Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and sequ...

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Bibliographic Details
Main Authors: Suarez, MC, Bernal, A., Gutiérrez, J., Tohme, Joseph M., Fregene, Martin A.
Format: Journal Article
Language:Inglés
Published: Canadian Science Publishing 2000
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Online Access:https://hdl.handle.net/10568/43327

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