Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba
The genetic structure of rice blast pathogen populations was studied using 149 and 107 monoconidial isolates from Los Palacios and Sur del Jíbaro rice production locations in the western and central regions of Cuba, respectively. These isolates were collected from 35 rice genotypes and total DNA fro...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43486 |
| _version_ | 1855526577358503936 |
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| author | Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Mora, L Duque E., Myriam Cristina Deus, J.E. Cornide, MT |
| author_browse | Cornide, MT Correa Victoria, Fernando J. Deus, J.E. Duque E., Myriam Cristina Escobar, F. Fuentes, J.L Mora, L |
| author_facet | Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Mora, L Duque E., Myriam Cristina Deus, J.E. Cornide, MT |
| author_sort | Fuentes, J.L |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The genetic structure of rice blast pathogen populations was studied using 149 and 107 monoconidial isolates from Los Palacios and Sur del Jíbaro rice production locations in the western and central regions of Cuba, respectively. These isolates were collected from 35 rice genotypes and total DNA from each isolate was digested with the EcoRl enzyme and probed with a dispersed Magnaporthe grisea repeated sequence (MGR586), which has been widely used in the population analysis of this fungus. MGR586 DNA fingerprinting groups were identified based on DNA restriction fragment length polymorphism similarities. This analysis allowed the identification of 134 haplotypes that were partitioned into four highly related genetic groups. The mean similarity between groups ranged from 75 to 85%. Group A was predominant in both locations and was composed of 84.7% of all monoconidiol isolations from 33 rice genotypes. Isolates from groups B, C and D were associated with one or a small number of very related varieties in the LP region. Genetic diversity estimates between and within the pathogen collection showed a wider diversity in pathogen populations at the western rice region. This analysis also suggested that the migration of this pathogen could have occurred from the western to central rice regions in Cubo. These results suggested that the specific features of the Cuban fungal populations would be particularly useful for selecting germplasm sources for more durable blast-resistance breeding. |
| format | Journal Article |
| id | CGSpace43486 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| record_format | dspace |
| spelling | CGSpace434862025-05-15T09:40:26Z Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Mora, L Duque E., Myriam Cristina Deus, J.E. Cornide, MT oryza sativa rice genetic variation pyricularia dna fingerprinting arroz variación genética huellas genéticas adn The genetic structure of rice blast pathogen populations was studied using 149 and 107 monoconidial isolates from Los Palacios and Sur del Jíbaro rice production locations in the western and central regions of Cuba, respectively. These isolates were collected from 35 rice genotypes and total DNA from each isolate was digested with the EcoRl enzyme and probed with a dispersed Magnaporthe grisea repeated sequence (MGR586), which has been widely used in the population analysis of this fungus. MGR586 DNA fingerprinting groups were identified based on DNA restriction fragment length polymorphism similarities. This analysis allowed the identification of 134 haplotypes that were partitioned into four highly related genetic groups. The mean similarity between groups ranged from 75 to 85%. Group A was predominant in both locations and was composed of 84.7% of all monoconidiol isolations from 33 rice genotypes. Isolates from groups B, C and D were associated with one or a small number of very related varieties in the LP region. Genetic diversity estimates between and within the pathogen collection showed a wider diversity in pathogen populations at the western rice region. This analysis also suggested that the migration of this pathogen could have occurred from the western to central rice regions in Cubo. These results suggested that the specific features of the Cuban fungal populations would be particularly useful for selecting germplasm sources for more durable blast-resistance breeding. Análisis de la Diversidad Genética de Poblaciones del Patógeno del Añublo del Arroz en Dos Localidades de Cuba. Fue estudiada la estructura genética de poblaciones del patógeno del añublo del arroz en dos regiones arroceras localizodos en el occidente (Los Palacios) y en el centro (Sur del Jíbaro) del país, utilizando 149 y 107 aislados monoconidiales, respectivamente. El ADN de estos aislados, los cuales se colectaron de 35 genotipos de arroz, se digirió con lo enzima de restricción EcoRl y posteriormente se evalué con lo sondo de ADN, MGR586; lo cual ha sido ampliamente utilizada en análisis de poblaciones del este hongo. Se identificaron grupos de dactiloscopia de ADN-MGR sobre la bose de similitudes en el polimorfismo en el largo del fragmento de restricción de ADN. Este análisis permitió la identificación de 1 34 haplotipos distribuidos en cuatro grupos genéticos altamente relacionados. La similitud media entre grupos estuvo en un rango entre el 75 y el 85%. El grupo A fue el predominante en ambas localidades y estuvo compuesto por el 84.7% de todos los aislados monoconidiales, los cuales fueron obtenidos de 33 genotipos de arroz. Los aislados de los grupos B, C y D, presentes solo en lo región de Los Palacios, estuvieron asociados con uno o con un pequeño número de genotipos estrechamente relacionados. Los estimados de diversidad genética desarrollados entre colecciones del patógeno y dentro de estas, mostraron uno mayor diversidad en la población del patógeno de lo región arrocera de occidente. Este análisis además sugirió que pudieron ocurrir eventos de migración de lo región occidental del país hacia lo central. Los resultados obtenidos sugieren que lo información sobre poblaciones de este patógeno en Cuba puede ser particularmente útil poro el desarrollo variedades mejoradas con resistencia más duradera al añublo.. 2003 2014-09-24T08:42:12Z 2014-09-24T08:42:12Z Journal Article https://hdl.handle.net/10568/43486 en Open Access |
| spellingShingle | oryza sativa rice genetic variation pyricularia dna fingerprinting arroz variación genética huellas genéticas adn Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Mora, L Duque E., Myriam Cristina Deus, J.E. Cornide, MT Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title | Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title_full | Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title_fullStr | Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title_full_unstemmed | Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title_short | Genetic diversity analysis of the rice blast pathogen population of two locations in Cuba |
| title_sort | genetic diversity analysis of the rice blast pathogen population of two locations in cuba |
| topic | oryza sativa rice genetic variation pyricularia dna fingerprinting arroz variación genética huellas genéticas adn |
| url | https://hdl.handle.net/10568/43486 |
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