Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)
A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to li...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2003
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| Acceso en línea: | https://hdl.handle.net/10568/43330 |
| _version_ | 1855543112405876736 |
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| author | Blair, Matthew W. Pedraza García, Fabio Buendía Castellanos, Hector F. Gaitán Solís, Eliana Beebe, Stephen E. Gepts, Paul L. Tohme, Joseph M. |
| author_browse | Beebe, Stephen E. Blair, Matthew W. Buendía Castellanos, Hector F. Gaitán Solís, Eliana Gepts, Paul L. Pedraza García, Fabio Tohme, Joseph M. |
| author_facet | Blair, Matthew W. Pedraza García, Fabio Buendía Castellanos, Hector F. Gaitán Solís, Eliana Beebe, Stephen E. Gepts, Paul L. Tohme, Joseph M. |
| author_sort | Blair, Matthew W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to link two existing RFLP-based genetic maps with a base map developed for the Mesoamerican × Andean population, DOR364 × G19833. Two types of microsatellites were mapped, based respectively on gene-coding and anonymous genomic-sequences. Gene-based microsatellites proved to be less polymorphic (46.3%) than anonymous genomic microsatellites (64.3%) between the parents of two inter-genepool crosses. The majority of the microsatellites produced single bands and detected single loci, however four of the gene-based and three of the genomic microsatellites produced consistent double or multiple banding patterns and detected more than one locus. Microsatellite loci were found on each of the 11 chromosomes of common bean, the number per chromosome ranging from 5 to 17 with an average of ten microsatellites each. Total map length for the base map was 1,720 cM and the average chromosome length was 156.4 cM, with an average distance between microsatellite loci of 19.5 cM. The development of new microsatellites from sequences in the Genbank database and the implication of these results for genetic mapping, quantitative trait locus analysis and marker-assisted selection in common bean are described. |
| format | Journal Article |
| id | CGSpace43330 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| record_format | dspace |
| spelling | CGSpace433302023-06-13T06:42:05Z Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) Blair, Matthew W. Pedraza García, Fabio Buendía Castellanos, Hector F. Gaitán Solís, Eliana Beebe, Stephen E. Gepts, Paul L. Tohme, Joseph M. phaseolus vulgaris genetic markers microsatellites genetic maps genetic polymorphism marcadores genéticos microsatélites mapas genéticos polimorfismo genético A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to link two existing RFLP-based genetic maps with a base map developed for the Mesoamerican × Andean population, DOR364 × G19833. Two types of microsatellites were mapped, based respectively on gene-coding and anonymous genomic-sequences. Gene-based microsatellites proved to be less polymorphic (46.3%) than anonymous genomic microsatellites (64.3%) between the parents of two inter-genepool crosses. The majority of the microsatellites produced single bands and detected single loci, however four of the gene-based and three of the genomic microsatellites produced consistent double or multiple banding patterns and detected more than one locus. Microsatellite loci were found on each of the 11 chromosomes of common bean, the number per chromosome ranging from 5 to 17 with an average of ten microsatellites each. Total map length for the base map was 1,720 cM and the average chromosome length was 156.4 cM, with an average distance between microsatellite loci of 19.5 cM. The development of new microsatellites from sequences in the Genbank database and the implication of these results for genetic mapping, quantitative trait locus analysis and marker-assisted selection in common bean are described. 2003 2014-09-24T08:41:58Z 2014-09-24T08:41:58Z Journal Article https://hdl.handle.net/10568/43330 en Open Access Blair, Matthew W.; Pedraza García, Fabio; Buendía Castellanos, Héctor Fabio; Gaitán Solís, Eliana; Beebe, Stephen E.; Gepts, Paul L.; Tohme M., Joseph. 2003. Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) . Theoretical and Applied Genetics (Germany) 107(8):1362-1374. |
| spellingShingle | phaseolus vulgaris genetic markers microsatellites genetic maps genetic polymorphism marcadores genéticos microsatélites mapas genéticos polimorfismo genético Blair, Matthew W. Pedraza García, Fabio Buendía Castellanos, Hector F. Gaitán Solís, Eliana Beebe, Stephen E. Gepts, Paul L. Tohme, Joseph M. Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title | Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title_full | Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title_fullStr | Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title_full_unstemmed | Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title_short | Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.) |
| title_sort | development of a genome wide anchored microsatellite map for common bean phaseolus vulgaris l |
| topic | phaseolus vulgaris genetic markers microsatellites genetic maps genetic polymorphism marcadores genéticos microsatélites mapas genéticos polimorfismo genético |
| url | https://hdl.handle.net/10568/43330 |
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