Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops
Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrif...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2007
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43329 |
| _version_ | 1855526455160602624 |
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| author | González Amao, M.T. Panta, A. Roca, W. Escobar Pérez, Roosevelt H. Engelmann, F. |
| author_browse | Engelmann, F. Escobar Pérez, Roosevelt H. González Amao, M.T. Panta, A. Roca, W. |
| author_facet | González Amao, M.T. Panta, A. Roca, W. Escobar Pérez, Roosevelt H. Engelmann, F. |
| author_sort | González Amao, M.T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks. |
| format | Journal Article |
| id | CGSpace43329 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace433292024-08-27T10:36:04Z Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops González Amao, M.T. Panta, A. Roca, W. Escobar Pérez, Roosevelt H. Engelmann, F. cryopreservation somatic embryogenesis criopreservación embriogénesis somática Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks. 2007-11-29 2014-09-24T08:41:58Z 2014-09-24T08:41:58Z Journal Article https://hdl.handle.net/10568/43329 en Limited Access Springer |
| spellingShingle | cryopreservation somatic embryogenesis criopreservación embriogénesis somática González Amao, M.T. Panta, A. Roca, W. Escobar Pérez, Roosevelt H. Engelmann, F. Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title | Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title_full | Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title_fullStr | Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title_full_unstemmed | Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title_short | Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| title_sort | development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops |
| topic | cryopreservation somatic embryogenesis criopreservación embriogénesis somática |
| url | https://hdl.handle.net/10568/43329 |
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