Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.)
Four multiplex panels of fluorescent-labeled rice microsatellite markers were designed to survey allelic diversity at a total of 27 simple sequence repeat loci. Automated fragment detection, size calling and binning were used to identify the microsatellite alleles. The panels were tested on 72 acces...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2002
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43464 |
| _version_ | 1855523804214722560 |
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| author | Blair, Matthew W. Hedetale, V McCouch, Susan R. |
| author_browse | Blair, Matthew W. Hedetale, V McCouch, Susan R. |
| author_facet | Blair, Matthew W. Hedetale, V McCouch, Susan R. |
| author_sort | Blair, Matthew W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Four multiplex panels of fluorescent-labeled rice microsatellite markers were designed to survey allelic diversity at a total of 27 simple sequence repeat loci. Automated fragment detection, size calling and binning were used to identify the microsatellite alleles. The panels were tested on 72 accessions representing the diversity of ecotypes and isozyme groups in cultivated rice (Oryza sativa L.). Genetic diversity was evaluated by estimating the distribution and range of allele sizes, as well as the polymorphism information content (PIC), for each microsatellite locus. The multiplex panels were shown to be useful for fingerprinting and for clustering rice varieties, and genetic associations determined in this analysis agreed well with previous isozyme- and RFLP-based studies of these genotypes. Because of the wide cross-section of germplasm represented, we expect that the allele size-ranges reported here are close to the maximum for cultivated rice. In the future, panels of microsatellites from each rice chromosome will be made to facilitate the mapping of segregating populations and the identification of genes and QTLs underlying traits of interest. |
| format | Journal Article |
| id | CGSpace43464 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace434642024-08-27T10:35:49Z Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) Blair, Matthew W. Hedetale, V McCouch, Susan R. oryza sativa rice microsatellites genetic variation genetic maps arroz microsatélites variación genética mapas genéticos Four multiplex panels of fluorescent-labeled rice microsatellite markers were designed to survey allelic diversity at a total of 27 simple sequence repeat loci. Automated fragment detection, size calling and binning were used to identify the microsatellite alleles. The panels were tested on 72 accessions representing the diversity of ecotypes and isozyme groups in cultivated rice (Oryza sativa L.). Genetic diversity was evaluated by estimating the distribution and range of allele sizes, as well as the polymorphism information content (PIC), for each microsatellite locus. The multiplex panels were shown to be useful for fingerprinting and for clustering rice varieties, and genetic associations determined in this analysis agreed well with previous isozyme- and RFLP-based studies of these genotypes. Because of the wide cross-section of germplasm represented, we expect that the allele size-ranges reported here are close to the maximum for cultivated rice. In the future, panels of microsatellites from each rice chromosome will be made to facilitate the mapping of segregating populations and the identification of genes and QTLs underlying traits of interest. 2002-08 2014-09-24T08:42:10Z 2014-09-24T08:42:10Z Journal Article https://hdl.handle.net/10568/43464 en Limited Access Springer |
| spellingShingle | oryza sativa rice microsatellites genetic variation genetic maps arroz microsatélites variación genética mapas genéticos Blair, Matthew W. Hedetale, V McCouch, Susan R. Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title | Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title_full | Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title_fullStr | Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title_full_unstemmed | Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title_short | Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) |
| title_sort | fluorescent labeled microsatellite panels useful for detecting allelic diversity in cultivated rice oryza sativa l |
| topic | oryza sativa rice microsatellites genetic variation genetic maps arroz microsatélites variación genética mapas genéticos |
| url | https://hdl.handle.net/10568/43464 |
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