Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of B...

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Autores principales: Hidayat, SH, Gilbertson, R.L., Hanson, SF, Morales, Francisco José, Ahlquist, P.G., Russell, D.R., Maxwell, D.P.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Scientific Societies 1993
Materias:
Acceso en línea:https://hdl.handle.net/10568/43293
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author Hidayat, SH
Gilbertson, R.L.
Hanson, SF
Morales, Francisco José
Ahlquist, P.G.
Russell, D.R.
Maxwell, D.P.
author_browse Ahlquist, P.G.
Gilbertson, R.L.
Hanson, SF
Hidayat, SH
Maxwell, D.P.
Morales, Francisco José
Russell, D.R.
author_facet Hidayat, SH
Gilbertson, R.L.
Hanson, SF
Morales, Francisco José
Ahlquist, P.G.
Russell, D.R.
Maxwell, D.P.
author_sort Hidayat, SH
collection Repository of Agricultural Research Outputs (CGSpace)
description Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of BDMV resembles that of other whitefly-transmitted geminiviruses. The DNA-A (2,615 nt) and DNA-B (2,575 nt) have little sequence homology other than that within the common region. The nucleotide sequences of the 187-nt common regions of the two DNAs had 96% identity and included a region of 33 nt that can form a stem-loop structure nearly identical to that of other geminiviruses. Nucleotide sequence analysis identified six open reading frames, four on DNA-A and two on DNA-B, involving viral and complementary strand sense transcription. Comparisons of nucleotide and derived amino acid sequences of BDMV with those of other whitefly-transmitted geminiviruses showed that BDMV was most closely related to Abutilon mosaic geminivirus and not closely related to bean golden mosaic geminivirus. BDMV can be distinguished from other bean-infecting geminiviruses by differences in symptoms, host range, reaction of bean germplasm, and sequence identities; thus, BDMV is a distinct bean-infecting geminivirus.
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spelling CGSpace432932024-05-01T08:20:11Z Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus Hidayat, SH Gilbertson, R.L. Hanson, SF Morales, Francisco José Ahlquist, P.G. Russell, D.R. Maxwell, D.P. phaseolus vulgaris geminiviruses nucleotide sequence dna geminivirus secuencia nucleotídica adn Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of BDMV resembles that of other whitefly-transmitted geminiviruses. The DNA-A (2,615 nt) and DNA-B (2,575 nt) have little sequence homology other than that within the common region. The nucleotide sequences of the 187-nt common regions of the two DNAs had 96% identity and included a region of 33 nt that can form a stem-loop structure nearly identical to that of other geminiviruses. Nucleotide sequence analysis identified six open reading frames, four on DNA-A and two on DNA-B, involving viral and complementary strand sense transcription. Comparisons of nucleotide and derived amino acid sequences of BDMV with those of other whitefly-transmitted geminiviruses showed that BDMV was most closely related to Abutilon mosaic geminivirus and not closely related to bean golden mosaic geminivirus. BDMV can be distinguished from other bean-infecting geminiviruses by differences in symptoms, host range, reaction of bean germplasm, and sequence identities; thus, BDMV is a distinct bean-infecting geminivirus. 1993 2014-09-24T08:41:55Z 2014-09-24T08:41:55Z Journal Article https://hdl.handle.net/10568/43293 en Open Access Scientific Societies
spellingShingle phaseolus vulgaris
geminiviruses
nucleotide sequence
dna
geminivirus
secuencia nucleotídica
adn
Hidayat, SH
Gilbertson, R.L.
Hanson, SF
Morales, Francisco José
Ahlquist, P.G.
Russell, D.R.
Maxwell, D.P.
Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title_full Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title_fullStr Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title_full_unstemmed Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title_short Complete nucleotide sequences of the infectious cloned DNAs of bean dwarf mosaic geminivirus
title_sort complete nucleotide sequences of the infectious cloned dnas of bean dwarf mosaic geminivirus
topic phaseolus vulgaris
geminiviruses
nucleotide sequence
dna
geminivirus
secuencia nucleotídica
adn
url https://hdl.handle.net/10568/43293
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