Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America
A new disease of rice, known as ‘entorchamiento’ (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts and purified preparations from diseased rice plants, contained virus-like part...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Springer
1999
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| Acceso en línea: | https://hdl.handle.net/10568/43405 |
| _version_ | 1855521299693043712 |
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| author | Morales, Francisco José Ward, Elaine Castaño, Mauricio Arroyave, José A. Lozano Potes, Iván Adams, Michael J. |
| author_browse | Adams, Michael J. Arroyave, José A. Castaño, Mauricio Lozano Potes, Iván Morales, Francisco José Ward, Elaine |
| author_facet | Morales, Francisco José Ward, Elaine Castaño, Mauricio Arroyave, José A. Lozano Potes, Iván Adams, Michael J. |
| author_sort | Morales, Francisco José |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A new disease of rice, known as ‘entorchamiento’ (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts and purified preparations from diseased rice plants, contained virus-like particles ca. 20?nm in diameter, with a bimodal length of 260 and 360?nm. Particle aggregates were also observed in the cytoplasm of infected rice leaf cells. Electrophoretic analyses of purified preparations and dsRNA extracts, revealed a single protein species of M 22,500, and four dsRNA bands ca. 6300, 4600, 2700 and 1800?bp in size. Cystosori, characteristic of plasmodiophorid fungal vectors of plant viruses, were consistently observed in the roots of diseased rice plants. PCR and sequence analyses of amplified fungal DNA products from infected rice roots, revealed that the putative fungus vector was Polymyxa graminis. A Western blot of tissue extracts obtained from ‘crinkled’ rice plants from Colombia, using antiserum against a West African isolate of rice stripe necrosis furovirus (RSNV), resulted in the detection of a protein band of approximately M 22,000. The RSNV antiserum recognized the Colombian virus isolate in serologically specific electron microscopy tests. These results confirm the presence of RSNV in the Americas. |
| format | Journal Article |
| id | CGSpace43405 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace434052024-08-29T11:41:29Z Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America Morales, Francisco José Ward, Elaine Castaño, Mauricio Arroyave, José A. Lozano Potes, Iván Adams, Michael J. oryza sativa polymyxa furoviruses plasmodiophoromycets plant diseases furovirus plasmodiophoromycetes enfermedades de las plantas A new disease of rice, known as ‘entorchamiento’ (crinkling), was first noticed in the Department of Meta, Colombia, in 1991. Symptoms include seedling death, foliar striping and severe plant malformation. Tissue extracts and purified preparations from diseased rice plants, contained virus-like particles ca. 20?nm in diameter, with a bimodal length of 260 and 360?nm. Particle aggregates were also observed in the cytoplasm of infected rice leaf cells. Electrophoretic analyses of purified preparations and dsRNA extracts, revealed a single protein species of M 22,500, and four dsRNA bands ca. 6300, 4600, 2700 and 1800?bp in size. Cystosori, characteristic of plasmodiophorid fungal vectors of plant viruses, were consistently observed in the roots of diseased rice plants. PCR and sequence analyses of amplified fungal DNA products from infected rice roots, revealed that the putative fungus vector was Polymyxa graminis. A Western blot of tissue extracts obtained from ‘crinkled’ rice plants from Colombia, using antiserum against a West African isolate of rice stripe necrosis furovirus (RSNV), resulted in the detection of a protein band of approximately M 22,000. The RSNV antiserum recognized the Colombian virus isolate in serologically specific electron microscopy tests. These results confirm the presence of RSNV in the Americas. 1999 2014-09-24T08:42:05Z 2014-09-24T08:42:05Z Journal Article https://hdl.handle.net/10568/43405 en Limited Access Springer Morales, Francisco José; Ward, Elaine; Castaño, Mauricio; Arroyave, José A.; Lozano Potes, Iván; Adams, Michael J. (2000). Emergence and Partial Characterization of Rice Stripe Necrosis Virus and its Fungus Vector in South America. European Journal of Plant Pathology 105: 643-650 |
| spellingShingle | oryza sativa polymyxa furoviruses plasmodiophoromycets plant diseases furovirus plasmodiophoromycetes enfermedades de las plantas Morales, Francisco José Ward, Elaine Castaño, Mauricio Arroyave, José A. Lozano Potes, Iván Adams, Michael J. Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title | Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title_full | Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title_fullStr | Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title_full_unstemmed | Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title_short | Emergence and partial characterization of rice stripe necrosis virus and its fungus vector in South America |
| title_sort | emergence and partial characterization of rice stripe necrosis virus and its fungus vector in south america |
| topic | oryza sativa polymyxa furoviruses plasmodiophoromycets plant diseases furovirus plasmodiophoromycetes enfermedades de las plantas |
| url | https://hdl.handle.net/10568/43405 |
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