Rodríguez, M.X. Skinner, DZ |
| author_facet | Kelemu, Segenet Skinner, DZ Badel, JL Moreno, CX Rodríguez, M.X. Fernandes, C.D. Charchar, Maria José Chakraborty, S. |
| author_sort | Kelemu, Segenet |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The degree of genetic diversity of 127 Colletotrichum gloeosporioides isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with nine arbitrary primers of 10 bases, and by restriction fragment length polymorphism (RFLP) with a non-LTR (long terminal repeats) retrotransposon DNA sequence. The RAPD products revealed scorable polymorphism among the isolates, and a total of 80 band positions were scored. Sixty-three of the 127 isolates were clustered into 13 distinct lineages usually correlating with geographic origin. Where isolates from various regions were clustered together, most had identical host genotype origin. The pathogen population sampled from Carimagua, Colombia, a long-time Stylosanthes breeding and selection site, with a savanna ecosystem, was highly diverse. A set of 12 S. guianensis genotype differentials was used to characterize pathogenic variability of 104 isolates and their virulence patterns were grouped into 57 pathotypes. However, when they were tested on four Australian differentials, they grouped into 11 pathotypes. As shown in previous studies, no strict correlations existed between genetic diversity measured by RAPD or RFLP, and pathotype defined by pathogenicity pattern on the differentials. Southern blot analysis of the 127 isolates revealed 23 hybridizing fragments, resulting in 41 fingerprint patterns among the 127 isolates. Relationships between RFLP and RAPD variables were examined using Spearman's Rank Correlation Coefficient, which showed that the two measures of genotypic variation are in agreement. |
| format | Journal Article |
| id | CGSpace43488 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace434882024-08-29T11:41:31Z Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume Kelemu, Segenet Skinner, DZ Badel, JL Moreno, CX Rodríguez, M.X. Fernandes, C.D. Charchar, Maria José Chakraborty, S. feed crops anthracnosis colletotrichum lindemuthianum pathotypes genetic variation stylosanthes guianensis antracnosis patotipos variación genética The degree of genetic diversity of 127 Colletotrichum gloeosporioides isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with nine arbitrary primers of 10 bases, and by restriction fragment length polymorphism (RFLP) with a non-LTR (long terminal repeats) retrotransposon DNA sequence. The RAPD products revealed scorable polymorphism among the isolates, and a total of 80 band positions were scored. Sixty-three of the 127 isolates were clustered into 13 distinct lineages usually correlating with geographic origin. Where isolates from various regions were clustered together, most had identical host genotype origin. The pathogen population sampled from Carimagua, Colombia, a long-time Stylosanthes breeding and selection site, with a savanna ecosystem, was highly diverse. A set of 12 S. guianensis genotype differentials was used to characterize pathogenic variability of 104 isolates and their virulence patterns were grouped into 57 pathotypes. However, when they were tested on four Australian differentials, they grouped into 11 pathotypes. As shown in previous studies, no strict correlations existed between genetic diversity measured by RAPD or RFLP, and pathotype defined by pathogenicity pattern on the differentials. Southern blot analysis of the 127 isolates revealed 23 hybridizing fragments, resulting in 41 fingerprint patterns among the 127 isolates. Relationships between RFLP and RAPD variables were examined using Spearman's Rank Correlation Coefficient, which showed that the two measures of genotypic variation are in agreement. 1999 2014-09-24T08:42:12Z 2014-09-24T08:42:12Z Journal Article https://hdl.handle.net/10568/43488 en Limited Access Springer |
| spellingShingle | feed crops anthracnosis colletotrichum lindemuthianum pathotypes genetic variation stylosanthes guianensis antracnosis patotipos variación genética Kelemu, Segenet Skinner, DZ Badel, JL Moreno, CX Rodríguez, M.X. Fernandes, C.D. Charchar, Maria José Chakraborty, S. Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title | Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title_full | Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title_fullStr | Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title_full_unstemmed | Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title_short | Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume |
| title_sort | genetic diversity in south american colletotrichum gloeosporioides isolates from stylosanthes guianensis a tropical forage legume |
| topic | feed crops anthracnosis colletotrichum lindemuthianum pathotypes genetic variation stylosanthes guianensis antracnosis patotipos variación genética |
| url | https://hdl.handle.net/10568/43488 |
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